Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF887 and GFF888 are separated by 24 nucleotides GFF888 and GFF889 are separated by 497 nucleotides
GFF887 - 5-deoxy-glucuronate isomerase (EC 5.3.1.-), at 66,455 to 67,264
GFF887
GFF888 - Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27), at 67,289 to 68,794
GFF888
GFF889 - putative DNA-binding protein, at 69,292 to 70,059
GFF889
Position (kb) 67
68
69 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 66.322 kb on - strand at 66.330 kb on + strand at 66.330 kb on + strand at 66.330 kb on + strand at 66.331 kb on - strand at 66.331 kb on - strand at 66.331 kb on - strand at 66.332 kb on + strand at 66.332 kb on + strand at 66.333 kb on - strand at 66.335 kb on - strand at 66.456 kb on + strand at 66.479 kb on - strand at 66.547 kb on - strand, within GFF887 at 66.549 kb on + strand, within GFF887 at 66.550 kb on - strand, within GFF887 at 66.550 kb on - strand, within GFF887 at 66.553 kb on + strand, within GFF887 at 66.553 kb on + strand, within GFF887 at 66.554 kb on - strand, within GFF887 at 66.593 kb on - strand, within GFF887 at 66.606 kb on - strand, within GFF887 at 66.629 kb on - strand, within GFF887 at 66.705 kb on + strand, within GFF887 at 66.706 kb on - strand, within GFF887 at 66.788 kb on - strand, within GFF887 at 66.793 kb on - strand, within GFF887 at 66.793 kb on - strand, within GFF887 at 66.914 kb on + strand, within GFF887 at 67.185 kb on + strand at 67.287 kb on + strand at 67.288 kb on - strand at 67.290 kb on + strand at 67.372 kb on - strand at 67.521 kb on + strand, within GFF888 at 67.583 kb on - strand, within GFF888 at 67.597 kb on - strand, within GFF888 at 67.695 kb on + strand, within GFF888 at 67.869 kb on - strand, within GFF888 at 68.063 kb on + strand, within GFF888 at 68.093 kb on - strand, within GFF888 at 68.095 kb on - strand, within GFF888 at 68.095 kb on - strand, within GFF888 at 68.098 kb on + strand, within GFF888 at 68.098 kb on + strand, within GFF888 at 68.098 kb on + strand, within GFF888 at 68.098 kb on + strand, within GFF888 at 68.099 kb on - strand, within GFF888 at 68.099 kb on - strand, within GFF888 at 68.099 kb on - strand, within GFF888 at 68.099 kb on - strand, within GFF888 at 68.141 kb on + strand, within GFF888 at 68.243 kb on - strand, within GFF888 at 68.326 kb on + strand, within GFF888 at 68.327 kb on - strand, within GFF888 at 68.327 kb on - strand, within GFF888 at 69.291 kb on + strand at 69.292 kb on - strand at 69.344 kb on - strand at 69.395 kb on - strand, within GFF889 at 69.451 kb on - strand, within GFF889 at 69.605 kb on + strand, within GFF889 at 69.641 kb on + strand, within GFF889 at 69.652 kb on + strand, within GFF889
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 66,322 - +0.3 66,330 + -0.5 66,330 + -0.1 66,330 + +0.2 66,331 - +1.2 66,331 - +0.3 66,331 - -0.6 66,332 + -0.5 66,332 + -0.3 66,333 - -0.1 66,335 - +0.6 66,456 + -0.4 66,479 - -0.4 66,547 - GFF887 0.11 +0.6 66,549 + GFF887 0.12 -2.4 66,550 - GFF887 0.12 -0.1 66,550 - GFF887 0.12 +0.7 66,553 + GFF887 0.12 +0.4 66,553 + GFF887 0.12 -0.0 66,554 - GFF887 0.12 -0.2 66,593 - GFF887 0.17 +0.2 66,606 - GFF887 0.19 -0.8 66,629 - GFF887 0.21 -0.1 66,705 + GFF887 0.31 +0.9 66,706 - GFF887 0.31 +0.1 66,788 - GFF887 0.41 -0.4 66,793 - GFF887 0.42 +3.8 66,793 - GFF887 0.42 -0.5 66,914 + GFF887 0.57 +0.3 67,185 + +0.4 67,287 + -0.5 67,288 - +1.0 67,290 + -0.9 67,372 - -0.4 67,521 + GFF888 0.15 -0.9 67,583 - GFF888 0.20 -0.0 67,597 - GFF888 0.20 +0.1 67,695 + GFF888 0.27 +0.1 67,869 - GFF888 0.39 -0.1 68,063 + GFF888 0.51 -0.4 68,093 - GFF888 0.53 -0.1 68,095 - GFF888 0.54 -0.5 68,095 - GFF888 0.54 -0.5 68,098 + GFF888 0.54 -1.1 68,098 + GFF888 0.54 +0.2 68,098 + GFF888 0.54 -0.1 68,098 + GFF888 0.54 +0.5 68,099 - GFF888 0.54 -0.0 68,099 - GFF888 0.54 +0.6 68,099 - GFF888 0.54 +0.2 68,099 - GFF888 0.54 -0.0 68,141 + GFF888 0.57 -1.0 68,243 - GFF888 0.63 -0.2 68,326 + GFF888 0.69 -0.3 68,327 - GFF888 0.69 -0.6 68,327 - GFF888 0.69 +0.3 69,291 + +0.1 69,292 - -0.9 69,344 - +0.3 69,395 - GFF889 0.13 +0.2 69,451 - GFF889 0.21 -0.1 69,605 + GFF889 0.41 -0.6 69,641 + GFF889 0.45 +0.3 69,652 + GFF889 0.47 -0.6
Or see this region's nucleotide sequence