Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1112 and GFF1113 are separated by 45 nucleotides GFF1113 and GFF1114 are separated by 98 nucleotides
GFF1112 - Possible sulfatase, at 296,123 to 297,841
GFF1112
GFF1113 - '5'-nucleotidase (EC 3.1.3.5)' transl_table=11, at 297,887 to 299,458
GFF1113
GFF1114 - FIG01200701: possible membrane protein, at 299,557 to 300,318
GFF1114
Position (kb) 297
298
299
300 Strain fitness (log2 ratio) -1
0
1
2 at 296.895 kb on - strand, within GFF1112 at 297.052 kb on - strand, within GFF1112 at 297.074 kb on - strand, within GFF1112 at 297.088 kb on + strand, within GFF1112 at 297.222 kb on - strand, within GFF1112 at 297.225 kb on + strand, within GFF1112 at 297.226 kb on - strand, within GFF1112 at 297.226 kb on - strand, within GFF1112 at 297.226 kb on - strand, within GFF1112 at 297.269 kb on - strand, within GFF1112 at 297.326 kb on - strand, within GFF1112 at 297.326 kb on - strand, within GFF1112 at 297.472 kb on + strand, within GFF1112 at 297.533 kb on - strand, within GFF1112 at 297.550 kb on + strand, within GFF1112 at 297.558 kb on + strand, within GFF1112 at 297.619 kb on + strand, within GFF1112 at 297.620 kb on - strand, within GFF1112 at 297.710 kb on - strand at 297.843 kb on + strand at 297.843 kb on + strand at 297.843 kb on + strand at 297.844 kb on - strand at 297.844 kb on - strand at 297.847 kb on + strand at 297.852 kb on + strand at 297.876 kb on - strand at 297.889 kb on - strand at 297.889 kb on - strand at 297.896 kb on - strand at 297.896 kb on - strand at 297.899 kb on - strand at 297.951 kb on + strand at 297.952 kb on - strand at 297.952 kb on - strand at 297.952 kb on - strand at 298.063 kb on - strand, within GFF1113 at 298.070 kb on - strand, within GFF1113 at 298.070 kb on - strand, within GFF1113 at 298.141 kb on - strand, within GFF1113 at 298.163 kb on + strand, within GFF1113 at 298.267 kb on + strand, within GFF1113 at 298.268 kb on - strand, within GFF1113 at 298.285 kb on - strand, within GFF1113 at 298.285 kb on - strand, within GFF1113 at 298.292 kb on - strand, within GFF1113 at 298.295 kb on - strand, within GFF1113 at 298.312 kb on + strand, within GFF1113 at 298.332 kb on + strand, within GFF1113 at 298.333 kb on - strand, within GFF1113 at 298.333 kb on - strand, within GFF1113 at 298.517 kb on - strand, within GFF1113 at 298.518 kb on + strand, within GFF1113 at 298.518 kb on + strand, within GFF1113 at 298.519 kb on - strand, within GFF1113 at 298.530 kb on + strand, within GFF1113 at 298.547 kb on - strand, within GFF1113 at 298.555 kb on - strand, within GFF1113 at 298.555 kb on - strand, within GFF1113 at 298.555 kb on - strand, within GFF1113 at 298.600 kb on + strand, within GFF1113 at 298.601 kb on - strand, within GFF1113 at 298.604 kb on + strand, within GFF1113 at 298.617 kb on + strand, within GFF1113 at 298.618 kb on - strand, within GFF1113 at 298.702 kb on + strand, within GFF1113 at 298.703 kb on - strand, within GFF1113 at 298.923 kb on + strand, within GFF1113 at 298.945 kb on + strand, within GFF1113 at 299.012 kb on - strand, within GFF1113 at 299.307 kb on + strand at 299.340 kb on - strand at 299.863 kb on - strand, within GFF1114 at 299.991 kb on - strand, within GFF1114
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 296,895 - GFF1112 0.45 +0.3 297,052 - GFF1112 0.54 -0.8 297,074 - GFF1112 0.55 -0.2 297,088 + GFF1112 0.56 -0.8 297,222 - GFF1112 0.64 +1.2 297,225 + GFF1112 0.64 -0.1 297,226 - GFF1112 0.64 -0.2 297,226 - GFF1112 0.64 -0.5 297,226 - GFF1112 0.64 -0.1 297,269 - GFF1112 0.67 +1.5 297,326 - GFF1112 0.70 -0.4 297,326 - GFF1112 0.70 +0.1 297,472 + GFF1112 0.78 -0.8 297,533 - GFF1112 0.82 -0.4 297,550 + GFF1112 0.83 +0.4 297,558 + GFF1112 0.83 +0.8 297,619 + GFF1112 0.87 +1.6 297,620 - GFF1112 0.87 -1.4 297,710 - -0.1 297,843 + +0.2 297,843 + +0.7 297,843 + +0.0 297,844 - -0.4 297,844 - +0.1 297,847 + -0.3 297,852 + -0.2 297,876 - +2.2 297,889 - -0.2 297,889 - +0.1 297,896 - -0.5 297,896 - +0.3 297,899 - +0.2 297,951 + +1.8 297,952 - -0.9 297,952 - -0.1 297,952 - +0.9 298,063 - GFF1113 0.11 +0.6 298,070 - GFF1113 0.12 -0.3 298,070 - GFF1113 0.12 +0.3 298,141 - GFF1113 0.16 +0.7 298,163 + GFF1113 0.18 -0.4 298,267 + GFF1113 0.24 +0.9 298,268 - GFF1113 0.24 +0.4 298,285 - GFF1113 0.25 +0.2 298,285 - GFF1113 0.25 -0.1 298,292 - GFF1113 0.26 +0.0 298,295 - GFF1113 0.26 +2.2 298,312 + GFF1113 0.27 -0.2 298,332 + GFF1113 0.28 -0.4 298,333 - GFF1113 0.28 -1.0 298,333 - GFF1113 0.28 +0.4 298,517 - GFF1113 0.40 +0.5 298,518 + GFF1113 0.40 -1.0 298,518 + GFF1113 0.40 -1.1 298,519 - GFF1113 0.40 -0.8 298,530 + GFF1113 0.41 +2.2 298,547 - GFF1113 0.42 +0.4 298,555 - GFF1113 0.42 +2.6 298,555 - GFF1113 0.42 +1.2 298,555 - GFF1113 0.42 -0.2 298,600 + GFF1113 0.45 +1.0 298,601 - GFF1113 0.45 -0.2 298,604 + GFF1113 0.46 -1.3 298,617 + GFF1113 0.46 +0.1 298,618 - GFF1113 0.47 -0.1 298,702 + GFF1113 0.52 +1.5 298,703 - GFF1113 0.52 -0.5 298,923 + GFF1113 0.66 +1.2 298,945 + GFF1113 0.67 -0.3 299,012 - GFF1113 0.72 +0.3 299,307 + +0.0 299,340 - -0.2 299,863 - GFF1114 0.40 -0.4 299,991 - GFF1114 0.57 -0.2
Or see this region's nucleotide sequence