Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF645 and GFF646 are separated by 475 nucleotides GFF646 and GFF647 overlap by 1 nucleotides GFF647 and GFF648 are separated by 90 nucleotides
GFF645 - Membrane protein associated with oxaloacetate decarboxylase, at 403 to 1,101
GFF645
GFF646 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 1,577 to 2,560
GFF646
GFF647 - Ferrichrome transport ATP-binding protein FhuC (TC 3.A.1.14.3), at 2,560 to 3,336
GFF647
GFF648 - Phosphoglycerate mutase (EC 5.4.2.1), at 3,427 to 4,179
GFF648
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-1
0
1
2
3 at 0.725 kb on + strand, within GFF645 at 1.123 kb on + strand at 1.123 kb on + strand at 1.199 kb on - strand at 1.366 kb on - strand at 1.366 kb on - strand at 1.447 kb on + strand at 1.506 kb on + strand at 1.581 kb on + strand at 1.701 kb on + strand, within GFF646 at 1.888 kb on + strand, within GFF646 at 1.969 kb on + strand, within GFF646 at 1.969 kb on + strand, within GFF646 at 2.057 kb on - strand, within GFF646 at 2.164 kb on + strand, within GFF646 at 2.288 kb on - strand, within GFF646 at 2.338 kb on + strand, within GFF646 at 2.339 kb on - strand, within GFF646 at 2.370 kb on + strand, within GFF646 at 2.464 kb on + strand at 2.535 kb on + strand at 2.535 kb on + strand at 2.536 kb on - strand at 2.686 kb on - strand, within GFF647 at 2.696 kb on + strand, within GFF647 at 2.829 kb on - strand, within GFF647 at 2.840 kb on + strand, within GFF647 at 2.861 kb on + strand, within GFF647 at 2.909 kb on + strand, within GFF647 at 2.936 kb on + strand, within GFF647 at 2.957 kb on - strand, within GFF647 at 2.991 kb on - strand, within GFF647 at 3.083 kb on + strand, within GFF647 at 3.083 kb on + strand, within GFF647 at 3.120 kb on + strand, within GFF647 at 3.150 kb on + strand, within GFF647 at 3.154 kb on - strand, within GFF647 at 3.189 kb on - strand, within GFF647 at 3.342 kb on - strand at 3.342 kb on - strand at 3.404 kb on - strand at 3.404 kb on - strand at 3.404 kb on - strand at 3.497 kb on + strand at 3.498 kb on - strand at 3.498 kb on - strand at 3.533 kb on + strand, within GFF648 at 3.533 kb on + strand, within GFF648
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 725 + GFF645 0.46 -0.7 1,123 + +0.1 1,123 + -0.2 1,199 - +1.0 1,366 - -0.3 1,366 - +0.1 1,447 + -0.3 1,506 + +1.9 1,581 + -0.2 1,701 + GFF646 0.13 +1.1 1,888 + GFF646 0.32 +0.1 1,969 + GFF646 0.40 +2.9 1,969 + GFF646 0.40 -0.0 2,057 - GFF646 0.49 -0.2 2,164 + GFF646 0.60 +0.3 2,288 - GFF646 0.72 -0.1 2,338 + GFF646 0.77 +1.1 2,339 - GFF646 0.77 +1.9 2,370 + GFF646 0.81 -0.3 2,464 + +1.4 2,535 + +0.9 2,535 + +0.3 2,536 - -0.2 2,686 - GFF647 0.16 +0.4 2,696 + GFF647 0.18 +0.3 2,829 - GFF647 0.35 -0.4 2,840 + GFF647 0.36 +0.1 2,861 + GFF647 0.39 +0.4 2,909 + GFF647 0.45 -0.4 2,936 + GFF647 0.48 +0.4 2,957 - GFF647 0.51 +1.1 2,991 - GFF647 0.55 +0.2 3,083 + GFF647 0.67 -0.1 3,083 + GFF647 0.67 -0.5 3,120 + GFF647 0.72 -0.1 3,150 + GFF647 0.76 +0.8 3,154 - GFF647 0.76 -0.5 3,189 - GFF647 0.81 -0.4 3,342 - +0.6 3,342 - -0.3 3,404 - +0.7 3,404 - +0.9 3,404 - -0.1 3,497 + +0.2 3,498 - +0.2 3,498 - -0.5 3,533 + GFF648 0.14 -0.3 3,533 + GFF648 0.14 -1.3
Or see this region's nucleotide sequence