Experiment: undecene 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeT and hlpA are separated by 121 nucleotides hlpA and lpxD are separated by 3 nucleotides lpxD and fabZ are separated by 104 nucleotides fabZ and lpxA are separated by 3 nucleotides
b0177: yaeT - hypothetical protein (NCBI), at 197,928 to 200,360
yaeT
b0178: hlpA - periplasmic chaperone (NCBI), at 200,482 to 200,967
hlpA
b0179: lpxD - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (NCBI), at 200,971 to 201,996
lpxD
b0180: fabZ - (3R)-hydroxymyristoyl ACP dehydratase (NCBI), at 202,101 to 202,556
fabZ
b0181: lpxA - UDP-N-acetylglucosamine acyltransferase (NCBI), at 202,560 to 203,348
lpxA
Position (kb)
200
201
202 Strain fitness (log2 ratio)
-2
-1
0
1 at 200.394 kb on + strand at 200.394 kb on + strand at 200.394 kb on + strand at 200.414 kb on + strand at 200.414 kb on + strand at 200.439 kb on + strand at 200.523 kb on + strand at 200.523 kb on + strand at 200.635 kb on + strand, within hlpA at 200.635 kb on + strand, within hlpA at 200.649 kb on + strand, within hlpA at 200.785 kb on + strand, within hlpA at 200.834 kb on + strand, within hlpA at 200.903 kb on + strand, within hlpA at 200.903 kb on + strand, within hlpA at 200.924 kb on + strand at 201.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction undecene 2.5 vol% remove 200,394 + +0.5 200,394 + +1.7 200,394 + +0.9 200,414 + +1.1 200,414 + +0.7 200,439 + +1.4 200,523 + -1.9 200,523 + -2.2 200,635 + hlpA b0178 0.31 +1.3 200,635 + hlpA b0178 0.31 -0.0 200,649 + hlpA b0178 0.34 -1.6 200,785 + hlpA b0178 0.62 -1.8 200,834 + hlpA b0178 0.72 -0.9 200,903 + hlpA b0178 0.87 -0.3 200,903 + hlpA b0178 0.87 -1.6 200,924 + -1.2 201,990 + +1.5
Or see this region's nucleotide sequence