Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1215 and GFF1216 are separated by 890 nucleotides GFF1216 and GFF1217 are separated by 463 nucleotides GFF1217 and GFF1218 are separated by 258 nucleotides
GFF1215 - Kappa-fimbriae regulatory protein, at 54,585 to 54,887
GFF1215
GFF1216 - protein YgiW precursor, at 55,778 to 55,987
GFF1216
GFF1217 - RepFIB associated resolvase, at 56,451 to 56,600
GFF1217
GFF1218 - RepFIB replication protein A, at 56,859 to 57,848
GFF1218
Position (kb) 55
56 Strain fitness (log2 ratio) -1
0
1
2
3 at 55.271 kb on + strand at 55.425 kb on + strand at 55.715 kb on - strand at 55.799 kb on - strand, within GFF1216 at 55.854 kb on - strand, within GFF1216 at 55.915 kb on + strand, within GFF1216 at 55.919 kb on - strand, within GFF1216 at 55.919 kb on - strand, within GFF1216 at 55.950 kb on + strand, within GFF1216 at 55.957 kb on - strand, within GFF1216 at 56.011 kb on + strand at 56.012 kb on - strand at 56.012 kb on - strand at 56.013 kb on + strand at 56.036 kb on - strand at 56.046 kb on + strand at 56.070 kb on - strand at 56.234 kb on - strand at 56.234 kb on - strand at 56.235 kb on + strand at 56.235 kb on + strand at 56.235 kb on + strand at 56.235 kb on + strand at 56.235 kb on + strand at 56.235 kb on + strand at 56.235 kb on + strand at 56.236 kb on - strand at 56.236 kb on - strand at 56.236 kb on - strand at 56.236 kb on - strand at 56.240 kb on + strand at 56.240 kb on + strand at 56.240 kb on + strand at 56.240 kb on + strand at 56.241 kb on - strand at 56.241 kb on - strand at 56.241 kb on - strand at 56.241 kb on - strand at 56.241 kb on - strand at 56.241 kb on - strand at 56.326 kb on - strand at 56.394 kb on + strand at 56.412 kb on + strand at 56.412 kb on + strand at 56.412 kb on + strand at 56.412 kb on + strand at 56.413 kb on - strand at 56.413 kb on - strand at 56.414 kb on + strand at 56.415 kb on - strand at 56.430 kb on + strand at 56.499 kb on + strand, within GFF1217 at 56.500 kb on - strand, within GFF1217 at 56.500 kb on - strand, within GFF1217 at 56.500 kb on - strand, within GFF1217 at 56.627 kb on - strand at 56.627 kb on - strand at 56.627 kb on - strand at 56.638 kb on - strand at 56.655 kb on - strand at 56.656 kb on + strand at 56.656 kb on + strand at 56.657 kb on - strand at 56.657 kb on - strand at 56.657 kb on - strand at 56.657 kb on - strand at 56.657 kb on - strand at 56.768 kb on - strand at 56.768 kb on - strand at 56.769 kb on + strand at 56.770 kb on - strand at 56.770 kb on - strand at 56.770 kb on - strand at 56.771 kb on + strand at 56.772 kb on - strand at 56.772 kb on - strand at 56.772 kb on - strand at 56.807 kb on - strand at 56.811 kb on + strand at 56.811 kb on + strand at 56.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 55,271 + -0.6 55,425 + -0.8 55,715 - -0.5 55,799 - GFF1216 0.10 +0.6 55,854 - GFF1216 0.36 -0.1 55,915 + GFF1216 0.65 -0.7 55,919 - GFF1216 0.67 +2.5 55,919 - GFF1216 0.67 +0.0 55,950 + GFF1216 0.82 -0.9 55,957 - GFF1216 0.85 +1.3 56,011 + -0.3 56,012 - -0.0 56,012 - +1.7 56,013 + -0.3 56,036 - +0.9 56,046 + +0.0 56,070 - +1.8 56,234 - +3.1 56,234 - +0.3 56,235 + +0.7 56,235 + -0.1 56,235 + +0.8 56,235 + -0.9 56,235 + -1.0 56,235 + -0.7 56,235 + +0.4 56,236 - +0.5 56,236 - -1.2 56,236 - -0.6 56,236 - +0.7 56,240 + +0.4 56,240 + +0.7 56,240 + -0.7 56,240 + -0.9 56,241 - +0.3 56,241 - -1.1 56,241 - -0.9 56,241 - -0.1 56,241 - -0.5 56,241 - -0.2 56,326 - -0.5 56,394 + -0.3 56,412 + -0.4 56,412 + +0.7 56,412 + -0.3 56,412 + +0.3 56,413 - +2.1 56,413 - +1.6 56,414 + -0.2 56,415 - -0.6 56,430 + -0.3 56,499 + GFF1217 0.32 +0.2 56,500 - GFF1217 0.33 -0.2 56,500 - GFF1217 0.33 -0.6 56,500 - GFF1217 0.33 -0.5 56,627 - +0.3 56,627 - +0.7 56,627 - -0.4 56,638 - -0.0 56,655 - +2.3 56,656 + -0.1 56,656 + -0.5 56,657 - +0.1 56,657 - -0.7 56,657 - +0.7 56,657 - +0.0 56,657 - -0.3 56,768 - -0.2 56,768 - -0.4 56,769 + -0.2 56,770 - +1.8 56,770 - +0.2 56,770 - +2.1 56,771 + +1.1 56,772 - +0.5 56,772 - -1.7 56,772 - +0.6 56,807 - -0.5 56,811 + -0.3 56,811 + -0.4 56,812 - +0.2
Or see this region's nucleotide sequence