Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4642 and GFF4643 are separated by 28 nucleotides GFF4643 and GFF4644 are separated by 86 nucleotides GFF4644 and GFF4645 are separated by 621 nucleotides GFF4645 and GFF4646 are separated by 469 nucleotides
GFF4642 - Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5), at 437,621 to 438,490
GFF4642
GFF4643 - hypothetical protein, at 438,519 to 438,644
GFF4643
GFF4644 - hypothetical protein, at 438,731 to 438,856
GFF4644
GFF4645 - hypothetical protein, at 439,478 to 439,603
GFF4645
GFF4646 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 440,073 to 441,641
GFF4646
Position (kb) 439
440 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 438.572 kb on + strand, within GFF4643 at 438.807 kb on - strand, within GFF4644 at 438.850 kb on - strand at 440.077 kb on + strand at 440.081 kb on + strand at 440.081 kb on + strand at 440.082 kb on - strand at 440.082 kb on - strand at 440.084 kb on - strand at 440.101 kb on + strand at 440.191 kb on - strand at 440.191 kb on - strand at 440.191 kb on - strand at 440.216 kb on + strand at 440.332 kb on - strand, within GFF4646 at 440.405 kb on - strand, within GFF4646 at 440.405 kb on - strand, within GFF4646 at 440.438 kb on + strand, within GFF4646 at 440.450 kb on + strand, within GFF4646 at 440.451 kb on - strand, within GFF4646
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 438,572 + GFF4643 0.42 +0.5 438,807 - GFF4644 0.60 +0.7 438,850 - -0.4 440,077 + -1.4 440,081 + -0.2 440,081 + -1.5 440,082 - -1.9 440,082 - -2.8 440,084 - -3.7 440,101 + -2.0 440,191 - -2.5 440,191 - -0.2 440,191 - -2.1 440,216 + -1.1 440,332 - GFF4646 0.17 -1.8 440,405 - GFF4646 0.21 -2.0 440,405 - GFF4646 0.21 -1.1 440,438 + GFF4646 0.23 -1.5 440,450 + GFF4646 0.24 -1.8 440,451 - GFF4646 0.24 -1.4
Or see this region's nucleotide sequence