Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF477 and GFF478 are separated by 10 nucleotides GFF478 and GFF479 overlap by 4 nucleotides GFF479 and GFF480 are separated by 100 nucleotides
GFF477 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 199,842 to 200,744
GFF477
GFF478 - Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2), at 200,755 to 201,738
GFF478
GFF479 - Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2), at 201,735 to 202,748
GFF479
GFF480 - Serine transporter, at 202,849 to 204,147
GFF480
Position (kb) 201
202
203 Strain fitness (log2 ratio) -1
0
1 at 200.743 kb on - strand at 200.765 kb on + strand at 200.833 kb on - strand at 200.833 kb on - strand at 200.837 kb on - strand at 200.837 kb on - strand at 200.913 kb on + strand, within GFF478 at 201.070 kb on + strand, within GFF478 at 201.070 kb on + strand, within GFF478 at 201.071 kb on - strand, within GFF478 at 201.072 kb on + strand, within GFF478 at 201.072 kb on + strand, within GFF478 at 201.384 kb on + strand, within GFF478 at 201.500 kb on - strand, within GFF478 at 201.500 kb on - strand, within GFF478 at 201.500 kb on - strand, within GFF478 at 201.587 kb on - strand, within GFF478 at 201.734 kb on + strand at 201.735 kb on - strand at 201.740 kb on + strand at 201.740 kb on + strand at 201.973 kb on - strand, within GFF479 at 201.976 kb on - strand, within GFF479 at 201.996 kb on - strand, within GFF479 at 202.122 kb on - strand, within GFF479 at 202.377 kb on + strand, within GFF479 at 202.377 kb on + strand, within GFF479 at 202.378 kb on - strand, within GFF479 at 202.378 kb on - strand, within GFF479 at 202.385 kb on + strand, within GFF479 at 202.385 kb on + strand, within GFF479 at 202.440 kb on + strand, within GFF479 at 202.440 kb on + strand, within GFF479 at 202.441 kb on - strand, within GFF479 at 202.444 kb on + strand, within GFF479 at 202.503 kb on + strand, within GFF479 at 202.652 kb on + strand at 202.674 kb on - strand at 202.790 kb on - strand at 202.895 kb on + strand at 202.974 kb on + strand at 203.005 kb on + strand, within GFF480 at 203.229 kb on + strand, within GFF480 at 203.230 kb on - strand, within GFF480 at 203.254 kb on + strand, within GFF480 at 203.374 kb on - strand, within GFF480 at 203.375 kb on + strand, within GFF480 at 203.376 kb on - strand, within GFF480
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 200,743 - +0.1 200,765 + +0.5 200,833 - +0.8 200,833 - -0.7 200,837 - -0.1 200,837 - -0.3 200,913 + GFF478 0.16 +0.8 201,070 + GFF478 0.32 +1.0 201,070 + GFF478 0.32 -0.2 201,071 - GFF478 0.32 +1.4 201,072 + GFF478 0.32 -0.3 201,072 + GFF478 0.32 -0.3 201,384 + GFF478 0.64 +1.6 201,500 - GFF478 0.76 +0.1 201,500 - GFF478 0.76 +0.8 201,500 - GFF478 0.76 +0.3 201,587 - GFF478 0.85 +0.4 201,734 + -0.8 201,735 - +0.4 201,740 + +0.4 201,740 + -0.4 201,973 - GFF479 0.23 +0.0 201,976 - GFF479 0.24 -0.8 201,996 - GFF479 0.26 -0.4 202,122 - GFF479 0.38 -0.0 202,377 + GFF479 0.63 -1.1 202,377 + GFF479 0.63 +0.6 202,378 - GFF479 0.63 -0.6 202,378 - GFF479 0.63 -0.5 202,385 + GFF479 0.64 -0.6 202,385 + GFF479 0.64 -0.5 202,440 + GFF479 0.70 -0.5 202,440 + GFF479 0.70 +0.5 202,441 - GFF479 0.70 -1.4 202,444 + GFF479 0.70 -0.1 202,503 + GFF479 0.76 +0.6 202,652 + +1.1 202,674 - -0.7 202,790 - +0.4 202,895 + -0.4 202,974 + -0.2 203,005 + GFF480 0.12 -0.3 203,229 + GFF480 0.29 +0.2 203,230 - GFF480 0.29 -0.0 203,254 + GFF480 0.31 +0.0 203,374 - GFF480 0.40 -0.4 203,375 + GFF480 0.40 +0.3 203,376 - GFF480 0.41 -0.1
Or see this region's nucleotide sequence