Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1009 and GFF1010 are separated by 112 nucleotides GFF1010 and GFF1011 are separated by 61 nucleotides GFF1011 and GFF1012 are separated by 101 nucleotides GFF1012 and GFF1013 are separated by 14 nucleotides
GFF1009 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 193,434 to 195,095
GFF1009
GFF1010 - FIG01048313: hypothetical protein, at 195,208 to 195,357
GFF1010
GFF1011 - Transcriptional regulatory protein levR, at 195,419 to 198,184
GFF1011
GFF1012 - PTS system, mannose-specific IIA component (EC 2.7.1.69), at 198,286 to 198,708
GFF1012
GFF1013 - PTS system, mannose-specific IIB component (EC 2.7.1.69), at 198,723 to 199,184
GFF1013
Position (kb) 195
196
197
198
199 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 194.584 kb on + strand, within GFF1009 at 194.722 kb on + strand, within GFF1009 at 194.723 kb on - strand, within GFF1009 at 194.723 kb on - strand, within GFF1009 at 195.121 kb on - strand at 195.210 kb on - strand at 195.246 kb on - strand, within GFF1010 at 195.386 kb on + strand at 195.386 kb on + strand at 195.386 kb on + strand at 195.387 kb on - strand at 195.523 kb on + strand at 195.523 kb on + strand at 195.524 kb on - strand at 195.917 kb on - strand, within GFF1011 at 195.917 kb on - strand, within GFF1011 at 196.053 kb on + strand, within GFF1011 at 196.053 kb on + strand, within GFF1011 at 196.054 kb on - strand, within GFF1011 at 196.180 kb on - strand, within GFF1011 at 196.200 kb on + strand, within GFF1011 at 196.201 kb on - strand, within GFF1011 at 196.208 kb on - strand, within GFF1011 at 196.382 kb on + strand, within GFF1011 at 196.383 kb on - strand, within GFF1011 at 196.428 kb on - strand, within GFF1011 at 196.582 kb on + strand, within GFF1011 at 196.630 kb on + strand, within GFF1011 at 196.656 kb on - strand, within GFF1011 at 196.656 kb on - strand, within GFF1011 at 196.666 kb on - strand, within GFF1011 at 196.695 kb on + strand, within GFF1011 at 196.858 kb on + strand, within GFF1011 at 196.863 kb on - strand, within GFF1011 at 196.881 kb on + strand, within GFF1011 at 196.882 kb on - strand, within GFF1011 at 197.041 kb on + strand, within GFF1011 at 197.042 kb on - strand, within GFF1011 at 197.126 kb on + strand, within GFF1011 at 197.144 kb on - strand, within GFF1011 at 197.246 kb on + strand, within GFF1011 at 197.474 kb on + strand, within GFF1011 at 197.505 kb on + strand, within GFF1011 at 197.647 kb on - strand, within GFF1011 at 197.650 kb on + strand, within GFF1011 at 197.754 kb on - strand, within GFF1011 at 197.754 kb on - strand, within GFF1011 at 197.963 kb on + strand at 197.964 kb on - strand at 197.964 kb on - strand at 197.964 kb on - strand at 197.971 kb on + strand at 197.971 kb on + strand at 197.971 kb on + strand at 197.975 kb on - strand at 197.975 kb on - strand at 198.092 kb on + strand at 198.093 kb on - strand at 198.093 kb on - strand at 198.137 kb on - strand at 198.359 kb on + strand, within GFF1012 at 198.438 kb on - strand, within GFF1012 at 198.615 kb on + strand, within GFF1012 at 198.806 kb on + strand, within GFF1013 at 198.833 kb on + strand, within GFF1013
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 194,584 + GFF1009 0.69 +0.6 194,722 + GFF1009 0.77 +0.5 194,723 - GFF1009 0.78 -0.6 194,723 - GFF1009 0.78 -1.9 195,121 - -1.3 195,210 - -0.5 195,246 - GFF1010 0.25 +0.9 195,386 + +0.2 195,386 + -0.8 195,386 + -0.9 195,387 - +1.0 195,523 + +0.5 195,523 + -0.5 195,524 - -0.1 195,917 - GFF1011 0.18 -0.1 195,917 - GFF1011 0.18 -0.9 196,053 + GFF1011 0.23 +0.0 196,053 + GFF1011 0.23 +1.6 196,054 - GFF1011 0.23 +0.4 196,180 - GFF1011 0.28 +0.6 196,200 + GFF1011 0.28 -0.0 196,201 - GFF1011 0.28 -0.7 196,208 - GFF1011 0.29 +0.1 196,382 + GFF1011 0.35 +0.6 196,383 - GFF1011 0.35 -0.7 196,428 - GFF1011 0.36 -0.1 196,582 + GFF1011 0.42 -0.5 196,630 + GFF1011 0.44 -0.2 196,656 - GFF1011 0.45 +0.4 196,656 - GFF1011 0.45 +0.3 196,666 - GFF1011 0.45 -0.1 196,695 + GFF1011 0.46 +0.9 196,858 + GFF1011 0.52 +0.5 196,863 - GFF1011 0.52 -0.3 196,881 + GFF1011 0.53 -0.5 196,882 - GFF1011 0.53 +0.1 197,041 + GFF1011 0.59 -0.6 197,042 - GFF1011 0.59 -1.0 197,126 + GFF1011 0.62 +3.3 197,144 - GFF1011 0.62 +0.5 197,246 + GFF1011 0.66 -0.7 197,474 + GFF1011 0.74 -0.8 197,505 + GFF1011 0.75 +1.2 197,647 - GFF1011 0.81 +0.9 197,650 + GFF1011 0.81 -0.7 197,754 - GFF1011 0.84 +0.1 197,754 - GFF1011 0.84 -1.3 197,963 + -0.9 197,964 - +0.4 197,964 - -1.5 197,964 - +0.2 197,971 + +1.2 197,971 + +1.0 197,971 + +1.9 197,975 - +1.5 197,975 - +0.3 198,092 + -0.7 198,093 - -0.1 198,093 - -0.2 198,137 - -0.6 198,359 + GFF1012 0.17 -0.1 198,438 - GFF1012 0.36 +0.4 198,615 + GFF1012 0.78 -0.2 198,806 + GFF1013 0.18 +0.8 198,833 + GFF1013 0.24 +0.4
Or see this region's nucleotide sequence