Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3857 and GFF3858 are separated by 133 nucleotides GFF3858 and GFF3859 overlap by 33 nucleotides
GFF3857 - Putative heat shock protein YegD, at 131,009 to 132,361
GFF3857
GFF3858 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 132,495 to 133,364
GFF3858
GFF3859 - FIG00509877: hypothetical protein, at 133,332 to 136,328
GFF3859
Position (kb) 132
133
134 Strain fitness (log2 ratio) -1
0
1 at 131.578 kb on + strand, within GFF3857 at 131.953 kb on + strand, within GFF3857 at 131.953 kb on + strand, within GFF3857 at 131.954 kb on - strand, within GFF3857 at 131.957 kb on + strand, within GFF3857 at 131.957 kb on + strand, within GFF3857 at 131.958 kb on - strand, within GFF3857 at 131.958 kb on - strand, within GFF3857 at 132.127 kb on + strand, within GFF3857 at 132.144 kb on + strand, within GFF3857 at 132.144 kb on + strand, within GFF3857 at 132.253 kb on + strand at 132.253 kb on + strand at 132.254 kb on - strand at 132.274 kb on + strand at 132.274 kb on + strand at 132.365 kb on - strand at 132.465 kb on - strand at 132.522 kb on + strand at 132.694 kb on + strand, within GFF3858 at 132.830 kb on + strand, within GFF3858 at 132.830 kb on + strand, within GFF3858 at 133.217 kb on + strand, within GFF3858 at 133.291 kb on - strand at 133.348 kb on + strand at 133.422 kb on - strand at 133.576 kb on + strand at 133.600 kb on - strand at 133.731 kb on + strand, within GFF3859 at 133.731 kb on + strand, within GFF3859 at 133.732 kb on - strand, within GFF3859 at 133.799 kb on - strand, within GFF3859 at 134.069 kb on + strand, within GFF3859 at 134.069 kb on + strand, within GFF3859 at 134.069 kb on + strand, within GFF3859 at 134.216 kb on + strand, within GFF3859 at 134.217 kb on - strand, within GFF3859 at 134.217 kb on - strand, within GFF3859 at 134.227 kb on - strand, within GFF3859 at 134.307 kb on - strand, within GFF3859 at 134.340 kb on - strand, within GFF3859 at 134.343 kb on - strand, within GFF3859
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 131,578 + GFF3857 0.42 -0.0 131,953 + GFF3857 0.70 -0.2 131,953 + GFF3857 0.70 +0.2 131,954 - GFF3857 0.70 -0.3 131,957 + GFF3857 0.70 -0.0 131,957 + GFF3857 0.70 -0.4 131,958 - GFF3857 0.70 -0.1 131,958 - GFF3857 0.70 +0.1 132,127 + GFF3857 0.83 +0.5 132,144 + GFF3857 0.84 -0.8 132,144 + GFF3857 0.84 -0.2 132,253 + -0.6 132,253 + -0.0 132,254 - -0.4 132,274 + -0.2 132,274 + +0.1 132,365 - -0.3 132,465 - +0.3 132,522 + -0.2 132,694 + GFF3858 0.23 +0.7 132,830 + GFF3858 0.39 +0.3 132,830 + GFF3858 0.39 +1.2 133,217 + GFF3858 0.83 +0.7 133,291 - +0.7 133,348 + -0.2 133,422 - -0.2 133,576 + -0.0 133,600 - +0.0 133,731 + GFF3859 0.13 +0.2 133,731 + GFF3859 0.13 +0.3 133,732 - GFF3859 0.13 -0.0 133,799 - GFF3859 0.16 +0.2 134,069 + GFF3859 0.25 -1.1 134,069 + GFF3859 0.25 +0.5 134,069 + GFF3859 0.25 -0.0 134,216 + GFF3859 0.29 -1.0 134,217 - GFF3859 0.30 +0.0 134,217 - GFF3859 0.30 -0.4 134,227 - GFF3859 0.30 -0.6 134,307 - GFF3859 0.33 -0.2 134,340 - GFF3859 0.34 -0.1 134,343 - GFF3859 0.34 +0.1
Or see this region's nucleotide sequence