Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3801 and GFF3802 are separated by 8 nucleotides GFF3802 and GFF3803 are separated by 14 nucleotides GFF3803 and GFF3804 are separated by 51 nucleotides
GFF3801 - Fumarylacetoacetase (EC 3.7.1.2), at 70,519 to 71,220
GFF3801
GFF3802 - Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18), at 71,229 to 71,873
GFF3802
GFF3803 - Putative n-hydroxybenzoate hydroxylase, at 71,888 to 73,081
GFF3803
GFF3804 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 73,133 to 74,128
GFF3804
Position (kb)
71
72
73
74 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 70.959 kb on - strand, within GFF3801 at 70.981 kb on - strand, within GFF3801 at 70.999 kb on - strand, within GFF3801 at 71.080 kb on + strand, within GFF3801 at 71.184 kb on - strand at 71.184 kb on - strand at 71.272 kb on + strand at 71.277 kb on - strand at 71.538 kb on - strand, within GFF3802 at 71.539 kb on + strand, within GFF3802 at 71.970 kb on + strand at 72.112 kb on + strand, within GFF3803 at 72.113 kb on - strand, within GFF3803 at 72.217 kb on + strand, within GFF3803 at 72.218 kb on - strand, within GFF3803 at 72.218 kb on - strand, within GFF3803 at 72.379 kb on + strand, within GFF3803 at 72.435 kb on + strand, within GFF3803 at 72.558 kb on - strand, within GFF3803 at 72.788 kb on + strand, within GFF3803 at 72.789 kb on - strand, within GFF3803 at 72.790 kb on + strand, within GFF3803 at 72.790 kb on + strand, within GFF3803 at 72.790 kb on + strand, within GFF3803 at 72.790 kb on + strand, within GFF3803 at 72.902 kb on - strand, within GFF3803 at 72.986 kb on - strand at 73.043 kb on + strand at 73.098 kb on - strand at 73.730 kb on - strand, within GFF3804 at 73.826 kb on - strand, within GFF3804 at 73.826 kb on - strand, within GFF3804 at 73.905 kb on - strand, within GFF3804 at 73.918 kb on + strand, within GFF3804 at 73.945 kb on - strand, within GFF3804 at 73.947 kb on + strand, within GFF3804 at 73.948 kb on - strand, within GFF3804 at 73.956 kb on - strand, within GFF3804 at 73.957 kb on + strand, within GFF3804 at 73.992 kb on - strand, within GFF3804 at 74.009 kb on - strand, within GFF3804 at 74.028 kb on + strand, within GFF3804 at 74.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 70,959 - GFF3801 0.63 -0.3 70,981 - GFF3801 0.66 +0.5 70,999 - GFF3801 0.68 +0.7 71,080 + GFF3801 0.80 +0.2 71,184 - -0.4 71,184 - +1.0 71,272 + -0.0 71,277 - +0.7 71,538 - GFF3802 0.48 -0.2 71,539 + GFF3802 0.48 -0.6 71,970 + +0.2 72,112 + GFF3803 0.19 -0.4 72,113 - GFF3803 0.19 -1.8 72,217 + GFF3803 0.28 -0.5 72,218 - GFF3803 0.28 -0.1 72,218 - GFF3803 0.28 +0.1 72,379 + GFF3803 0.41 +0.7 72,435 + GFF3803 0.46 -0.5 72,558 - GFF3803 0.56 -0.6 72,788 + GFF3803 0.75 -0.1 72,789 - GFF3803 0.75 -0.8 72,790 + GFF3803 0.76 -0.2 72,790 + GFF3803 0.76 -0.7 72,790 + GFF3803 0.76 -0.4 72,790 + GFF3803 0.76 +0.8 72,902 - GFF3803 0.85 +0.2 72,986 - +0.3 73,043 + -1.1 73,098 - +0.3 73,730 - GFF3804 0.60 +0.2 73,826 - GFF3804 0.70 -1.1 73,826 - GFF3804 0.70 +0.1 73,905 - GFF3804 0.78 +0.3 73,918 + GFF3804 0.79 -0.1 73,945 - GFF3804 0.82 -0.1 73,947 + GFF3804 0.82 -0.1 73,948 - GFF3804 0.82 -0.7 73,956 - GFF3804 0.83 -0.4 73,957 + GFF3804 0.83 +0.1 73,992 - GFF3804 0.86 -0.8 74,009 - GFF3804 0.88 +2.0 74,028 + GFF3804 0.90 -0.0 74,029 - +0.3
Or see this region's nucleotide sequence