Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1018 and GFF1019 are separated by 282 nucleotides GFF1019 and GFF1020 are separated by 57 nucleotides GFF1020 and GFF1021 are separated by 2 nucleotides GFF1021 and GFF1022 are separated by 92 nucleotides
GFF1018 - Phosphoglycerol transferase I (EC 2.7.8.20), at 203,051 to 205,342
GFF1018
GFF1019 - Putative glycoprotein/receptor, at 205,625 to 206,086
GFF1019
GFF1020 - DNA replication protein DnaC, at 206,144 to 206,881
GFF1020
GFF1021 - Primosomal protein I, at 206,884 to 207,423
GFF1021
GFF1022 - FIG001826: putative inner membrane protein, at 207,516 to 207,989
GFF1022
Position (kb)
206
207 Strain fitness (log2 ratio)
-1
0
1
2 at 205.156 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.295 kb on + strand at 205.295 kb on + strand at 205.296 kb on - strand at 205.296 kb on - strand at 205.399 kb on + strand at 205.402 kb on + strand at 205.502 kb on + strand at 205.774 kb on + strand, within GFF1019 at 205.900 kb on + strand, within GFF1019 at 205.901 kb on - strand, within GFF1019 at 206.040 kb on - strand at 206.040 kb on - strand at 206.080 kb on + strand at 206.080 kb on + strand at 206.081 kb on - strand at 206.090 kb on - strand at 207.424 kb on - strand at 207.424 kb on - strand at 207.490 kb on - strand at 207.490 kb on - strand at 207.547 kb on - strand at 207.683 kb on - strand, within GFF1022 at 207.842 kb on + strand, within GFF1022
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 205,156 + +0.8 205,293 + +0.8 205,293 + +0.1 205,293 + +0.5 205,293 + +2.2 205,293 + +0.3 205,295 + +1.4 205,295 + +0.0 205,296 - +1.1 205,296 - +0.7 205,399 + +0.2 205,402 + +0.1 205,502 + -0.5 205,774 + GFF1019 0.32 +0.3 205,900 + GFF1019 0.60 +0.3 205,901 - GFF1019 0.60 -1.0 206,040 - +0.5 206,040 - -0.3 206,080 + +0.5 206,080 + +0.2 206,081 - -0.0 206,090 - -0.3 207,424 - -0.1 207,424 - -1.3 207,490 - -0.5 207,490 - +0.1 207,547 - -0.1 207,683 - GFF1022 0.35 -0.1 207,842 + GFF1022 0.69 -0.5
Or see this region's nucleotide sequence