Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF558 and GFF559 overlap by 4 nucleotides GFF559 and GFF560 are separated by 12 nucleotides GFF560 and GFF561 are separated by 125 nucleotides
GFF558 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 288,512 to 289,582
GFF558
GFF559 - Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46), at 289,579 to 290,319
GFF559
GFF560 - FIG00638953: hypothetical protein, at 290,332 to 290,748
GFF560
GFF561 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 290,874 to 292,616
GFF561
Position (kb) 290
291 Strain fitness (log2 ratio) -1
0
1 at 289.334 kb on - strand, within GFF558 at 289.334 kb on - strand, within GFF558 at 289.363 kb on + strand, within GFF558 at 289.364 kb on - strand, within GFF558 at 289.364 kb on - strand, within GFF558 at 289.407 kb on + strand, within GFF558 at 289.408 kb on - strand, within GFF558 at 289.411 kb on + strand, within GFF558 at 289.412 kb on - strand, within GFF558 at 289.412 kb on - strand, within GFF558 at 289.514 kb on - strand at 289.514 kb on - strand at 289.579 kb on - strand at 289.584 kb on + strand at 289.914 kb on + strand, within GFF559 at 290.027 kb on + strand, within GFF559 at 290.271 kb on + strand at 290.318 kb on - strand at 290.520 kb on + strand, within GFF560 at 290.671 kb on - strand, within GFF560 at 290.677 kb on - strand, within GFF560 at 290.677 kb on - strand, within GFF560 at 290.688 kb on + strand, within GFF560 at 290.689 kb on - strand, within GFF560 at 290.735 kb on - strand at 290.735 kb on - strand at 290.784 kb on + strand at 290.784 kb on + strand at 290.785 kb on - strand at 290.785 kb on - strand at 290.785 kb on - strand at 290.835 kb on - strand at 290.845 kb on + strand at 290.845 kb on + strand at 290.845 kb on + strand at 290.845 kb on + strand at 290.846 kb on - strand at 290.846 kb on - strand at 290.846 kb on - strand at 290.846 kb on - strand at 291.066 kb on - strand, within GFF561 at 291.167 kb on + strand, within GFF561 at 291.167 kb on + strand, within GFF561 at 291.168 kb on - strand, within GFF561 at 291.245 kb on + strand, within GFF561 at 291.355 kb on - strand, within GFF561 at 291.534 kb on - strand, within GFF561 at 291.625 kb on + strand, within GFF561 at 291.681 kb on + strand, within GFF561 at 291.682 kb on - strand, within GFF561
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 289,334 - GFF558 0.77 +0.1 289,334 - GFF558 0.77 +0.2 289,363 + GFF558 0.79 +0.0 289,364 - GFF558 0.80 -0.1 289,364 - GFF558 0.80 -0.2 289,407 + GFF558 0.84 +0.4 289,408 - GFF558 0.84 -0.9 289,411 + GFF558 0.84 +0.3 289,412 - GFF558 0.84 +0.3 289,412 - GFF558 0.84 +0.2 289,514 - -0.3 289,514 - -1.4 289,579 - -0.5 289,584 + -0.6 289,914 + GFF559 0.45 +1.6 290,027 + GFF559 0.60 -0.5 290,271 + +0.4 290,318 - -0.1 290,520 + GFF560 0.45 -0.8 290,671 - GFF560 0.81 -0.0 290,677 - GFF560 0.83 -0.2 290,677 - GFF560 0.83 +0.4 290,688 + GFF560 0.85 +0.9 290,689 - GFF560 0.86 -0.2 290,735 - +0.7 290,735 - +0.3 290,784 + +1.0 290,784 + +0.1 290,785 - -0.4 290,785 - -0.4 290,785 - +0.3 290,835 - +0.3 290,845 + +1.0 290,845 + -0.3 290,845 + +0.4 290,845 + -1.3 290,846 - +0.2 290,846 - -0.1 290,846 - +1.2 290,846 - -0.5 291,066 - GFF561 0.11 -0.1 291,167 + GFF561 0.17 -0.0 291,167 + GFF561 0.17 +0.1 291,168 - GFF561 0.17 -0.7 291,245 + GFF561 0.21 +0.6 291,355 - GFF561 0.28 +0.3 291,534 - GFF561 0.38 -0.5 291,625 + GFF561 0.43 +0.8 291,681 + GFF561 0.46 +0.5 291,682 - GFF561 0.46 -0.4
Or see this region's nucleotide sequence