Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4177 and GFF4178 are separated by 57 nucleotides GFF4178 and GFF4179 overlap by 40 nucleotides GFF4179 and GFF4180 are separated by 250 nucleotides GFF4180 and GFF4181 are separated by 271 nucleotides
GFF4177 - Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43), at 66,032 to 67,153
GFF4177
GFF4178 - FIG074102: hypothetical protein, at 67,211 to 68,119
GFF4178
GFF4179 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 68,080 to 69,240
GFF4179
GFF4180 - Ribosome hibernation protein YfiA, at 69,491 to 69,829
GFF4180
GFF4181 - Probable component of the lipoprotein assembly complex (forms a complex with YaeT, YfgL, and NlpB), at 70,101 to 70,838
GFF4181
Position (kb) 68
69
70 Strain fitness (log2 ratio) -1
0
1
2 at 67.165 kb on - strand at 67.167 kb on - strand at 67.167 kb on - strand at 67.170 kb on - strand at 67.216 kb on + strand at 67.230 kb on + strand at 67.292 kb on + strand at 67.351 kb on + strand, within GFF4178 at 67.352 kb on - strand, within GFF4178 at 67.432 kb on + strand, within GFF4178 at 67.432 kb on + strand, within GFF4178 at 67.447 kb on + strand, within GFF4178 at 67.458 kb on + strand, within GFF4178 at 67.492 kb on + strand, within GFF4178 at 67.574 kb on + strand, within GFF4178 at 67.575 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.687 kb on + strand, within GFF4178 at 67.713 kb on + strand, within GFF4178 at 67.715 kb on + strand, within GFF4178 at 67.715 kb on + strand, within GFF4178 at 67.716 kb on - strand, within GFF4178 at 67.716 kb on - strand, within GFF4178 at 67.826 kb on + strand, within GFF4178 at 67.960 kb on + strand, within GFF4178 at 67.969 kb on + strand, within GFF4178 at 67.969 kb on + strand, within GFF4178 at 68.179 kb on + strand at 68.213 kb on + strand, within GFF4179 at 68.413 kb on - strand, within GFF4179 at 68.541 kb on + strand, within GFF4179 at 68.551 kb on - strand, within GFF4179 at 68.552 kb on + strand, within GFF4179 at 68.621 kb on + strand, within GFF4179 at 68.621 kb on + strand, within GFF4179 at 68.725 kb on + strand, within GFF4179 at 68.784 kb on - strand, within GFF4179 at 68.847 kb on + strand, within GFF4179 at 68.925 kb on + strand, within GFF4179 at 68.926 kb on - strand, within GFF4179 at 68.926 kb on - strand, within GFF4179 at 69.026 kb on + strand, within GFF4179 at 69.026 kb on + strand, within GFF4179 at 69.027 kb on - strand, within GFF4179 at 69.122 kb on - strand, within GFF4179 at 69.170 kb on - strand at 69.315 kb on + strand at 69.352 kb on - strand at 69.387 kb on + strand at 69.408 kb on + strand at 69.485 kb on - strand at 69.493 kb on - strand at 69.571 kb on + strand, within GFF4180 at 69.618 kb on + strand, within GFF4180 at 69.619 kb on - strand, within GFF4180 at 69.619 kb on - strand, within GFF4180 at 69.622 kb on + strand, within GFF4180 at 69.622 kb on + strand, within GFF4180 at 69.623 kb on - strand, within GFF4180 at 69.664 kb on + strand, within GFF4180 at 69.703 kb on + strand, within GFF4180 at 69.715 kb on + strand, within GFF4180 at 69.881 kb on - strand at 70.001 kb on - strand at 70.001 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 67,165 - +1.5 67,167 - -0.3 67,167 - -0.7 67,170 - +0.1 67,216 + -0.3 67,230 + +0.3 67,292 + -0.3 67,351 + GFF4178 0.15 +0.3 67,352 - GFF4178 0.16 -0.1 67,432 + GFF4178 0.24 +0.3 67,432 + GFF4178 0.24 +0.4 67,447 + GFF4178 0.26 +0.8 67,458 + GFF4178 0.27 +0.5 67,492 + GFF4178 0.31 +0.5 67,574 + GFF4178 0.40 -0.4 67,575 - GFF4178 0.40 +0.3 67,686 - GFF4178 0.52 +0.3 67,686 - GFF4178 0.52 +1.0 67,686 - GFF4178 0.52 +0.5 67,687 + GFF4178 0.52 -0.2 67,713 + GFF4178 0.55 +0.1 67,715 + GFF4178 0.55 +0.2 67,715 + GFF4178 0.55 +0.9 67,716 - GFF4178 0.56 +0.1 67,716 - GFF4178 0.56 +0.3 67,826 + GFF4178 0.68 +0.6 67,960 + GFF4178 0.82 +0.5 67,969 + GFF4178 0.83 -0.4 67,969 + GFF4178 0.83 -0.3 68,179 + -0.0 68,213 + GFF4179 0.11 +0.3 68,413 - GFF4179 0.29 +1.4 68,541 + GFF4179 0.40 +0.5 68,551 - GFF4179 0.41 +0.3 68,552 + GFF4179 0.41 +1.0 68,621 + GFF4179 0.47 +0.1 68,621 + GFF4179 0.47 +1.1 68,725 + GFF4179 0.56 +0.4 68,784 - GFF4179 0.61 +0.4 68,847 + GFF4179 0.66 -0.3 68,925 + GFF4179 0.73 +0.7 68,926 - GFF4179 0.73 +0.0 68,926 - GFF4179 0.73 -0.9 69,026 + GFF4179 0.81 +2.3 69,026 + GFF4179 0.81 +1.0 69,027 - GFF4179 0.82 -1.2 69,122 - GFF4179 0.90 -0.4 69,170 - -0.2 69,315 + -0.1 69,352 - +0.2 69,387 + -0.1 69,408 + -0.1 69,485 - +0.1 69,493 - +1.2 69,571 + GFF4180 0.24 +0.3 69,618 + GFF4180 0.37 +0.3 69,619 - GFF4180 0.38 -0.3 69,619 - GFF4180 0.38 -1.4 69,622 + GFF4180 0.39 -1.6 69,622 + GFF4180 0.39 +0.1 69,623 - GFF4180 0.39 -0.6 69,664 + GFF4180 0.51 -0.5 69,703 + GFF4180 0.63 +0.3 69,715 + GFF4180 0.66 -0.3 69,881 - +0.2 70,001 - +1.0 70,001 - -0.1
Or see this region's nucleotide sequence