Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2382 and GFF2383 are separated by 45 nucleotides GFF2383 and GFF2384 are separated by 25 nucleotides GFF2384 and GFF2385 are separated by 40 nucleotides GFF2385 and GFF2386 are separated by 107 nucleotides GFF2386 and GFF2387 are separated by 10 nucleotides
GFF2382 - SSU ribosomal protein S11p (S14e), at 13,005 to 13,394
GFF2382
GFF2383 - SSU ribosomal protein S4p (S9e), at 13,440 to 14,048
GFF2383
GFF2384 - DNA-directed RNA polymerase alpha subunit (EC 2.7.7.6), at 14,074 to 15,063
GFF2384
GFF2385 - LSU ribosomal protein L17p, at 15,104 to 15,487
GFF2385
GFF2386 - FIG004614: Putative cytoplasmic protein, at 15,595 to 15,963
GFF2386
GFF2387 - transcriptional regulator, MerR family, at 15,974 to 16,399
GFF2387
Position (kb) 14
15
16 Strain fitness (log2 ratio) -1
0
1 at 15.549 kb on + strand at 15.549 kb on + strand at 15.549 kb on + strand at 15.602 kb on + strand at 15.793 kb on - strand, within GFF2386 at 15.793 kb on - strand, within GFF2386 at 15.800 kb on + strand, within GFF2386 at 15.925 kb on + strand, within GFF2386 at 15.961 kb on + strand at 15.974 kb on - strand at 15.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 15,549 + -0.9 15,549 + -0.3 15,549 + -0.0 15,602 + -0.8 15,793 - GFF2386 0.54 -0.0 15,793 - GFF2386 0.54 +0.3 15,800 + GFF2386 0.56 -0.1 15,925 + GFF2386 0.89 -0.7 15,961 + -1.3 15,974 - +0.2 15,974 - +0.5
Or see this region's nucleotide sequence