Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1482 and GFF1483 are separated by 13 nucleotides GFF1483 and GFF1484 overlap by 8 nucleotides GFF1484 and GFF1485 are separated by 75 nucleotides
GFF1482 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 152,011 to 154,440
GFF1482
GFF1483 - Anaerobic dimethyl sulfoxide reductase chain B (EC 1.8.5.3), at 154,454 to 155,080
GFF1483
GFF1484 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 155,073 to 155,846
GFF1484
GFF1485 - Putative oxidoreductase component of anaerobic dehydrogenases; Chaperone protein TorD, at 155,922 to 156,515
GFF1485
Position (kb)
154
155
156 Strain fitness (log2 ratio)
-1
0
1
2
3 at 153.550 kb on + strand, within GFF1482 at 153.551 kb on + strand, within GFF1482 at 153.581 kb on - strand, within GFF1482 at 153.635 kb on - strand, within GFF1482 at 153.689 kb on - strand, within GFF1482 at 153.736 kb on + strand, within GFF1482 at 153.736 kb on + strand, within GFF1482 at 153.738 kb on + strand, within GFF1482 at 153.913 kb on - strand, within GFF1482 at 153.913 kb on - strand, within GFF1482 at 154.111 kb on - strand, within GFF1482 at 154.124 kb on - strand, within GFF1482 at 154.226 kb on + strand at 154.451 kb on - strand at 154.472 kb on + strand at 154.473 kb on - strand at 154.574 kb on + strand, within GFF1483 at 154.575 kb on - strand, within GFF1483 at 154.627 kb on + strand, within GFF1483 at 154.627 kb on + strand, within GFF1483 at 154.628 kb on - strand, within GFF1483 at 154.631 kb on + strand, within GFF1483 at 154.632 kb on - strand, within GFF1483 at 154.635 kb on - strand, within GFF1483 at 154.651 kb on + strand, within GFF1483 at 154.746 kb on + strand, within GFF1483 at 154.938 kb on - strand, within GFF1483 at 155.000 kb on - strand, within GFF1483 at 155.002 kb on + strand, within GFF1483 at 155.108 kb on + strand at 155.437 kb on + strand, within GFF1484 at 155.493 kb on + strand, within GFF1484 at 155.494 kb on - strand, within GFF1484 at 155.512 kb on + strand, within GFF1484 at 155.625 kb on + strand, within GFF1484 at 155.626 kb on - strand, within GFF1484 at 155.628 kb on - strand, within GFF1484 at 155.675 kb on + strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.861 kb on + strand at 155.870 kb on + strand at 155.870 kb on + strand at 155.870 kb on + strand at 155.871 kb on - strand at 155.915 kb on + strand at 155.921 kb on + strand at 155.968 kb on - strand at 156.057 kb on - strand, within GFF1485
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 153,550 + GFF1482 0.63 +0.5 153,551 + GFF1482 0.63 -0.7 153,581 - GFF1482 0.65 +0.3 153,635 - GFF1482 0.67 -0.1 153,689 - GFF1482 0.69 -0.2 153,736 + GFF1482 0.71 -0.6 153,736 + GFF1482 0.71 -0.4 153,738 + GFF1482 0.71 -0.1 153,913 - GFF1482 0.78 -0.6 153,913 - GFF1482 0.78 +0.1 154,111 - GFF1482 0.86 +0.6 154,124 - GFF1482 0.87 +0.5 154,226 + +0.6 154,451 - -0.3 154,472 + +1.4 154,473 - +0.4 154,574 + GFF1483 0.19 +0.6 154,575 - GFF1483 0.19 -0.1 154,627 + GFF1483 0.28 +0.1 154,627 + GFF1483 0.28 +0.2 154,628 - GFF1483 0.28 +0.2 154,631 + GFF1483 0.28 -0.0 154,632 - GFF1483 0.28 +0.7 154,635 - GFF1483 0.29 +2.8 154,651 + GFF1483 0.31 -0.5 154,746 + GFF1483 0.47 +0.1 154,938 - GFF1483 0.77 +1.0 155,000 - GFF1483 0.87 +1.0 155,002 + GFF1483 0.87 +0.3 155,108 + -0.2 155,437 + GFF1484 0.47 +0.9 155,493 + GFF1484 0.54 -0.2 155,494 - GFF1484 0.54 -0.2 155,512 + GFF1484 0.57 +0.4 155,625 + GFF1484 0.71 +0.6 155,626 - GFF1484 0.71 +0.2 155,628 - GFF1484 0.72 +0.0 155,675 + GFF1484 0.78 +0.8 155,676 - GFF1484 0.78 +0.8 155,676 - GFF1484 0.78 +0.5 155,676 - GFF1484 0.78 +1.3 155,861 + -0.5 155,870 + -0.7 155,870 + +0.2 155,870 + +0.1 155,871 - +0.4 155,915 + +2.1 155,921 + +0.1 155,968 - +1.4 156,057 - GFF1485 0.23 +0.7
Or see this region's nucleotide sequence