Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF349 and GFF350 are separated by 102 nucleotides GFF350 and GFF351 are separated by 150 nucleotides
GFF349 - Alpha-xylosidase (EC 3.2.1.-), at 66,184 to 68,502
GFF349
GFF350 - FIG01200701: possible membrane protein, at 68,605 to 70,284
GFF350
GFF351 - Xanthine permease, at 70,435 to 71,826
GFF351
Position (kb)
68
69
70
71 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 67.633 kb on + strand, within GFF349 at 67.835 kb on - strand, within GFF349 at 67.897 kb on + strand, within GFF349 at 68.040 kb on - strand, within GFF349 at 68.181 kb on + strand, within GFF349 at 68.182 kb on - strand, within GFF349 at 68.182 kb on - strand, within GFF349 at 68.187 kb on - strand, within GFF349 at 68.403 kb on + strand at 68.403 kb on + strand at 68.459 kb on + strand at 68.460 kb on - strand at 68.460 kb on - strand at 68.494 kb on - strand at 68.500 kb on + strand at 68.546 kb on + strand at 68.629 kb on - strand at 68.671 kb on + strand at 68.702 kb on - strand at 68.745 kb on + strand at 68.770 kb on - strand at 68.815 kb on + strand, within GFF350 at 68.830 kb on + strand, within GFF350 at 69.206 kb on - strand, within GFF350 at 69.231 kb on + strand, within GFF350 at 69.232 kb on - strand, within GFF350 at 69.233 kb on + strand, within GFF350 at 69.556 kb on - strand, within GFF350 at 69.579 kb on + strand, within GFF350 at 69.661 kb on - strand, within GFF350 at 69.750 kb on + strand, within GFF350 at 69.987 kb on + strand, within GFF350 at 70.062 kb on + strand, within GFF350 at 70.063 kb on - strand, within GFF350 at 70.067 kb on - strand, within GFF350 at 70.127 kb on - strand at 70.127 kb on - strand at 70.199 kb on - strand at 70.247 kb on + strand at 70.268 kb on - strand at 70.287 kb on - strand at 70.349 kb on + strand at 70.349 kb on + strand at 70.350 kb on - strand at 70.398 kb on + strand at 70.635 kb on + strand, within GFF351 at 70.778 kb on + strand, within GFF351 at 70.846 kb on - strand, within GFF351 at 70.849 kb on - strand, within GFF351 at 70.914 kb on - strand, within GFF351 at 70.987 kb on - strand, within GFF351 at 71.045 kb on + strand, within GFF351 at 71.045 kb on + strand, within GFF351 at 71.046 kb on - strand, within GFF351 at 71.179 kb on - strand, within GFF351
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 67,633 + GFF349 0.62 -1.2 67,835 - GFF349 0.71 +0.2 67,897 + GFF349 0.74 +0.5 68,040 - GFF349 0.80 +0.2 68,181 + GFF349 0.86 -0.7 68,182 - GFF349 0.86 +0.2 68,182 - GFF349 0.86 -0.1 68,187 - GFF349 0.86 -0.5 68,403 + -0.6 68,403 + +0.7 68,459 + +0.6 68,460 - -1.3 68,460 - +2.1 68,494 - +0.4 68,500 + -0.4 68,546 + -0.1 68,629 - +0.3 68,671 + -0.3 68,702 - +1.1 68,745 + +1.0 68,770 - +0.5 68,815 + GFF350 0.12 -0.1 68,830 + GFF350 0.13 -1.1 69,206 - GFF350 0.36 -0.9 69,231 + GFF350 0.37 -0.4 69,232 - GFF350 0.37 -0.5 69,233 + GFF350 0.37 -0.8 69,556 - GFF350 0.57 -0.5 69,579 + GFF350 0.58 +0.5 69,661 - GFF350 0.63 +0.5 69,750 + GFF350 0.68 +0.1 69,987 + GFF350 0.82 -0.6 70,062 + GFF350 0.87 +1.0 70,063 - GFF350 0.87 +0.0 70,067 - GFF350 0.87 -0.0 70,127 - -0.1 70,127 - -2.3 70,199 - +1.3 70,247 + -0.2 70,268 - -0.2 70,287 - -0.1 70,349 + -0.1 70,349 + +0.2 70,350 - -0.3 70,398 + +0.1 70,635 + GFF351 0.14 +0.1 70,778 + GFF351 0.25 +0.2 70,846 - GFF351 0.30 -0.2 70,849 - GFF351 0.30 +0.1 70,914 - GFF351 0.34 +0.6 70,987 - GFF351 0.40 -0.4 71,045 + GFF351 0.44 +0.3 71,045 + GFF351 0.44 -0.2 71,046 - GFF351 0.44 -0.2 71,179 - GFF351 0.53 +0.2
Or see this region's nucleotide sequence