Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2780 and GFF2781 are separated by 124 nucleotides GFF2781 and GFF2782 are separated by 44 nucleotides GFF2782 and GFF2783 are separated by 275 nucleotides
GFF2780 - Ribose-phosphate pyrophosphokinase (EC 2.7.6.1), at 303,030 to 303,977
GFF2780
GFF2781 - Putative sulfate permease, at 304,102 to 305,787
GFF2781
GFF2782 - FIG00554739: membrane protein YchH, at 305,832 to 306,110
GFF2782
GFF2783 - Peptidyl-tRNA hydrolase (EC 3.1.1.29), at 306,386 to 306,970
GFF2783
Position (kb) 304
305
306 Strain fitness (log2 ratio) -1
0
1 at 304.042 kb on + strand at 304.043 kb on - strand at 304.065 kb on - strand at 304.065 kb on - strand at 304.279 kb on + strand, within GFF2781 at 304.290 kb on + strand, within GFF2781 at 304.291 kb on - strand, within GFF2781 at 304.291 kb on - strand, within GFF2781 at 304.342 kb on - strand, within GFF2781 at 304.357 kb on - strand, within GFF2781 at 304.489 kb on + strand, within GFF2781 at 304.490 kb on - strand, within GFF2781 at 304.533 kb on + strand, within GFF2781 at 304.536 kb on + strand, within GFF2781 at 304.600 kb on + strand, within GFF2781 at 305.025 kb on + strand, within GFF2781 at 305.026 kb on - strand, within GFF2781 at 305.026 kb on - strand, within GFF2781 at 305.031 kb on + strand, within GFF2781 at 305.210 kb on + strand, within GFF2781 at 305.269 kb on - strand, within GFF2781 at 305.284 kb on - strand, within GFF2781 at 305.331 kb on + strand, within GFF2781 at 305.367 kb on + strand, within GFF2781 at 305.367 kb on + strand, within GFF2781 at 305.416 kb on - strand, within GFF2781 at 305.440 kb on - strand, within GFF2781 at 305.550 kb on + strand, within GFF2781 at 305.550 kb on + strand, within GFF2781 at 305.566 kb on - strand, within GFF2781 at 305.837 kb on + strand at 305.876 kb on - strand, within GFF2782 at 306.111 kb on - strand at 306.345 kb on + strand at 306.346 kb on - strand at 306.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 304,042 + -0.2 304,043 - +0.4 304,065 - +1.2 304,065 - +0.5 304,279 + GFF2781 0.10 +0.3 304,290 + GFF2781 0.11 -0.9 304,291 - GFF2781 0.11 +1.5 304,291 - GFF2781 0.11 +0.0 304,342 - GFF2781 0.14 +0.7 304,357 - GFF2781 0.15 -0.1 304,489 + GFF2781 0.23 +0.3 304,490 - GFF2781 0.23 -0.8 304,533 + GFF2781 0.26 +1.2 304,536 + GFF2781 0.26 -0.1 304,600 + GFF2781 0.30 +1.4 305,025 + GFF2781 0.55 +0.5 305,026 - GFF2781 0.55 +1.1 305,026 - GFF2781 0.55 +0.4 305,031 + GFF2781 0.55 -0.0 305,210 + GFF2781 0.66 -0.1 305,269 - GFF2781 0.69 -0.1 305,284 - GFF2781 0.70 +0.8 305,331 + GFF2781 0.73 +1.3 305,367 + GFF2781 0.75 +0.5 305,367 + GFF2781 0.75 +0.2 305,416 - GFF2781 0.78 +0.5 305,440 - GFF2781 0.79 -0.1 305,550 + GFF2781 0.86 +0.2 305,550 + GFF2781 0.86 +0.3 305,566 - GFF2781 0.87 +0.1 305,837 + -0.0 305,876 - GFF2782 0.16 +0.3 306,111 - +0.2 306,345 + +0.9 306,346 - -0.4 306,350 + -0.5
Or see this region's nucleotide sequence