Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF550 and GFF551 overlap by 4 nucleotides GFF551 and GFF552 are separated by 1 nucleotides GFF552 and GFF553 are separated by 131 nucleotides GFF553 and GFF554 are separated by 107 nucleotides GFF554 and GFF555 are separated by 258 nucleotides
GFF550 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 281,668 to 282,945
GFF550
GFF551 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 282,942 to 283,709
GFF551
GFF552 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 283,711 to 284,424
GFF552
GFF553 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 284,556 to 284,777
GFF553
GFF554 - Death on curing protein, Doc toxin, at 284,885 to 285,142
GFF554
GFF555 - Glycerol-3-phosphate ABC transporter, periplasmic glycerol-3-phosphate-binding protein (TC 3.A.1.1.3), at 285,401 to 286,717
GFF555
Position (kb) 283
284
285 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 282.955 kb on + strand at 283.085 kb on + strand, within GFF551 at 283.104 kb on + strand, within GFF551 at 283.541 kb on + strand, within GFF551 at 283.580 kb on + strand, within GFF551 at 283.693 kb on - strand at 283.708 kb on - strand at 283.708 kb on - strand at 283.908 kb on + strand, within GFF552 at 283.917 kb on - strand, within GFF552 at 284.059 kb on - strand, within GFF552 at 284.072 kb on - strand, within GFF552 at 284.183 kb on + strand, within GFF552 at 284.184 kb on - strand, within GFF552 at 284.410 kb on + strand at 284.411 kb on - strand at 284.530 kb on - strand at 284.538 kb on - strand at 284.571 kb on + strand at 284.572 kb on - strand at 284.572 kb on - strand at 284.572 kb on - strand at 284.654 kb on + strand, within GFF553 at 284.781 kb on + strand at 284.788 kb on + strand at 284.806 kb on - strand at 284.884 kb on - strand at 285.009 kb on + strand, within GFF554 at 285.013 kb on + strand, within GFF554 at 285.013 kb on + strand, within GFF554 at 285.017 kb on - strand, within GFF554 at 285.107 kb on - strand, within GFF554 at 285.132 kb on + strand at 285.204 kb on + strand at 285.204 kb on + strand at 285.212 kb on + strand at 285.213 kb on - strand at 285.216 kb on + strand at 285.284 kb on - strand at 285.395 kb on + strand at 285.395 kb on + strand at 285.418 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 282,955 + +1.5 283,085 + GFF551 0.19 +0.4 283,104 + GFF551 0.21 +0.4 283,541 + GFF551 0.78 -0.5 283,580 + GFF551 0.83 +0.3 283,693 - -0.3 283,708 - +0.5 283,708 - -0.9 283,908 + GFF552 0.28 -0.4 283,917 - GFF552 0.29 +0.3 284,059 - GFF552 0.49 -0.6 284,072 - GFF552 0.51 +0.1 284,183 + GFF552 0.66 -0.3 284,184 - GFF552 0.66 +0.2 284,410 + +0.6 284,411 - -0.6 284,530 - -0.8 284,538 - +0.1 284,571 + +0.8 284,572 - +4.1 284,572 - +0.6 284,572 - +0.5 284,654 + GFF553 0.44 -0.0 284,781 + +0.4 284,788 + +0.2 284,806 - +0.2 284,884 - -0.0 285,009 + GFF554 0.48 +0.2 285,013 + GFF554 0.50 +0.1 285,013 + GFF554 0.50 +0.1 285,017 - GFF554 0.51 -0.7 285,107 - GFF554 0.86 -0.1 285,132 + +0.7 285,204 + -0.8 285,204 + +0.7 285,212 + +0.6 285,213 - +0.2 285,216 + +0.2 285,284 - +0.2 285,395 + +0.6 285,395 + -0.8 285,418 + +0.1
Or see this region's nucleotide sequence