Strain Fitness in Escherichia coli BW25113 around b0179

Experiment: undecene 2.5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyaeT and hlpA are separated by 121 nucleotideshlpA and lpxD are separated by 3 nucleotideslpxD and fabZ are separated by 104 nucleotidesfabZ and lpxA are separated by 3 nucleotides b0177: yaeT - hypothetical protein (NCBI), at 197,928 to 200,360 yaeT b0178: hlpA - periplasmic chaperone (NCBI), at 200,482 to 200,967 hlpA b0179: lpxD - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (NCBI), at 200,971 to 201,996 lpxD b0180: fabZ - (3R)-hydroxymyristoyl ACP dehydratase (NCBI), at 202,101 to 202,556 fabZ b0181: lpxA - UDP-N-acetylglucosamine acyltransferase (NCBI), at 202,560 to 203,348 lpxA Position (kb) 200 201 202Strain fitness (log2 ratio) -2 -1 0 1at 200.394 kb on + strandat 200.394 kb on + strandat 200.394 kb on + strandat 200.414 kb on + strandat 200.414 kb on + strandat 200.439 kb on + strandat 200.523 kb on + strandat 200.523 kb on + strandat 200.635 kb on + strand, within hlpAat 200.635 kb on + strand, within hlpAat 200.649 kb on + strand, within hlpAat 200.785 kb on + strand, within hlpAat 200.834 kb on + strand, within hlpAat 200.903 kb on + strand, within hlpAat 200.903 kb on + strand, within hlpAat 200.924 kb on + strandat 201.990 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction undecene 2.5 vol%
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200,394 + +0.5
200,394 + +1.7
200,394 + +0.9
200,414 + +1.1
200,414 + +0.7
200,439 + +1.4
200,523 + -1.9
200,523 + -2.2
200,635 + hlpA b0178 0.31 +1.3
200,635 + hlpA b0178 0.31 -0.0
200,649 + hlpA b0178 0.34 -1.6
200,785 + hlpA b0178 0.62 -1.8
200,834 + hlpA b0178 0.72 -0.9
200,903 + hlpA b0178 0.87 -0.3
200,903 + hlpA b0178 0.87 -1.6
200,924 + -1.2
201,990 + +1.5

Or see this region's nucleotide sequence