Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1163 and GFF1164 overlap by 8 nucleotides GFF1164 and GFF1165 overlap by 4 nucleotides GFF1165 and GFF1166 are separated by 0 nucleotides GFF1166 and GFF1167 are separated by 15 nucleotides GFF1167 and GFF1168 are separated by 55 nucleotides GFF1168 and GFF1169 are separated by 41 nucleotides GFF1169 and GFF1170 overlap by 4 nucleotides
GFF1163 - IncF plasmid conjugative transfer protein TraR, at 13,800 to 14,021
GFF1163
GFF1164 - Conjugative transfer protein PSLT087, at 14,014 to 14,481
GFF1164
GFF1165 - hypothetical protein, at 14,478 to 14,597
GFF1165
GFF1166 - FIG01045198: hypothetical protein, at 14,598 to 14,861
GFF1166
GFF1167 - IncF plasmid conjugative transfer pilus assembly protein TraC, at 14,877 to 17,525
GFF1167
GFF1168 - IncF plasmid conjugative transfer protein TrbI, at 17,581 to 17,886
GFF1168
GFF1169 - IncF plasmid conjugative transfer pilus assembly protein TraW, at 17,928 to 18,515
GFF1169
GFF1170 - IncF plasmid conjugative transfer pilus assembly protein TraU, at 18,512 to 19,504
GFF1170
Position (kb)
14
15
16
17
18 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 14.019 kb on + strand at 14.055 kb on + strand at 14.151 kb on - strand, within GFF1164 at 14.224 kb on - strand, within GFF1164 at 14.245 kb on + strand, within GFF1164 at 14.246 kb on - strand, within GFF1164 at 14.247 kb on + strand, within GFF1164 at 14.298 kb on + strand, within GFF1164 at 14.401 kb on - strand, within GFF1164 at 14.554 kb on - strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.565 kb on - strand, within GFF1165 at 14.605 kb on + strand at 14.761 kb on - strand, within GFF1166 at 15.024 kb on - strand at 15.068 kb on + strand at 15.204 kb on - strand, within GFF1167 at 15.204 kb on - strand, within GFF1167 at 15.204 kb on - strand, within GFF1167 at 15.276 kb on + strand, within GFF1167 at 15.276 kb on + strand, within GFF1167 at 15.279 kb on + strand, within GFF1167 at 15.279 kb on + strand, within GFF1167 at 15.280 kb on - strand, within GFF1167 at 15.456 kb on + strand, within GFF1167 at 15.456 kb on + strand, within GFF1167 at 15.456 kb on + strand, within GFF1167 at 15.457 kb on - strand, within GFF1167 at 15.457 kb on - strand, within GFF1167 at 15.515 kb on + strand, within GFF1167 at 15.579 kb on + strand, within GFF1167 at 15.579 kb on + strand, within GFF1167 at 15.735 kb on + strand, within GFF1167 at 15.736 kb on - strand, within GFF1167 at 15.736 kb on - strand, within GFF1167 at 15.843 kb on + strand, within GFF1167 at 15.844 kb on - strand, within GFF1167 at 15.844 kb on - strand, within GFF1167 at 15.876 kb on + strand, within GFF1167 at 16.097 kb on + strand, within GFF1167 at 16.098 kb on - strand, within GFF1167 at 16.098 kb on - strand, within GFF1167 at 16.283 kb on + strand, within GFF1167 at 16.315 kb on - strand, within GFF1167 at 16.363 kb on + strand, within GFF1167 at 16.432 kb on - strand, within GFF1167 at 16.432 kb on - strand, within GFF1167 at 16.465 kb on - strand, within GFF1167 at 16.506 kb on - strand, within GFF1167 at 16.535 kb on + strand, within GFF1167 at 16.653 kb on - strand, within GFF1167 at 16.788 kb on + strand, within GFF1167 at 16.788 kb on + strand, within GFF1167 at 16.901 