Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2651 and GFF2652 are separated by 52 nucleotides GFF2652 and GFF2653 are separated by 78 nucleotides GFF2653 and GFF2654 overlap by 20 nucleotides GFF2654 and GFF2655 are separated by 257 nucleotides GFF2655 and GFF2656 are separated by 195 nucleotides
GFF2651 - Putative methyl-accepting chemotaxis protein, at 176,895 to 178,007
GFF2651
GFF2652 - FIG01046553: hypothetical protein, at 178,060 to 178,221
GFF2652
GFF2653 - FIG01045924: hypothetical protein, at 178,300 to 178,479
GFF2653
GFF2654 - FIG00638524: hypothetical protein, at 178,460 to 179,023
GFF2654
GFF2655 - Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 179,281 to 179,796
GFF2655
GFF2656 - Fumarate and nitrate reduction regulatory protein, at 179,992 to 180,744
GFF2656
Position (kb) 178
179
180 Strain fitness (log2 ratio) -1
0
1
2 at 177.471 kb on - strand, within GFF2651 at 177.546 kb on + strand, within GFF2651 at 177.603 kb on + strand, within GFF2651 at 177.652 kb on - strand, within GFF2651 at 177.762 kb on - strand, within GFF2651 at 177.809 kb on + strand, within GFF2651 at 177.846 kb on - strand, within GFF2651 at 177.890 kb on + strand, within GFF2651 at 177.892 kb on + strand, within GFF2651 at 177.980 kb on + strand at 178.002 kb on + strand at 178.245 kb on - strand at 178.395 kb on + strand, within GFF2653 at 178.396 kb on - strand, within GFF2653 at 178.468 kb on + strand at 178.516 kb on + strand at 178.531 kb on - strand, within GFF2654 at 178.580 kb on + strand, within GFF2654 at 178.614 kb on + strand, within GFF2654 at 178.614 kb on + strand, within GFF2654 at 178.678 kb on + strand, within GFF2654 at 178.861 kb on + strand, within GFF2654 at 178.888 kb on + strand, within GFF2654 at 178.949 kb on + strand, within GFF2654 at 179.163 kb on - strand at 179.170 kb on + strand at 179.239 kb on - strand at 179.239 kb on - strand at 179.250 kb on + strand at 179.255 kb on + strand at 179.392 kb on - strand, within GFF2655 at 179.991 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 177,471 - GFF2651 0.52 -0.2 177,546 + GFF2651 0.58 +0.4 177,603 + GFF2651 0.64 +1.4 177,652 - GFF2651 0.68 +0.2 177,762 - GFF2651 0.78 -0.4 177,809 + GFF2651 0.82 +0.4 177,846 - GFF2651 0.85 +0.7 177,890 + GFF2651 0.89 -0.4 177,892 + GFF2651 0.90 -0.1 177,980 + +0.4 178,002 + -0.7 178,245 - +1.8 178,395 + GFF2653 0.53 +0.1 178,396 - GFF2653 0.53 +0.1 178,468 + +0.5 178,516 + +0.1 178,531 - GFF2654 0.13 +0.9 178,580 + GFF2654 0.21 -1.0 178,614 + GFF2654 0.27 +0.7 178,614 + GFF2654 0.27 -0.2 178,678 + GFF2654 0.39 +0.2 178,861 + GFF2654 0.71 -0.1 178,888 + GFF2654 0.76 +0.2 178,949 + GFF2654 0.87 +2.6 179,163 - -0.1 179,170 + +0.2 179,239 - +0.3 179,239 - -1.3 179,250 + -0.0 179,255 + -1.3 179,392 - GFF2655 0.22 -0.5 179,991 + -0.1
Or see this region's nucleotide sequence