Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1035 and GFF1036 are separated by 191 nucleotides GFF1036 and GFF1037 are separated by 75 nucleotides GFF1037 and GFF1038 are separated by 128 nucleotides GFF1038 and GFF1039 are separated by 123 nucleotides
GFF1035 - Peptide chain release factor 3, at 217,240 to 218,829
GFF1035
GFF1036 - hypothetical protein, at 219,021 to 219,155
GFF1036
GFF1037 - Osmotically inducible protein OsmY, at 219,231 to 219,848
GFF1037
GFF1038 - FIG01200701: possible membrane protein, at 219,977 to 220,138
GFF1038
GFF1039 - FIG00638703: hypothetical protein, at 220,262 to 221,335
GFF1039
Position (kb) 219
220 Strain fitness (log2 ratio) -1
0
1 at 218.283 kb on + strand, within GFF1035 at 218.626 kb on + strand, within GFF1035 at 218.626 kb on + strand, within GFF1035 at 218.694 kb on + strand at 218.701 kb on - strand at 218.742 kb on + strand at 218.742 kb on + strand at 218.746 kb on + strand at 218.747 kb on - strand at 218.747 kb on - strand at 218.747 kb on - strand at 219.040 kb on + strand, within GFF1036 at 219.067 kb on + strand, within GFF1036 at 219.073 kb on + strand, within GFF1036 at 219.203 kb on + strand at 219.215 kb on - strand at 219.215 kb on - strand at 219.217 kb on - strand at 219.236 kb on + strand at 219.236 kb on + strand at 219.381 kb on - strand, within GFF1037 at 219.462 kb on - strand, within GFF1037 at 219.614 kb on + strand, within GFF1037 at 219.846 kb on + strand at 219.941 kb on + strand at 219.955 kb on - strand at 219.958 kb on + strand at 219.958 kb on + strand at 219.977 kb on - strand at 219.977 kb on - strand at 219.995 kb on - strand, within GFF1038 at 220.015 kb on - strand, within GFF1038 at 220.052 kb on - strand, within GFF1038 at 220.157 kb on + strand at 220.158 kb on - strand at 220.220 kb on - strand at 220.295 kb on - strand at 220.400 kb on - strand, within GFF1039 at 220.415 kb on - strand, within GFF1039 at 220.517 kb on - strand, within GFF1039 at 220.664 kb on + strand, within GFF1039 at 220.664 kb on + strand, within GFF1039 at 220.665 kb on - strand, within GFF1039 at 220.666 kb on + strand, within GFF1039 at 220.667 kb on - strand, within GFF1039 at 220.820 kb on - strand, within GFF1039
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 218,283 + GFF1035 0.66 -0.5 218,626 + GFF1035 0.87 -0.6 218,626 + GFF1035 0.87 -0.2 218,694 + +0.0 218,701 - -1.2 218,742 + -1.6 218,742 + +0.2 218,746 + -0.9 218,747 - +1.6 218,747 - +0.8 218,747 - +0.7 219,040 + GFF1036 0.14 -0.5 219,067 + GFF1036 0.34 -1.1 219,073 + GFF1036 0.39 +1.0 219,203 + -0.3 219,215 - +0.9 219,215 - +0.2 219,217 - -0.2 219,236 + +0.6 219,236 + +0.1 219,381 - GFF1037 0.24 +1.4 219,462 - GFF1037 0.37 -0.2 219,614 + GFF1037 0.62 +0.3 219,846 + +0.2 219,941 + +0.3 219,955 - +0.5 219,958 + -0.3 219,958 + +0.1 219,977 - +0.5 219,977 - -0.1 219,995 - GFF1038 0.11 +0.2 220,015 - GFF1038 0.23 +0.2 220,052 - GFF1038 0.46 -0.1 220,157 + -0.2 220,158 - +0.5 220,220 - -0.0 220,295 - +0.8 220,400 - GFF1039 0.13 -0.7 220,415 - GFF1039 0.14 +0.2 220,517 - GFF1039 0.24 -0.2 220,664 + GFF1039 0.37 +0.4 220,664 + GFF1039 0.37 +0.1 220,665 - GFF1039 0.38 +0.2 220,666 + GFF1039 0.38 +0.5 220,667 - GFF1039 0.38 -0.0 220,820 - GFF1039 0.52 -0.5
Or see this region's nucleotide sequence