kb on + strand, within GFF1167 at 16.906 kb on - strand, within GFF1167 at 16.923 kb on + strand, within GFF1167 at 17.025 kb on + strand, within GFF1167 at 17.056 kb on - strand, within GFF1167 at 17.057 kb on + strand, within GFF1167 at 17.058 kb on - strand, within GFF1167 at 17.076 kb on - strand, within GFF1167 at 17.129 kb on + strand, within GFF1167 at 17.130 kb on - strand, within GFF1167 at 17.340 kb on + strand at 17.341 kb on - strand at 17.371 kb on + strand at 17.372 kb on - strand at 17.372 kb on - strand at 17.372 kb on - strand at 17.499 kb on + strand at 17.500 kb on - strand at 17.500 kb on - strand at 17.505 kb on + strand at 17.506 kb on - strand at 17.588 kb on + strand at 17.609 kb on - strand at 17.739 kb on + strand, within GFF1168 at 17.868 kb on + strand at 17.869 kb on - strand at 18.231 kb on + strand, within GFF1169 at 18.231 kb on + strand, within GFF1169 at 18.231 kb on + strand, within GFF1169 at 18.232 kb on - strand, within GFF1169 at 18.255 kb on + strand, within GFF1169 at 18.333 kb on - strand, within GFF1169
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 14,019 + -1.2 14,055 + -0.2 14,151 - GFF1164 0.29 -0.1 14,224 - GFF1164 0.45 -0.2 14,245 + GFF1164 0.49 +0.6 14,246 - GFF1164 0.50 -0.4 14,247 + GFF1164 0.50 +1.0 14,298 + GFF1164 0.61 -0.6 14,401 - GFF1164 0.83 -0.5 14,554 - GFF1165 0.63 -0.7 14,564 + GFF1165 0.72 -0.4 14,564 + GFF1165 0.72 +0.1 14,564 + GFF1165 0.72 -0.2 14,565 - GFF1165 0.72 -0.2 14,605 + +1.4 14,761 - GFF1166 0.62 +0.6 15,024 - -0.7 15,068 + -0.4 15,204 - GFF1167 0.12 +2.2 15,204 - GFF1167 0.12 +0.2 15,204 - GFF1167 0.12 -0.9 15,276 + GFF1167 0.15 +0.5 15,276 + GFF1167 0.15 +0.6 15,279 + GFF1167 0.15 -0.0 15,279 + GFF1167 0.15 +0.5 15,280 - GFF1167 0.15 +1.3 15,456 + GFF1167 0.22 +0.2 15,456 + GFF1167 0.22 -1.2 15,456 + GFF1167 0.22 +0.7 15,457 - GFF1167 0.22 -0.4 15,457 - GFF1167 0.22 +0.9 15,515 + GFF1167 0.24 +0.1 15,579 + GFF1167 0.27 +0.5 15,579 + GFF1167 0.27 +0.1 15,735 + GFF1167 0.32 -0.2 15,736 - GFF1167 0.32 -0.7 15,736 - GFF1167 0.32 +0.3 15,843 + GFF1167 0.36 +0.5 15,844 - GFF1167 0.37 -0.0 15,844 - GFF1167 0.37 +0.1 15,876 + GFF1167 0.38 +2.2 16,097 + GFF1167 0.46 +0.4 16,098 - GFF1167 0.46 -0.2 16,098 - GFF1167 0.46 +0.6 16,283 + GFF1167 0.53 -0.9 16,315 - GFF1167 0.54 -0.2 16,363 + GFF1167 0.56 +0.7 16,432 - GFF1167 0.59 +0.5 16,432 - GFF1167 0.59 -1.2 16,465 - GFF1167 0.60 -0.5 16,506 - GFF1167 0.61 +0.6 16,535 + GFF1167 0.63 +0.1 16,653 - GFF1167 0.67 +0.7 16,788 + GFF1167 0.72 +1.1 16,788 + GFF1167 0.72 +0.1 16,901 + GFF1167 0.76 -0.5 16,906 - GFF1167 0.77 -1.6 16,923 + GFF1167 0.77 +0.4 17,025 + GFF1167 0.81 -0.9 17,056 - GFF1167 0.82 -1.9 17,057 + GFF1167 0.82 -0.2 17,058 - GFF1167 0.82 -1.0 17,076 - GFF1167 0.83 +0.6 17,129 + GFF1167 0.85 -0.2 17,130 - GFF1167 0.85 +1.1 17,340 + +0.5 17,341 - +0.7 17,371 + -0.5 17,372 - +0.6 17,372 - +1.9 17,372 - -0.0 17,499 + +0.4 17,500 - -0.4 17,500 - -0.3 17,505 + +0.8 17,506 - +1.0 17,588 + +0.6 17,609 - +1.9 17,739 + GFF1168 0.52 +0.0 17,868 + +0.4 17,869 - +1.0 18,231 + GFF1169 0.52 +0.6 18,231 + GFF1169 0.52 +0.2 18,231 + GFF1169 0.52 -0.2 18,232 - GFF1169 0.52 -0.5 18,255 + GFF1169 0.56 +0.0 18,333 - GFF1169 0.69 +0.2
Or see this region's nucleotide sequence