Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4869 and GFF4870 are separated by 51 nucleotides GFF4870 and GFF4871 are separated by 109 nucleotides
GFF4869 - FIG00761799: membrane protein, at 167,842 to 168,756
GFF4869
GFF4870 - CsdL (EC-YgdL) protein of the HesA/MoeB/ThiF family, part of the CsdA-E-L sulfur transfer pathway, at 168,808 to 169,614
GFF4870
GFF4871 - Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-), at 169,724 to 170,821
GFF4871
Position (kb) 168
169
170 Strain fitness (log2 ratio) -2
-1
0
1 at 168.302 kb on + strand, within GFF4869 at 168.351 kb on - strand, within GFF4869 at 168.351 kb on - strand, within GFF4869 at 168.510 kb on - strand, within GFF4869 at 168.672 kb on - strand at 169.024 kb on - strand, within GFF4870 at 169.053 kb on + strand, within GFF4870 at 169.169 kb on - strand, within GFF4870 at 169.180 kb on - strand, within GFF4870 at 169.181 kb on + strand, within GFF4870 at 169.244 kb on + strand, within GFF4870 at 169.244 kb on + strand, within GFF4870 at 169.245 kb on - strand, within GFF4870 at 169.249 kb on - strand, within GFF4870 at 169.601 kb on - strand at 169.601 kb on - strand at 169.614 kb on + strand at 169.615 kb on - strand at 169.791 kb on + strand at 169.791 kb on + strand at 169.892 kb on + strand, within GFF4871 at 169.899 kb on + strand, within GFF4871 at 169.900 kb on - strand, within GFF4871 at 169.938 kb on - strand, within GFF4871 at 170.130 kb on - strand, within GFF4871 at 170.227 kb on + strand, within GFF4871 at 170.265 kb on + strand, within GFF4871 at 170.290 kb on + strand, within GFF4871 at 170.328 kb on + strand, within GFF4871 at 170.328 kb on + strand, within GFF4871 at 170.328 kb on + strand, within GFF4871 at 170.329 kb on - strand, within GFF4871 at 170.332 kb on + strand, within GFF4871 at 170.398 kb on + strand, within GFF4871 at 170.398 kb on + strand, within GFF4871 at 170.400 kb on + strand, within GFF4871 at 170.400 kb on + strand, within GFF4871 at 170.400 kb on + strand, within GFF4871 at 170.401 kb on - strand, within GFF4871 at 170.405 kb on - strand, within GFF4871 at 170.455 kb on + strand, within GFF4871 at 170.456 kb on - strand, within GFF4871 at 170.456 kb on - strand, within GFF4871 at 170.460 kb on + strand, within GFF4871 at 170.533 kb on + strand, within GFF4871 at 170.579 kb on - strand, within GFF4871 at 170.581 kb on - strand, within GFF4871 at 170.601 kb on + strand, within GFF4871 at 170.603 kb on + strand, within GFF4871
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 168,302 + GFF4869 0.50 +0.2 168,351 - GFF4869 0.56 -0.4 168,351 - GFF4869 0.56 -0.1 168,510 - GFF4869 0.73 +0.6 168,672 - +0.0 169,024 - GFF4870 0.27 +0.0 169,053 + GFF4870 0.30 -0.3 169,169 - GFF4870 0.45 -0.5 169,180 - GFF4870 0.46 -0.2 169,181 + GFF4870 0.46 -0.5 169,244 + GFF4870 0.54 +0.3 169,244 + GFF4870 0.54 +0.0 169,245 - GFF4870 0.54 -0.2 169,249 - GFF4870 0.55 -0.6 169,601 - -0.4 169,601 - +0.1 169,614 + +0.1 169,615 - -0.1 169,791 + -0.4 169,791 + +0.2 169,892 + GFF4871 0.15 -0.0 169,899 + GFF4871 0.16 -1.8 169,900 - GFF4871 0.16 -0.1 169,938 - GFF4871 0.19 -1.0 170,130 - GFF4871 0.37 -0.1 170,227 + GFF4871 0.46 +0.2 170,265 + GFF4871 0.49 -1.3 170,290 + GFF4871 0.52 -0.1 170,328 + GFF4871 0.55 +1.1 170,328 + GFF4871 0.55 -0.2 170,328 + GFF4871 0.55 -0.1 170,329 - GFF4871 0.55 -0.2 170,332 + GFF4871 0.55 -0.4 170,398 + GFF4871 0.61 +0.3 170,398 + GFF4871 0.61 +0.0 170,400 + GFF4871 0.62 +0.4 170,400 + GFF4871 0.62 -0.9 170,400 + GFF4871 0.62 -0.3 170,401 - GFF4871 0.62 -0.1 170,405 - GFF4871 0.62 -0.4 170,455 + GFF4871 0.67 -0.0 170,456 - GFF4871 0.67 -0.9 170,456 - GFF4871 0.67 +1.1 170,460 + GFF4871 0.67 +0.5 170,533 + GFF4871 0.74 -0.3 170,579 - GFF4871 0.78 +0.5 170,581 - GFF4871 0.78 +0.3 170,601 + GFF4871 0.80 -0.0 170,603 + GFF4871 0.80 -1.0
Or see this region's nucleotide sequence