Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3088 and GFF3089 are separated by 307 nucleotides GFF3089 and GFF3090 are separated by 262 nucleotides GFF3090 and GFF3091 are separated by 38 nucleotides
GFF3088 - Putative cytoplasmic protein USSDB7A, at 96,107 to 96,592
GFF3088
GFF3089 - FIG01045853: hypothetical protein, at 96,900 to 97,055
GFF3089
GFF3090 - Putative xylanase, at 97,318 to 98,241
GFF3090
GFF3091 - Possible hydrolase, at 98,280 to 99,158
GFF3091
Position (kb) 97
98
99 Strain fitness (log2 ratio) -1
0
1 at 96.359 kb on + strand, within GFF3088 at 96.378 kb on + strand, within GFF3088 at 96.379 kb on - strand, within GFF3088 at 96.379 kb on - strand, within GFF3088 at 96.522 kb on - strand, within GFF3088 at 96.562 kb on + strand at 96.669 kb on - strand at 96.777 kb on - strand at 97.467 kb on + strand, within GFF3090 at 97.468 kb on - strand, within GFF3090 at 97.484 kb on - strand, within GFF3090 at 97.484 kb on - strand, within GFF3090 at 97.497 kb on + strand, within GFF3090 at 97.497 kb on + strand, within GFF3090 at 97.544 kb on - strand, within GFF3090 at 97.566 kb on - strand, within GFF3090 at 97.572 kb on - strand, within GFF3090 at 97.641 kb on - strand, within GFF3090 at 97.667 kb on + strand, within GFF3090 at 97.754 kb on + strand, within GFF3090 at 97.790 kb on + strand, within GFF3090 at 97.833 kb on + strand, within GFF3090 at 97.834 kb on - strand, within GFF3090 at 97.834 kb on - strand, within GFF3090 at 97.887 kb on + strand, within GFF3090 at 97.888 kb on - strand, within GFF3090 at 98.439 kb on - strand, within GFF3091 at 98.613 kb on - strand, within GFF3091 at 98.703 kb on - strand, within GFF3091 at 98.744 kb on + strand, within GFF3091 at 98.761 kb on + strand, within GFF3091 at 98.786 kb on + strand, within GFF3091 at 98.795 kb on + strand, within GFF3091 at 98.807 kb on - strand, within GFF3091 at 98.831 kb on + strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.934 kb on - strand, within GFF3091
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 96,359 + GFF3088 0.52 -1.3 96,378 + GFF3088 0.56 +0.7 96,379 - GFF3088 0.56 -0.3 96,379 - GFF3088 0.56 +0.8 96,522 - GFF3088 0.85 -0.1 96,562 + +0.1 96,669 - +0.3 96,777 - +0.5 97,467 + GFF3090 0.16 -0.1 97,468 - GFF3090 0.16 -0.2 97,484 - GFF3090 0.18 -0.2 97,484 - GFF3090 0.18 -0.3 97,497 + GFF3090 0.19 -0.1 97,497 + GFF3090 0.19 -0.8 97,544 - GFF3090 0.24 -0.9 97,566 - GFF3090 0.27 +0.1 97,572 - GFF3090 0.27 -0.3 97,641 - GFF3090 0.35 -0.5 97,667 + GFF3090 0.38 +0.6 97,754 + GFF3090 0.47 -0.2 97,790 + GFF3090 0.51 +0.7 97,833 + GFF3090 0.56 -0.0 97,834 - GFF3090 0.56 -0.0 97,834 - GFF3090 0.56 -0.5 97,887 + GFF3090 0.62 -0.5 97,888 - GFF3090 0.62 -0.4 98,439 - GFF3091 0.18 +0.3 98,613 - GFF3091 0.38 +0.7 98,703 - GFF3091 0.48 -0.0 98,744 + GFF3091 0.53 -0.5 98,761 + GFF3091 0.55 -0.3 98,786 + GFF3091 0.58 -0.4 98,795 + GFF3091 0.59 +0.8 98,807 - GFF3091 0.60 -0.1 98,831 + GFF3091 0.63 -0.7 98,832 - GFF3091 0.63 -0.3 98,832 - GFF3091 0.63 +1.3 98,832 - GFF3091 0.63 +0.2 98,832 - GFF3091 0.63 -0.2 98,934 - GFF3091 0.74 -0.9
Or see this region's nucleotide sequence