Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3097 and GFF3098 are separated by 226 nucleotides GFF3098 and GFF3099 are separated by 67 nucleotides GFF3099 and GFF3100 overlap by 1 nucleotides
GFF3097 - Glutathionylspermidine synthase (EC 6.3.1.8) / Glutathionylspermidine amidohydrolase (EC 3.5.1.78), at 107,482 to 109,338
GFF3097
GFF3098 - Uncharacterized GST-like protein yghU associated with glutathionylspermidine synthetase/amidase, at 109,565 to 110,431
GFF3098
GFF3099 - Molybdenum-binding periplasmic protein, at 110,499 to 111,209
GFF3099
GFF3100 - Possible ABC-transport protein, periplasmic-binding component, at 111,209 to 112,261
GFF3100
Position (kb)
109
110
111 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 108.587 kb on + strand, within GFF3097 at 108.628 kb on + strand, within GFF3097 at 108.629 kb on - strand, within GFF3097 at 108.633 kb on - strand, within GFF3097 at 108.753 kb on - strand, within GFF3097 at 108.753 kb on - strand, within GFF3097 at 108.822 kb on - strand, within GFF3097 at 109.177 kb on - strand at 109.187 kb on + strand at 109.187 kb on + strand at 109.188 kb on - strand at 109.262 kb on + strand at 109.345 kb on - strand at 109.430 kb on + strand at 109.431 kb on - strand at 109.431 kb on - strand at 109.527 kb on - strand at 109.639 kb on + strand at 109.698 kb on + strand, within GFF3098 at 109.741 kb on + strand, within GFF3098 at 109.742 kb on - strand, within GFF3098 at 110.057 kb on + strand, within GFF3098 at 110.092 kb on + strand, within GFF3098 at 110.145 kb on + strand, within GFF3098 at 110.177 kb on + strand, within GFF3098 at 110.182 kb on + strand, within GFF3098 at 110.183 kb on - strand, within GFF3098 at 110.183 kb on - strand, within GFF3098 at 110.237 kb on + strand, within GFF3098 at 110.237 kb on + strand, within GFF3098 at 110.271 kb on - strand, within GFF3098 at 110.280 kb on + strand, within GFF3098 at 110.281 kb on - strand, within GFF3098 at 110.451 kb on + strand at 110.454 kb on + strand at 110.454 kb on + strand at 110.475 kb on + strand at 110.702 kb on - strand, within GFF3099 at 110.929 kb on - strand, within GFF3099 at 111.109 kb on - strand, within GFF3099 at 111.136 kb on - strand, within GFF3099 at 111.211 kb on - strand at 111.216 kb on - strand at 111.216 kb on - strand at 111.225 kb on + strand at 111.233 kb on - strand at 111.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 108,587 + GFF3097 0.60 -0.2 108,628 + GFF3097 0.62 -0.6 108,629 - GFF3097 0.62 +0.2 108,633 - GFF3097 0.62 +0.6 108,753 - GFF3097 0.68 -0.2 108,753 - GFF3097 0.68 -0.9 108,822 - GFF3097 0.72 +0.1 109,177 - +0.8 109,187 + +0.5 109,187 + -0.6 109,188 - -1.4 109,262 + -0.6 109,345 - -0.0 109,430 + -0.2 109,431 - -0.2 109,431 - -0.0 109,527 - +0.2 109,639 + +0.2 109,698 + GFF3098 0.15 +0.3 109,741 + GFF3098 0.20 +1.1 109,742 - GFF3098 0.20 -0.4 110,057 + GFF3098 0.57 +1.1 110,092 + GFF3098 0.61 +0.2 110,145 + GFF3098 0.67 +0.1 110,177 + GFF3098 0.71 -0.1 110,182 + GFF3098 0.71 -0.0 110,183 - GFF3098 0.71 -0.0 110,183 - GFF3098 0.71 +0.3 110,237 + GFF3098 0.78 +0.0 110,237 + GFF3098 0.78 -1.0 110,271 - GFF3098 0.81 +1.8 110,280 + GFF3098 0.82 +1.8 110,281 - GFF3098 0.83 +0.3 110,451 + -1.8 110,454 + +0.5 110,454 + +0.4 110,475 + +0.6 110,702 - GFF3099 0.29 -0.0 110,929 - GFF3099 0.60 +0.1 111,109 - GFF3099 0.86 +1.7 111,136 - GFF3099 0.90 -0.1 111,211 - +1.0 111,216 - -0.8 111,216 - -0.8 111,225 + -0.1 111,233 - -0.2 111,249 + +0.0
Or see this region's nucleotide sequence