Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1039 and GFF1040 overlap by 4 nucleotides GFF1040 and GFF1041 are separated by 32 nucleotides GFF1041 and GFF1042 are separated by 100 nucleotides
GFF1039 - FIG00638703: hypothetical protein, at 220,262 to 221,335
GFF1039
GFF1040 - Putative deoxyribonuclease YjjV, at 221,332 to 222,105
GFF1040
GFF1041 - radical activating enzyme, at 222,138 to 222,872
GFF1041
GFF1042 - FIG00638118: hypothetical protein, at 222,973 to 224,523
GFF1042
Position (kb) 222
223 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 221.148 kb on - strand, within GFF1039 at 221.203 kb on + strand, within GFF1039 at 221.203 kb on + strand, within GFF1039 at 221.203 kb on + strand, within GFF1039 at 221.346 kb on + strand at 221.352 kb on + strand at 221.382 kb on + strand at 221.959 kb on - strand, within GFF1040 at 222.073 kb on + strand at 222.074 kb on - strand at 222.088 kb on - strand at 222.140 kb on - strand at 222.145 kb on + strand at 222.145 kb on + strand at 222.422 kb on + strand, within GFF1041 at 222.423 kb on - strand, within GFF1041 at 222.489 kb on + strand, within GFF1041 at 222.489 kb on + strand, within GFF1041 at 222.490 kb on - strand, within GFF1041 at 222.510 kb on + strand, within GFF1041 at 222.545 kb on + strand, within GFF1041 at 222.657 kb on - strand, within GFF1041 at 222.686 kb on + strand, within GFF1041 at 222.792 kb on + strand, within GFF1041 at 222.793 kb on - strand, within GFF1041 at 222.919 kb on - strand at 222.975 kb on - strand at 222.981 kb on + strand at 222.982 kb on - strand at 222.982 kb on - strand at 223.071 kb on + strand at 223.125 kb on + strand at 223.126 kb on - strand at 223.126 kb on - strand at 223.126 kb on - strand at 223.126 kb on - strand at 223.565 kb on + strand, within GFF1042 at 223.566 kb on - strand, within GFF1042 at 223.566 kb on - strand, within GFF1042 at 223.566 kb on - strand, within GFF1042 at 223.627 kb on - strand, within GFF1042 at 223.700 kb on + strand, within GFF1042 at 223.714 kb on - strand, within GFF1042 at 223.741 kb on - strand, within GFF1042 at 223.774 kb on + strand, within GFF1042 at 223.815 kb on - strand, within GFF1042
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 221,148 - GFF1039 0.82 +0.3 221,203 + GFF1039 0.88 -0.6 221,203 + GFF1039 0.88 -0.4 221,203 + GFF1039 0.88 +0.1 221,346 + +0.0 221,352 + +0.8 221,382 + -0.1 221,959 - GFF1040 0.81 +0.1 222,073 + +0.3 222,074 - +1.4 222,088 - -1.8 222,140 - +0.2 222,145 + +0.9 222,145 + +2.9 222,422 + GFF1041 0.39 -1.5 222,423 - GFF1041 0.39 +0.6 222,489 + GFF1041 0.48 +0.8 222,489 + GFF1041 0.48 +0.0 222,490 - GFF1041 0.48 +1.5 222,510 + GFF1041 0.51 -0.0 222,545 + GFF1041 0.55 -1.8 222,657 - GFF1041 0.71 -0.1 222,686 + GFF1041 0.75 -0.0 222,792 + GFF1041 0.89 -1.0 222,793 - GFF1041 0.89 -0.8 222,919 - -0.5 222,975 - +0.3 222,981 + +2.2 222,982 - +2.4 222,982 - -0.1 223,071 + +0.2 223,125 + +0.3 223,126 - +0.6 223,126 - -0.6 223,126 - +1.7 223,126 - -0.8 223,565 + GFF1042 0.38 -1.4 223,566 - GFF1042 0.38 -0.5 223,566 - GFF1042 0.38 +0.4 223,566 - GFF1042 0.38 -0.2 223,627 - GFF1042 0.42 -0.5 223,700 + GFF1042 0.47 +0.4 223,714 - GFF1042 0.48 -0.4 223,741 - GFF1042 0.50 +2.0 223,774 + GFF1042 0.52 +0.8 223,815 - GFF1042 0.54 +1.4
Or see this region's nucleotide sequence