Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2748 and GFF2749 are separated by 476 nucleotides GFF2749 and GFF2750 are separated by 196 nucleotides
GFF2748 - Putative membrane protein, at 267,622 to 268,269
GFF2748
GFF2749 - Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10), at 268,746 to 271,424
GFF2749
GFF2750 - Thymidine kinase (EC 2.7.1.21), at 271,621 to 272,238
GFF2750
Position (kb) 268
269
270
271
272 Strain fitness (log2 ratio) -2
-1
0
1
2 at 268.050 kb on + strand, within GFF2748 at 268.352 kb on - strand at 268.363 kb on + strand at 268.364 kb on - strand at 268.368 kb on - strand at 268.401 kb on - strand at 268.406 kb on + strand at 268.463 kb on - strand at 268.514 kb on + strand at 268.583 kb on - strand at 268.970 kb on + strand at 268.974 kb on + strand at 268.985 kb on + strand at 268.985 kb on + strand at 269.269 kb on + strand, within GFF2749 at 269.352 kb on + strand, within GFF2749 at 269.775 kb on + strand, within GFF2749 at 269.776 kb on - strand, within GFF2749 at 269.776 kb on - strand, within GFF2749 at 269.895 kb on + strand, within GFF2749 at 269.896 kb on - strand, within GFF2749 at 270.080 kb on + strand, within GFF2749 at 270.080 kb on + strand, within GFF2749 at 270.170 kb on + strand, within GFF2749 at 270.420 kb on + strand, within GFF2749 at 270.420 kb on + strand, within GFF2749 at 270.421 kb on - strand, within GFF2749 at 270.483 kb on - strand, within GFF2749 at 270.487 kb on - strand, within GFF2749 at 270.619 kb on - strand, within GFF2749 at 270.907 kb on + strand, within GFF2749 at 270.908 kb on - strand, within GFF2749 at 270.983 kb on + strand, within GFF2749 at 270.997 kb on - strand, within GFF2749 at 271.042 kb on - strand, within GFF2749 at 271.329 kb on + strand at 271.510 kb on + strand at 271.567 kb on - strand at 271.620 kb on - strand at 271.620 kb on - strand at 271.624 kb on + strand at 271.625 kb on - strand at 271.625 kb on - strand at 271.681 kb on - strand at 271.783 kb on - strand, within GFF2750 at 271.915 kb on - strand, within GFF2750 at 271.918 kb on + strand, within GFF2750 at 271.919 kb on - strand, within GFF2750 at 271.996 kb on + strand, within GFF2750 at 271.996 kb on + strand, within GFF2750 at 272.044 kb on - strand, within GFF2750 at 272.131 kb on - strand, within GFF2750 at 272.135 kb on - strand, within GFF2750 at 272.135 kb on - strand, within GFF2750 at 272.225 kb on + strand at 272.226 kb on - strand at 272.289 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 268,050 + GFF2748 0.66 +0.3 268,352 - +0.4 268,363 + +0.5 268,364 - +0.4 268,368 - +0.3 268,401 - -0.0 268,406 + +0.5 268,463 - +0.0 268,514 + -0.5 268,583 - +0.0 268,970 + +0.3 268,974 + +0.7 268,985 + +0.8 268,985 + -0.3 269,269 + GFF2749 0.20 +0.8 269,352 + GFF2749 0.23 -0.4 269,775 + GFF2749 0.38 +0.4 269,776 - GFF2749 0.38 -0.3 269,776 - GFF2749 0.38 -0.4 269,895 + GFF2749 0.43 -0.7 269,896 - GFF2749 0.43 -0.1 270,080 + GFF2749 0.50 -0.5 270,080 + GFF2749 0.50 +0.2 270,170 + GFF2749 0.53 -0.5 270,420 + GFF2749 0.62 +0.5 270,420 + GFF2749 0.62 +0.5 270,421 - GFF2749 0.63 +1.3 270,483 - GFF2749 0.65 +0.6 270,487 - GFF2749 0.65 +0.3 270,619 - GFF2749 0.70 -0.2 270,907 + GFF2749 0.81 +0.1 270,908 - GFF2749 0.81 +1.1 270,983 + GFF2749 0.84 -0.4 270,997 - GFF2749 0.84 +0.4 271,042 - GFF2749 0.86 +0.1 271,329 + +1.9 271,510 + +0.9 271,567 - -2.7 271,620 - -0.7 271,620 - +0.1 271,624 + +0.3 271,625 - +0.5 271,625 - +0.6 271,681 - +0.6 271,783 - GFF2750 0.26 -0.6 271,915 - GFF2750 0.48 -0.1 271,918 + GFF2750 0.48 +1.6 271,919 - GFF2750 0.48 -0.7 271,996 + GFF2750 0.61 +1.7 271,996 + GFF2750 0.61 +0.9 272,044 - GFF2750 0.68 -0.7 272,131 - GFF2750 0.83 +1.1 272,135 - GFF2750 0.83 +0.5 272,135 - GFF2750 0.83 +1.2 272,225 + +0.7 272,226 - -0.0 272,289 + +0.9
Or see this region's nucleotide sequence