Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1534 and GFF1535 are separated by 529 nucleotides GFF1535 and GFF1536 are separated by 25 nucleotides
GFF1534 - D-galactarate dehydratase (EC 4.2.1.42), at 38,318 to 39,889
GFF1534
GFF1535 - 2-dehydro-3-deoxyglucarate aldolase (EC 4.1.2.20), at 40,419 to 41,189
GFF1535
GFF1536 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 41,215 to 42,105
GFF1536
Position (kb) 40
41
42 Strain fitness (log2 ratio) -1
0
1
2 at 39.438 kb on + strand, within GFF1534 at 39.439 kb on - strand, within GFF1534 at 39.439 kb on - strand, within GFF1534 at 39.439 kb on - strand, within GFF1534 at 39.476 kb on - strand, within GFF1534 at 39.522 kb on + strand, within GFF1534 at 39.536 kb on - strand, within GFF1534 at 39.589 kb on + strand, within GFF1534 at 39.590 kb on - strand, within GFF1534 at 40.102 kb on - strand at 40.245 kb on + strand at 40.360 kb on + strand at 40.430 kb on + strand at 40.592 kb on - strand, within GFF1535 at 40.809 kb on - strand, within GFF1535 at 40.862 kb on + strand, within GFF1535 at 40.863 kb on - strand, within GFF1535 at 40.863 kb on - strand, within GFF1535 at 41.022 kb on - strand, within GFF1535 at 41.137 kb on + strand at 41.243 kb on + strand at 41.244 kb on - strand at 41.244 kb on - strand at 41.416 kb on - strand, within GFF1536 at 41.416 kb on - strand, within GFF1536 at 41.458 kb on - strand, within GFF1536 at 41.496 kb on + strand, within GFF1536 at 41.497 kb on - strand, within GFF1536 at 41.656 kb on + strand, within GFF1536 at 41.657 kb on - strand, within GFF1536 at 41.660 kb on - strand, within GFF1536 at 41.931 kb on + strand, within GFF1536 at 41.932 kb on - strand, within GFF1536 at 41.956 kb on - strand, within GFF1536 at 42.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 39,438 + GFF1534 0.71 -1.1 39,439 - GFF1534 0.71 +0.6 39,439 - GFF1534 0.71 +1.9 39,439 - GFF1534 0.71 -0.2 39,476 - GFF1534 0.74 -0.1 39,522 + GFF1534 0.77 -0.1 39,536 - GFF1534 0.77 +0.6 39,589 + GFF1534 0.81 +0.4 39,590 - GFF1534 0.81 -0.1 40,102 - +0.2 40,245 + -0.0 40,360 + +0.8 40,430 + +0.9 40,592 - GFF1535 0.22 +0.0 40,809 - GFF1535 0.51 +2.2 40,862 + GFF1535 0.57 +0.4 40,863 - GFF1535 0.58 -0.3 40,863 - GFF1535 0.58 -0.2 41,022 - GFF1535 0.78 +1.2 41,137 + +0.7 41,243 + -0.5 41,244 - -0.6 41,244 - +0.1 41,416 - GFF1536 0.23 -0.6 41,416 - GFF1536 0.23 +0.3 41,458 - GFF1536 0.27 +0.4 41,496 + GFF1536 0.32 -0.9 41,497 - GFF1536 0.32 -0.1 41,656 + GFF1536 0.49 +0.4 41,657 - GFF1536 0.50 +0.7 41,660 - GFF1536 0.50 +0.6 41,931 + GFF1536 0.80 -0.2 41,932 - GFF1536 0.80 +2.3 41,956 - GFF1536 0.83 -0.6 42,067 + +0.2
Or see this region's nucleotide sequence