Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2719 and GFF2720 are separated by 100 nucleotides GFF2720 and GFF2721 are separated by 7 nucleotides GFF2721 and GFF2722 are separated by 293 nucleotides
GFF2719 - Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70), at 241,422 to 242,297
GFF2719
GFF2720 - Hypothetical YciO protein, TsaC/YrdC paralog, at 242,398 to 243,018
GFF2720
GFF2721 - COG0613, Predicted metal-dependent phosphoesterases (PHP family), at 243,026 to 243,874
GFF2721
GFF2722 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 244,168 to 245,730
GFF2722
Position (kb) 243
244 Strain fitness (log2 ratio) -1
0
1
2
3 at 242.081 kb on - strand, within GFF2719 at 242.297 kb on + strand at 242.399 kb on + strand at 242.402 kb on - strand at 242.671 kb on - strand, within GFF2720 at 242.730 kb on - strand, within GFF2720 at 242.781 kb on - strand, within GFF2720 at 242.787 kb on - strand, within GFF2720 at 242.932 kb on - strand, within GFF2720 at 242.932 kb on - strand, within GFF2720 at 243.001 kb on - strand at 243.003 kb on - strand at 243.361 kb on + strand, within GFF2721 at 243.631 kb on + strand, within GFF2721 at 243.809 kb on + strand at 243.810 kb on - strand at 243.914 kb on - strand at 243.914 kb on - strand at 243.914 kb on - strand at 243.928 kb on + strand at 243.929 kb on - strand at 243.929 kb on - strand at 243.929 kb on - strand at 243.936 kb on - strand at 243.951 kb on + strand at 243.951 kb on + strand at 243.953 kb on + strand at 243.962 kb on - strand at 244.025 kb on + strand at 244.087 kb on - strand at 244.335 kb on + strand, within GFF2722 at 244.475 kb on + strand, within GFF2722 at 244.596 kb on + strand, within GFF2722 at 244.596 kb on + strand, within GFF2722 at 244.685 kb on - strand, within GFF2722 at 244.812 kb on + strand, within GFF2722 at 244.823 kb on + strand, within GFF2722
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 242,081 - GFF2719 0.75 -1.3 242,297 + +0.2 242,399 + +0.5 242,402 - +1.1 242,671 - GFF2720 0.44 -0.2 242,730 - GFF2720 0.53 +1.0 242,781 - GFF2720 0.62 -0.1 242,787 - GFF2720 0.63 +1.1 242,932 - GFF2720 0.86 +0.2 242,932 - GFF2720 0.86 +0.6 243,001 - +0.5 243,003 - +2.2 243,361 + GFF2721 0.39 +0.2 243,631 + GFF2721 0.71 +2.2 243,809 + -0.4 243,810 - -0.3 243,914 - +0.1 243,914 - -0.2 243,914 - +0.3 243,928 + +0.1 243,929 - +1.0 243,929 - +0.0 243,929 - -0.0 243,936 - +0.5 243,951 + +0.5 243,951 + -0.4 243,953 + +0.0 243,962 - +0.6 244,025 + +0.3 244,087 - +0.3 244,335 + GFF2722 0.11 -0.2 244,475 + GFF2722 0.20 +0.1 244,596 + GFF2722 0.27 +0.4 244,596 + GFF2722 0.27 +0.0 244,685 - GFF2722 0.33 +3.3 244,812 + GFF2722 0.41 +0.8 244,823 + GFF2722 0.42 +0.2
Or see this region's nucleotide sequence