Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3613 and GFF3614 are separated by 216 nucleotides GFF3614 and GFF3615 are separated by 41 nucleotides GFF3615 and GFF3616 are separated by 289 nucleotides
GFF3613 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 364,015 to 365,388
GFF3613
GFF3614 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 365,605 to 366,246
GFF3614
GFF3615 - Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79), at 366,288 to 367,436
GFF3615
GFF3616 - Putative transport protein, at 367,726 to 368,931
GFF3616
Position (kb) 366
367
368 Strain fitness (log2 ratio) -1
0
1 at 365.354 kb on - strand at 365.356 kb on + strand at 365.379 kb on - strand at 365.413 kb on - strand at 365.559 kb on - strand at 366.254 kb on + strand at 366.330 kb on + strand at 366.388 kb on + strand at 366.392 kb on - strand at 366.402 kb on - strand at 366.419 kb on + strand, within GFF3615 at 366.422 kb on - strand, within GFF3615 at 366.519 kb on + strand, within GFF3615 at 366.520 kb on - strand, within GFF3615 at 366.585 kb on - strand, within GFF3615 at 366.691 kb on + strand, within GFF3615 at 366.700 kb on + strand, within GFF3615 at 366.885 kb on - strand, within GFF3615 at 366.885 kb on - strand, within GFF3615 at 366.886 kb on + strand, within GFF3615 at 366.943 kb on - strand, within GFF3615 at 366.943 kb on - strand, within GFF3615 at 367.027 kb on + strand, within GFF3615 at 367.035 kb on - strand, within GFF3615 at 367.049 kb on + strand, within GFF3615 at 367.078 kb on + strand, within GFF3615 at 367.119 kb on + strand, within GFF3615 at 367.314 kb on - strand, within GFF3615 at 367.349 kb on + strand at 367.622 kb on - strand at 367.636 kb on + strand at 367.808 kb on - strand at 367.921 kb on - strand, within GFF3616 at 367.967 kb on - strand, within GFF3616 at 368.099 kb on + strand, within GFF3616 at 368.100 kb on - strand, within GFF3616 at 368.107 kb on + strand, within GFF3616 at 368.107 kb on + strand, within GFF3616 at 368.108 kb on - strand, within GFF3616 at 368.201 kb on + strand, within GFF3616 at 368.201 kb on + strand, within GFF3616 at 368.332 kb on + strand, within GFF3616 at 368.356 kb on - strand, within GFF3616 at 368.356 kb on - strand, within GFF3616 at 368.424 kb on + strand, within GFF3616
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 365,354 - -0.7 365,356 + +0.3 365,379 - +0.3 365,413 - -0.4 365,559 - -0.5 366,254 + +1.3 366,330 + +0.4 366,388 + +0.1 366,392 - +0.4 366,402 - +0.2 366,419 + GFF3615 0.11 +0.1 366,422 - GFF3615 0.12 +0.1 366,519 + GFF3615 0.20 -0.1 366,520 - GFF3615 0.20 +0.2 366,585 - GFF3615 0.26 +0.1 366,691 + GFF3615 0.35 -0.1 366,700 + GFF3615 0.36 -1.4 366,885 - GFF3615 0.52 -1.1 366,885 - GFF3615 0.52 -0.9 366,886 + GFF3615 0.52 -0.3 366,943 - GFF3615 0.57 -0.1 366,943 - GFF3615 0.57 -0.1 367,027 + GFF3615 0.64 -0.2 367,035 - GFF3615 0.65 -0.3 367,049 + GFF3615 0.66 -0.2 367,078 + GFF3615 0.69 +0.6 367,119 + GFF3615 0.72 +0.0 367,314 - GFF3615 0.89 +0.1 367,349 + -1.1 367,622 - +0.8 367,636 + +0.4 367,808 - +0.3 367,921 - GFF3616 0.16 -0.2 367,967 - GFF3616 0.20 -0.0 368,099 + GFF3616 0.31 +0.2 368,100 - GFF3616 0.31 -0.6 368,107 + GFF3616 0.32 +1.4 368,107 + GFF3616 0.32 -0.7 368,108 - GFF3616 0.32 +0.6 368,201 + GFF3616 0.39 +0.0 368,201 + GFF3616 0.39 +0.2 368,332 + GFF3616 0.50 +0.6 368,356 - GFF3616 0.52 -0.1 368,356 - GFF3616 0.52 +0.1 368,424 + GFF3616 0.58 -0.1
Or see this region's nucleotide sequence