Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1450 and GFF1451 overlap by 4 nucleotides GFF1451 and GFF1452 are separated by 245 nucleotides
GFF1450 - Acetate permease ActP (cation/acetate symporter), at 111,639 to 113,288
GFF1450
GFF1451 - Putative membrane protein, clustering with ActP, at 113,285 to 113,599
GFF1451
GFF1452 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 113,845 to 115,803
GFF1452
Position (kb) 113
114 Strain fitness (log2 ratio) -1
0
1 at 112.350 kb on - strand, within GFF1450 at 112.364 kb on - strand, within GFF1450 at 112.705 kb on + strand, within GFF1450 at 112.705 kb on + strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.747 kb on + strand, within GFF1450 at 112.748 kb on - strand, within GFF1450 at 112.807 kb on + strand, within GFF1450 at 112.822 kb on - strand, within GFF1450 at 112.822 kb on - strand, within GFF1450 at 112.873 kb on + strand, within GFF1450 at 112.874 kb on - strand, within GFF1450 at 112.895 kb on + strand, within GFF1450 at 112.897 kb on + strand, within GFF1450 at 112.972 kb on + strand, within GFF1450 at 112.998 kb on - strand, within GFF1450 at 113.039 kb on + strand, within GFF1450 at 113.040 kb on - strand, within GFF1450 at 113.041 kb on + strand, within GFF1450 at 113.041 kb on + strand, within GFF1450 at 113.042 kb on - strand, within GFF1450 at 113.082 kb on - strand, within GFF1450 at 113.099 kb on - strand, within GFF1450 at 113.099 kb on - strand, within GFF1450 at 113.355 kb on + strand, within GFF1451 at 113.355 kb on + strand, within GFF1451 at 113.491 kb on - strand, within GFF1451 at 113.574 kb on - strand at 113.677 kb on - strand at 113.690 kb on + strand at 113.840 kb on + strand at 113.840 kb on + strand at 113.840 kb on + strand at 113.841 kb on - strand at 113.973 kb on + strand at 114.051 kb on + strand, within GFF1452 at 114.051 kb on + strand, within GFF1452 at 114.052 kb on - strand, within GFF1452 at 114.056 kb on - strand, within GFF1452 at 114.119 kb on + strand, within GFF1452 at 114.119 kb on + strand, within GFF1452 at 114.157 kb on - strand, within GFF1452 at 114.245 kb on - strand, within GFF1452 at 114.317 kb on + strand, within GFF1452 at 114.322 kb on - strand, within GFF1452 at 114.328 kb on - strand, within GFF1452 at 114.334 kb on - strand, within GFF1452 at 114.541 kb on - strand, within GFF1452
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 112,350 - GFF1450 0.43 -0.2 112,364 - GFF1450 0.44 -0.1 112,705 + GFF1450 0.65 +0.4 112,705 + GFF1450 0.65 -0.0 112,706 - GFF1450 0.65 +0.2 112,706 - GFF1450 0.65 +0.6 112,706 - GFF1450 0.65 -0.2 112,706 - GFF1450 0.65 +1.3 112,747 + GFF1450 0.67 -0.4 112,748 - GFF1450 0.67 +0.4 112,807 + GFF1450 0.71 +0.1 112,822 - GFF1450 0.72 +0.1 112,822 - GFF1450 0.72 -0.8 112,873 + GFF1450 0.75 -0.1 112,874 - GFF1450 0.75 +1.0 112,895 + GFF1450 0.76 -0.2 112,897 + GFF1450 0.76 -0.0 112,972 + GFF1450 0.81 +0.3 112,998 - GFF1450 0.82 -0.0 113,039 + GFF1450 0.85 +0.0 113,040 - GFF1450 0.85 -0.3 113,041 + GFF1450 0.85 -0.7 113,041 + GFF1450 0.85 +0.7 113,042 - GFF1450 0.85 +0.4 113,082 - GFF1450 0.87 +0.3 113,099 - GFF1450 0.88 +0.3 113,099 - GFF1450 0.88 +0.0 113,355 + GFF1451 0.22 -0.1 113,355 + GFF1451 0.22 -0.4 113,491 - GFF1451 0.65 +0.1 113,574 - +0.2 113,677 - -0.1 113,690 + +0.4 113,840 + -0.2 113,840 + +0.5 113,840 + -0.5 113,841 - -1.4 113,973 + -0.7 114,051 + GFF1452 0.11 +0.2 114,051 + GFF1452 0.11 -0.2 114,052 - GFF1452 0.11 +0.4 114,056 - GFF1452 0.11 -0.1 114,119 + GFF1452 0.14 -0.3 114,119 + GFF1452 0.14 +0.2 114,157 - GFF1452 0.16 +0.0 114,245 - GFF1452 0.20 -0.3 114,317 + GFF1452 0.24 -0.3 114,322 - GFF1452 0.24 -0.3 114,328 - GFF1452 0.25 +0.5 114,334 - GFF1452 0.25 +0.3 114,541 - GFF1452 0.36 +0.4
Or see this region's nucleotide sequence