Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF623 and GFF624 are separated by 120 nucleotides GFF624 and GFF625 overlap by 1 nucleotides GFF625 and GFF626 overlap by 17 nucleotides
GFF623 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 363,877 to 366,429
GFF623
GFF624 - Type IV pilus biogenesis protein PilM, at 366,550 to 367,329
GFF624
GFF625 - Type IV pilus biogenesis protein PilN, at 367,329 to 367,868
GFF625
GFF626 - Type IV pilus biogenesis protein PilO, at 367,852 to 368,325
GFF626
Position (kb) 366
367
368 Strain fitness (log2 ratio) -1
0
1
2 at 365.612 kb on + strand, within GFF623 at 365.612 kb on + strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.642 kb on + strand, within GFF623 at 365.688 kb on + strand, within GFF623 at 365.689 kb on - strand, within GFF623 at 365.728 kb on - strand, within GFF623 at 365.728 kb on - strand, within GFF623 at 365.729 kb on + strand, within GFF623 at 365.741 kb on - strand, within GFF623 at 365.759 kb on - strand, within GFF623 at 365.803 kb on - strand, within GFF623 at 365.808 kb on + strand, within GFF623 at 365.856 kb on + strand, within GFF623 at 365.857 kb on - strand, within GFF623 at 365.857 kb on - strand, within GFF623 at 365.928 kb on - strand, within GFF623 at 365.934 kb on - strand, within GFF623 at 366.073 kb on - strand, within GFF623 at 366.073 kb on - strand, within GFF623 at 366.129 kb on + strand, within GFF623 at 366.159 kb on - strand, within GFF623 at 366.248 kb on + strand at 366.259 kb on - strand at 366.279 kb on - strand at 366.331 kb on + strand at 366.346 kb on - strand at 366.363 kb on - strand at 366.410 kb on - strand at 366.414 kb on - strand at 366.484 kb on + strand at 366.484 kb on + strand at 366.486 kb on + strand at 366.572 kb on + strand at 366.583 kb on - strand at 366.583 kb on - strand at 366.723 kb on - strand, within GFF624 at 366.723 kb on - strand, within GFF624 at 366.752 kb on + strand, within GFF624 at 366.766 kb on + strand, within GFF624 at 366.927 kb on - strand, within GFF624 at 367.041 kb on - strand, within GFF624 at 367.059 kb on + strand, within GFF624 at 367.062 kb on - strand, within GFF624 at 367.204 kb on - strand, within GFF624 at 367.322 kb on + strand at 367.322 kb on + strand at 367.323 kb on - strand at 367.371 kb on + strand at 367.372 kb on - strand at 367.376 kb on - strand at 367.534 kb on + strand, within GFF625 at 367.535 kb on - strand, within GFF625 at 367.619 kb on + strand, within GFF625 at 367.619 kb on + strand, within GFF625 at 367.620 kb on - strand, within GFF625 at 367.620 kb on - strand, within GFF625 at 367.677 kb on + strand, within GFF625 at 367.870 kb on - strand at 368.265 kb on + strand, within GFF626
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 365,612 + GFF623 0.68 +0.2 365,612 + GFF623 0.68 -0.3 365,613 - GFF623 0.68 -0.1 365,613 - GFF623 0.68 -0.3 365,613 - GFF623 0.68 +0.0 365,613 - GFF623 0.68 +0.1 365,642 + GFF623 0.69 -0.3 365,688 + GFF623 0.71 -0.5 365,689 - GFF623 0.71 -0.6 365,728 - GFF623 0.73 -0.0 365,728 - GFF623 0.73 -0.3 365,729 + GFF623 0.73 +0.2 365,741 - GFF623 0.73 +0.0 365,759 - GFF623 0.74 -0.2 365,803 - GFF623 0.75 +0.0 365,808 + GFF623 0.76 +0.2 365,856 + GFF623 0.78 +0.8 365,857 - GFF623 0.78 +0.3 365,857 - GFF623 0.78 -0.5 365,928 - GFF623 0.80 +0.3 365,934 - GFF623 0.81 -0.8 366,073 - GFF623 0.86 +0.5 366,073 - GFF623 0.86 +0.7 366,129 + GFF623 0.88 +1.0 366,159 - GFF623 0.89 +0.1 366,248 + +0.3 366,259 - -0.2 366,279 - -0.4 366,331 + -0.7 366,346 - +0.2 366,363 - +0.4 366,410 - +0.9 366,414 - +0.1 366,484 + +0.5 366,484 + +0.2 366,486 + +0.2 366,572 + +0.9 366,583 - -0.2 366,583 - -0.1 366,723 - GFF624 0.22 -0.2 366,723 - GFF624 0.22 +0.1 366,752 + GFF624 0.26 -0.3 366,766 + GFF624 0.28 +0.5 366,927 - GFF624 0.48 -0.1 367,041 - GFF624 0.63 -0.1 367,059 + GFF624 0.65 -1.0 367,062 - GFF624 0.66 -0.5 367,204 - GFF624 0.84 -0.1 367,322 + +1.9 367,322 + -0.1 367,323 - -0.0 367,371 + +0.2 367,372 - -0.2 367,376 - +0.2 367,534 + GFF625 0.38 +0.9 367,535 - GFF625 0.38 -0.4 367,619 + GFF625 0.54 -0.6 367,619 + GFF625 0.54 +0.3 367,620 - GFF625 0.54 +0.3 367,620 - GFF625 0.54 +0.5 367,677 + GFF625 0.64 -1.2 367,870 - +0.4 368,265 + GFF626 0.87 +0.4
Or see this region's nucleotide sequence