Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF530 and GFF531 are separated by 131 nucleotides GFF531 and GFF532 are separated by 3 nucleotides GFF532 and GFF533 are separated by 72 nucleotides
GFF530 - Putative PerM family permease, at 262,457 to 263,506
GFF530
GFF531 - Transporter, putative, at 263,638 to 264,855
GFF531
GFF532 - DcrB protein precursor, at 264,859 to 265,416
GFF532
GFF533 - Putative preQ0 transporter, at 265,489 to 266,229
GFF533
Position (kb) 263
264
265 Strain fitness (log2 ratio) -1
0
1
2
3 at 262.746 kb on - strand, within GFF530 at 262.795 kb on + strand, within GFF530 at 262.795 kb on + strand, within GFF530 at 262.804 kb on - strand, within GFF530 at 262.804 kb on - strand, within GFF530 at 262.823 kb on + strand, within GFF530 at 262.877 kb on - strand, within GFF530 at 262.892 kb on + strand, within GFF530 at 262.921 kb on - strand, within GFF530 at 262.996 kb on + strand, within GFF530 at 263.087 kb on + strand, within GFF530 at 263.088 kb on - strand, within GFF530 at 263.138 kb on - strand, within GFF530 at 263.152 kb on + strand, within GFF530 at 263.152 kb on + strand, within GFF530 at 263.156 kb on + strand, within GFF530 at 263.230 kb on + strand, within GFF530 at 263.237 kb on - strand, within GFF530 at 263.279 kb on - strand, within GFF530 at 263.312 kb on - strand, within GFF530 at 263.408 kb on - strand at 263.441 kb on - strand at 263.600 kb on + strand at 263.620 kb on - strand at 263.634 kb on - strand at 263.692 kb on - strand at 263.692 kb on - strand at 263.712 kb on + strand at 263.712 kb on + strand at 263.725 kb on + strand at 263.808 kb on + strand, within GFF531 at 263.809 kb on - strand, within GFF531 at 263.824 kb on + strand, within GFF531 at 263.825 kb on - strand, within GFF531 at 263.840 kb on + strand, within GFF531 at 263.864 kb on - strand, within GFF531 at 263.874 kb on - strand, within GFF531 at 263.874 kb on - strand, within GFF531 at 264.157 kb on + strand, within GFF531 at 264.225 kb on - strand, within GFF531 at 264.304 kb on + strand, within GFF531 at 264.358 kb on - strand, within GFF531 at 264.360 kb on + strand, within GFF531 at 264.378 kb on + strand, within GFF531 at 264.670 kb on + strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.671 kb on - strand, within GFF531 at 264.864 kb on + strand at 264.865 kb on - strand at 264.876 kb on + strand at 264.877 kb on - strand at 265.035 kb on + strand, within GFF532 at 265.053 kb on + strand, within GFF532 at 265.054 kb on - strand, within GFF532 at 265.059 kb on + strand, within GFF532 at 265.060 kb on - strand, within GFF532 at 265.060 kb on - strand, within GFF532 at 265.093 kb on - strand, within GFF532 at 265.206 kb on + strand, within GFF532 at 265.344 kb on + strand, within GFF532 at 265.397 kb on + strand at 265.397 kb on + strand at 265.522 kb on - strand at 265.607 kb on + strand, within GFF533 at 265.644 kb on + strand, within GFF533 at 265.807 kb on - strand, within GFF533
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 262,746 - GFF530 0.28 +0.4 262,795 + GFF530 0.32 -0.7 262,795 + GFF530 0.32 +0.4 262,804 - GFF530 0.33 -0.4 262,804 - GFF530 0.33 +0.1 262,823 + GFF530 0.35 +0.4 262,877 - GFF530 0.40 +0.9 262,892 + GFF530 0.41 -0.0 262,921 - GFF530 0.44 -0.7 262,996 + GFF530 0.51 +0.0 263,087 + GFF530 0.60 -1.0 263,088 - GFF530 0.60 -0.2 263,138 - GFF530 0.65 +0.2 263,152 + GFF530 0.66 +0.7 263,152 + GFF530 0.66 +0.1 263,156 + GFF530 0.67 +1.8 263,230 + GFF530 0.74 +0.1 263,237 - GFF530 0.74 -0.0 263,279 - GFF530 0.78 -0.3 263,312 - GFF530 0.81 +1.0 263,408 - +0.9 263,441 - -0.3 263,600 + -0.1 263,620 - +0.4 263,634 - -0.5 263,692 - +1.5 263,692 - -0.3 263,712 + +0.2 263,712 + +0.2 263,725 + -0.2 263,808 + GFF531 0.14 +0.1 263,809 - GFF531 0.14 -0.3 263,824 + GFF531 0.15 +0.3 263,825 - GFF531 0.15 -0.5 263,840 + GFF531 0.17 -0.1 263,864 - GFF531 0.19 -0.3 263,874 - GFF531 0.19 +0.2 263,874 - GFF531 0.19 +0.1 264,157 + GFF531 0.43 +0.3 264,225 - GFF531 0.48 +0.0 264,304 + GFF531 0.55 -0.2 264,358 - GFF531 0.59 -0.1 264,360 + GFF531 0.59 +0.2 264,378 + GFF531 0.61 -0.3 264,670 + GFF531 0.85 +0.5 264,671 - GFF531 0.85 +3.4 264,671 - GFF531 0.85 +0.6 264,671 - GFF531 0.85 +0.3 264,671 - GFF531 0.85 +3.0 264,864 + +0.3 264,865 - +0.6 264,876 + +0.3 264,877 - +0.5 265,035 + GFF532 0.32 +1.2 265,053 + GFF532 0.35 +1.7 265,054 - GFF532 0.35 +0.3 265,059 + GFF532 0.36 -0.8 265,060 - GFF532 0.36 +0.9 265,060 - GFF532 0.36 +1.0 265,093 - GFF532 0.42 +0.9 265,206 + GFF532 0.62 +1.5 265,344 + GFF532 0.87 +0.0 265,397 + +0.4 265,397 + +0.6 265,522 - +0.1 265,607 + GFF533 0.16 +0.6 265,644 + GFF533 0.21 +0.2 265,807 - GFF533 0.43 -0.8
Or see this region's nucleotide sequence