Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1167 and GFF1168 are separated by 55 nucleotides GFF1168 and GFF1169 are separated by 41 nucleotides GFF1169 and GFF1170 overlap by 4 nucleotides GFF1170 and GFF1171 are separated by 87 nucleotides GFF1171 and GFF1172 overlap by 16 nucleotides GFF1172 and GFF1173 overlap by 33 nucleotides
GFF1167 - IncF plasmid conjugative transfer pilus assembly protein TraC, at 14,877 to 17,525
GFF1167
GFF1168 - IncF plasmid conjugative transfer protein TrbI, at 17,581 to 17,886
GFF1168
GFF1169 - IncF plasmid conjugative transfer pilus assembly protein TraW, at 17,928 to 18,515
GFF1169
GFF1170 - IncF plasmid conjugative transfer pilus assembly protein TraU, at 18,512 to 19,504
GFF1170
GFF1171 - Conjugative transfer protein PSLT093, at 19,592 to 19,831
GFF1171
GFF1172 - IncF plasmid conjugative transfer protein TrbC, at 19,816 to 20,454
GFF1172
GFF1173 - hypothetical protein, at 20,422 to 20,586
GFF1173
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 17.588 kb on + strand at 17.609 kb on - strand at 17.739 kb on + strand, within GFF1168 at 17.868 kb on + strand at 17.869 kb on - strand at 18.231 kb on + strand, within GFF1169 at 18.231 kb on + strand, within GFF1169 at 18.231 kb on + strand, within GFF1169 at 18.232 kb on - strand, within GFF1169 at 18.255 kb on + strand, within GFF1169 at 18.333 kb on - strand, within GFF1169 at 18.537 kb on + strand at 18.658 kb on + strand, within GFF1170 at 18.659 kb on - strand, within GFF1170 at 18.659 kb on - strand, within GFF1170 at 18.905 kb on + strand, within GFF1170 at 18.905 kb on + strand, within GFF1170 at 18.938 kb on + strand, within GFF1170 at 18.949 kb on + strand, within GFF1170 at 18.950 kb on - strand, within GFF1170 at 19.130 kb on - strand, within GFF1170 at 19.130 kb on - strand, within GFF1170 at 19.178 kb on + strand, within GFF1170 at 19.178 kb on + strand, within GFF1170 at 19.179 kb on - strand, within GFF1170 at 19.187 kb on - strand, within GFF1170 at 19.279 kb on + strand, within GFF1170 at 19.301 kb on - strand, within GFF1170 at 19.301 kb on - strand, within GFF1170 at 19.537 kb on + strand at 19.544 kb on - strand at 19.556 kb on + strand at 19.557 kb on - strand at 19.614 kb on + strand at 19.697 kb on - strand, within GFF1171 at 19.765 kb on + strand, within GFF1171 at 19.766 kb on - strand, within GFF1171 at 19.798 kb on + strand, within GFF1171 at 19.798 kb on + strand, within GFF1171 at 19.891 kb on - strand, within GFF1172 at 19.893 kb on + strand, within GFF1172 at 19.894 kb on - strand, within GFF1172 at 19.894 kb on - strand, within GFF1172 at 20.162 kb on + strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.321 kb on - strand, within GFF1172 at 20.321 kb on - strand, within GFF1172 at 20.335 kb on + strand, within GFF1172 at 20.335 kb on + strand, within GFF1172 at 20.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 17,588 + +3.2 17,609 - +1.5 17,739 + GFF1168 0.52 -0.7 17,868 + -0.3 17,869 - +0.3 18,231 + GFF1169 0.52 -0.8 18,231 + GFF1169 0.52 -1.3 18,231 + GFF1169 0.52 -0.8 18,232 - GFF1169 0.52 -0.2 18,255 + GFF1169 0.56 +0.1 18,333 - GFF1169 0.69 +0.3 18,537 + -1.0 18,658 + GFF1170 0.15 +0.8 18,659 - GFF1170 0.15 +2.3 18,659 - GFF1170 0.15 -0.6 18,905 + GFF1170 0.40 -0.6 18,905 + GFF1170 0.40 -0.4 18,938 + GFF1170 0.43 -0.1 18,949 + GFF1170 0.44 +2.4 18,950 - GFF1170 0.44 -1.4 19,130 - GFF1170 0.62 +2.6 19,130 - GFF1170 0.62 -0.2 19,178 + GFF1170 0.67 +0.6 19,178 + GFF1170 0.67 -1.3 19,179 - GFF1170 0.67 -0.6 19,187 - GFF1170 0.68 -0.1 19,279 + GFF1170 0.77 +2.6 19,301 - GFF1170 0.79 -1.0 19,301 - GFF1170 0.79 +1.8 19,537 + -1.0 19,544 - -1.1 19,556 + +0.6 19,557 - -0.1 19,614 + -0.2 19,697 - GFF1171 0.44 +1.6 19,765 + GFF1171 0.72 -0.2 19,766 - GFF1171 0.72 -0.6 19,798 + GFF1171 0.86 +0.6 19,798 + GFF1171 0.86 +2.3 19,891 - GFF1172 0.12 +0.8 19,893 + GFF1172 0.12 +1.4 19,894 - GFF1172 0.12 -0.2 19,894 - GFF1172 0.12 +0.3 20,162 + GFF1172 0.54 -0.5 20,288 - GFF1172 0.74 -1.9 20,288 - GFF1172 0.74 -0.1 20,288 - GFF1172 0.74 -0.7 20,321 - GFF1172 0.79 -0.5 20,321 - GFF1172 0.79 -2.1 20,335 + GFF1172 0.81 +1.3 20,335 + GFF1172 0.81 -0.7 20,443 - -0.8
Or see this region's nucleotide sequence