Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1462 and GFF1463 are separated by 354 nucleotides GFF1463 and GFF1464 are separated by 257 nucleotides GFF1464 and GFF1465 are separated by 151 nucleotides
GFF1462 - Formate dehydrogenase H (EC 1.2.1.2) @ selenocysteine-containing, at 125,481 to 127,628
GFF1462
GFF1463 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 127,983 to 128,891
GFF1463
GFF1464 - PhnO protein, at 129,149 to 129,583
GFF1464
GFF1465 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 129,735 to 130,178
GFF1465
Position (kb) 127
128
129 Strain fitness (log2 ratio) -1
0
1
2
3 at 127.083 kb on - strand, within GFF1462 at 127.122 kb on + strand, within GFF1462 at 127.148 kb on + strand, within GFF1462 at 127.149 kb on - strand, within GFF1462 at 127.149 kb on - strand, within GFF1462 at 127.236 kb on - strand, within GFF1462 at 127.276 kb on + strand, within GFF1462 at 127.277 kb on - strand, within GFF1462 at 127.277 kb on - strand, within GFF1462 at 127.277 kb on - strand, within GFF1462 at 127.277 kb on - strand, within GFF1462 at 127.373 kb on - strand, within GFF1462 at 127.489 kb on + strand at 127.492 kb on + strand at 127.600 kb on + strand at 127.808 kb on + strand at 127.809 kb on - strand at 127.900 kb on + strand at 127.901 kb on - strand at 127.941 kb on + strand at 128.005 kb on - strand at 128.255 kb on + strand, within GFF1463 at 128.255 kb on + strand, within GFF1463 at 128.255 kb on + strand, within GFF1463 at 128.256 kb on - strand, within GFF1463 at 128.293 kb on + strand, within GFF1463 at 128.333 kb on + strand, within GFF1463 at 128.403 kb on + strand, within GFF1463 at 128.480 kb on + strand, within GFF1463 at 128.481 kb on - strand, within GFF1463 at 128.594 kb on + strand, within GFF1463 at 128.631 kb on + strand, within GFF1463 at 128.632 kb on - strand, within GFF1463 at 128.649 kb on + strand, within GFF1463 at 128.765 kb on + strand, within GFF1463 at 128.766 kb on - strand, within GFF1463 at 128.766 kb on - strand, within GFF1463 at 129.044 kb on + strand at 129.113 kb on + strand at 129.118 kb on + strand at 129.125 kb on + strand at 129.226 kb on + strand, within GFF1464 at 129.249 kb on - strand, within GFF1464 at 129.388 kb on + strand, within GFF1464 at 129.389 kb on - strand, within GFF1464 at 129.512 kb on + strand, within GFF1464 at 129.523 kb on + strand, within GFF1464 at 129.640 kb on + strand at 129.654 kb on - strand at 129.654 kb on - strand at 129.659 kb on - strand at 129.659 kb on - strand at 129.736 kb on + strand at 129.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 127,083 - GFF1462 0.75 +0.2 127,122 + GFF1462 0.76 -0.3 127,148 + GFF1462 0.78 -0.1 127,149 - GFF1462 0.78 +0.5 127,149 - GFF1462 0.78 +1.0 127,236 - GFF1462 0.82 -0.1 127,276 + GFF1462 0.84 -0.5 127,277 - GFF1462 0.84 -1.3 127,277 - GFF1462 0.84 +0.5 127,277 - GFF1462 0.84 -0.3 127,277 - GFF1462 0.84 +0.1 127,373 - GFF1462 0.88 -1.6 127,489 + -0.5 127,492 + +0.3 127,600 + -0.1 127,808 + +0.7 127,809 - +0.0 127,900 + +0.3 127,901 - +0.1 127,941 + +0.2 128,005 - -0.3 128,255 + GFF1463 0.30 +0.0 128,255 + GFF1463 0.30 -0.4 128,255 + GFF1463 0.30 -0.3 128,256 - GFF1463 0.30 -0.3 128,293 + GFF1463 0.34 -0.4 128,333 + GFF1463 0.39 -0.2 128,403 + GFF1463 0.46 +1.1 128,480 + GFF1463 0.55 +0.2 128,481 - GFF1463 0.55 +0.5 128,594 + GFF1463 0.67 +3.6 128,631 + GFF1463 0.71 +0.0 128,632 - GFF1463 0.71 -0.3 128,649 + GFF1463 0.73 -0.5 128,765 + GFF1463 0.86 +0.4 128,766 - GFF1463 0.86 +1.1 128,766 - GFF1463 0.86 +0.4 129,044 + +0.1 129,113 + -1.2 129,118 + +0.5 129,125 + +0.3 129,226 + GFF1464 0.18 -0.3 129,249 - GFF1464 0.23 -0.2 129,388 + GFF1464 0.55 +0.2 129,389 - GFF1464 0.55 +1.6 129,512 + GFF1464 0.83 +0.9 129,523 + GFF1464 0.86 -0.1 129,640 + -0.2 129,654 - +0.5 129,654 - +0.8 129,659 - -0.5 129,659 - -1.5 129,736 + -0.2 129,764 + -0.6
Or see this region's nucleotide sequence