Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4237 and GFF4238 are separated by 99 nucleotides GFF4238 and GFF4239 overlap by 28 nucleotides GFF4239 and GFF4240 are separated by 121 nucleotides GFF4240 and GFF4241 are separated by 141 nucleotides
GFF4237 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 17,345 to 18,895
GFF4237
GFF4238 - Possible transmembrane regulator, at 18,995 to 19,738
GFF4238
GFF4239 - Putative inner membrane protein, at 19,711 to 20,199
GFF4239
GFF4240 - hypothetical protein, at 20,321 to 20,458
GFF4240
GFF4241 - Attachment invasion locus protein precursor, at 20,600 to 21,124
GFF4241
Position (kb) 18
19
20 Strain fitness (log2 ratio) -1
0
1 at 18.141 kb on - strand, within GFF4237 at 18.271 kb on + strand, within GFF4237 at 18.377 kb on - strand, within GFF4237 at 18.391 kb on + strand, within GFF4237 at 18.393 kb on + strand, within GFF4237 at 18.665 kb on - strand, within GFF4237 at 18.670 kb on + strand, within GFF4237 at 18.671 kb on - strand, within GFF4237 at 18.738 kb on - strand, within GFF4237 at 18.744 kb on + strand at 18.745 kb on - strand at 18.808 kb on + strand at 20.087 kb on + strand, within GFF4239 at 20.131 kb on - strand, within GFF4239 at 20.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 18,141 - GFF4237 0.51 -0.4 18,271 + GFF4237 0.60 -0.0 18,377 - GFF4237 0.67 +0.0 18,391 + GFF4237 0.67 -0.5 18,393 + GFF4237 0.68 -0.2 18,665 - GFF4237 0.85 -0.5 18,670 + GFF4237 0.85 -0.4 18,671 - GFF4237 0.85 -0.8 18,738 - GFF4237 0.90 -0.4 18,744 + -0.5 18,745 - -0.2 18,808 + -0.4 20,087 + GFF4239 0.77 -0.4 20,131 - GFF4239 0.86 +0.4 20,197 + -0.0
Or see this region's nucleotide sequence