Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4853 and GFF4854 are separated by 170 nucleotides GFF4854 and GFF4855 are separated by 91 nucleotides
GFF4853 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 152,085 to 153,452
GFF4853
GFF4854 - DNA polymerase I (EC 2.7.7.7), at 153,623 to 154,378
GFF4854
GFF4855 - Lactaldehyde reductase (EC 1.1.1.77), at 154,470 to 155,618
GFF4855
Position (kb) 153
154
155 Strain fitness (log2 ratio) -1
0
1 at 152.879 kb on + strand, within GFF4853 at 152.926 kb on + strand, within GFF4853 at 152.942 kb on + strand, within GFF4853 at 152.958 kb on - strand, within GFF4853 at 152.958 kb on - strand, within GFF4853 at 152.980 kb on - strand, within GFF4853 at 152.980 kb on - strand, within GFF4853 at 152.983 kb on - strand, within GFF4853 at 153.028 kb on - strand, within GFF4853 at 153.030 kb on - strand, within GFF4853 at 153.061 kb on - strand, within GFF4853 at 153.209 kb on + strand, within GFF4853 at 153.209 kb on + strand, within GFF4853 at 153.248 kb on - strand, within GFF4853 at 153.324 kb on - strand at 153.340 kb on + strand at 153.340 kb on + strand at 153.395 kb on + strand at 153.542 kb on + strand at 153.555 kb on - strand at 153.591 kb on - strand at 153.734 kb on - strand, within GFF4854 at 153.852 kb on + strand, within GFF4854 at 153.856 kb on + strand, within GFF4854 at 153.857 kb on - strand, within GFF4854 at 154.011 kb on - strand, within GFF4854 at 154.102 kb on - strand, within GFF4854 at 154.148 kb on - strand, within GFF4854 at 154.148 kb on - strand, within GFF4854 at 154.336 kb on - strand at 154.362 kb on + strand at 154.471 kb on + strand at 154.487 kb on - strand at 154.637 kb on + strand, within GFF4855 at 154.661 kb on + strand, within GFF4855 at 154.880 kb on - strand, within GFF4855 at 154.967 kb on + strand, within GFF4855 at 154.968 kb on - strand, within GFF4855 at 154.971 kb on + strand, within GFF4855 at 154.971 kb on + strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 155.007 kb on - strand, within GFF4855 at 155.007 kb on - strand, within GFF4855 at 155.009 kb on - strand, within GFF4855 at 155.018 kb on + strand, within GFF4855 at 155.167 kb on + strand, within GFF4855 at 155.217 kb on - strand, within GFF4855 at 155.288 kb on + strand, within GFF4855 at 155.351 kb on - strand, within GFF4855
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 152,879 + GFF4853 0.58 +0.2 152,926 + GFF4853 0.61 -0.1 152,942 + GFF4853 0.63 +0.1 152,958 - GFF4853 0.64 +0.6 152,958 - GFF4853 0.64 -1.1 152,980 - GFF4853 0.65 -0.7 152,980 - GFF4853 0.65 -0.1 152,983 - GFF4853 0.66 +0.4 153,028 - GFF4853 0.69 +0.6 153,030 - GFF4853 0.69 +0.5 153,061 - GFF4853 0.71 -0.5 153,209 + GFF4853 0.82 +0.4 153,209 + GFF4853 0.82 -1.3 153,248 - GFF4853 0.85 +0.2 153,324 - -0.3 153,340 + +0.9 153,340 + -0.1 153,395 + +0.1 153,542 + +0.6 153,555 - +0.2 153,591 - +0.3 153,734 - GFF4854 0.15 +0.1 153,852 + GFF4854 0.30 -0.3 153,856 + GFF4854 0.31 +1.1 153,857 - GFF4854 0.31 +0.6 154,011 - GFF4854 0.51 +0.7 154,102 - GFF4854 0.63 -0.4 154,148 - GFF4854 0.69 -0.7 154,148 - GFF4854 0.69 -0.1 154,336 - +0.8 154,362 + +0.1 154,471 + -1.7 154,487 - -0.7 154,637 + GFF4855 0.15 -0.8 154,661 + GFF4855 0.17 -0.6 154,880 - GFF4855 0.36 -0.3 154,967 + GFF4855 0.43 +0.4 154,968 - GFF4855 0.43 +0.4 154,971 + GFF4855 0.44 -0.0 154,971 + GFF4855 0.44 +0.2 154,972 - GFF4855 0.44 -0.4 154,972 - GFF4855 0.44 -0.5 154,972 - GFF4855 0.44 +0.3 154,972 - GFF4855 0.44 -0.6 154,972 - GFF4855 0.44 +0.1 155,007 - GFF4855 0.47 +0.4 155,007 - GFF4855 0.47 -0.3 155,009 - GFF4855 0.47 -0.9 155,018 + GFF4855 0.48 -0.4 155,167 + GFF4855 0.61 +0.5 155,217 - GFF4855 0.65 +0.1 155,288 + GFF4855 0.71 +1.3 155,351 - GFF4855 0.77 -0.1
Or see this region's nucleotide sequence