Experiment: LB_plus_SM_buffer with 6F2_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1001 and GFF1002 are separated by 157 nucleotides GFF1002 and GFF1003 are separated by 197 nucleotides
GFF1001 - Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72), at 182,364 to 183,953
GFF1001
GFF1002 - Type I restriction-modification system, restriction subunit R (EC 3.1.21.3), at 184,111 to 187,620
GFF1002
GFF1003 - Mrr restriction system protein, at 187,818 to 188,732
GFF1003
Position (kb) 184
185
186
187
188 Strain fitness (log2 ratio) -1
0
1
2
3 at 183.170 kb on + strand, within GFF1001 at 183.170 kb on + strand, within GFF1001 at 183.508 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.563 kb on - strand, within GFF1001 at 183.608 kb on - strand, within GFF1001 at 183.721 kb on - strand, within GFF1001 at 184.054 kb on - strand at 184.054 kb on - strand at 184.104 kb on + strand at 184.113 kb on - strand at 184.133 kb on + strand at 184.133 kb on + strand at 184.648 kb on + strand, within GFF1002 at 184.864 kb on + strand, within GFF1002 at 184.890 kb on - strand, within GFF1002 at 184.890 kb on - strand, within GFF1002 at 184.890 kb on - strand, within GFF1002 at 184.983 kb on - strand, within GFF1002 at 185.082 kb on - strand, within GFF1002 at 185.086 kb on - strand, within GFF1002 at 185.107 kb on - strand, within GFF1002 at 185.202 kb on + strand, within GFF1002 at 185.211 kb on + strand, within GFF1002 at 185.255 kb on - strand, within GFF1002 at 185.627 kb on + strand, within GFF1002 at 185.718 kb on - strand, within GFF1002 at 185.913 kb on + strand, within GFF1002 at 185.913 kb on + strand, within GFF1002 at 185.914 kb on - strand, within GFF1002 at 186.172 kb on + strand, within GFF1002 at 186.284 kb on + strand, within GFF1002 at 186.285 kb on - strand, within GFF1002 at 186.285 kb on - strand, within GFF1002 at 186.441 kb on + strand, within GFF1002 at 186.549 kb on - strand, within GFF1002 at 186.724 kb on + strand, within GFF1002 at 186.724 kb on + strand, within GFF1002 at 186.728 kb on + strand, within GFF1002 at 186.762 kb on + strand, within GFF1002 at 186.763 kb on - strand, within GFF1002 at 186.830 kb on - strand, within GFF1002 at 186.950 kb on + strand, within GFF1002 at 186.951 kb on - strand, within GFF1002 at 187.527 kb on + strand at 187.528 kb on - strand at 187.571 kb on + strand at 187.789 kb on + strand at 187.790 kb on - strand at 187.795 kb on - strand at 187.834 kb on - strand at 188.186 kb on - strand, within GFF1003 at 188.344 kb on + strand, within GFF1003 at 188.344 kb on + strand, within GFF1003 at 188.345 kb on - strand, within GFF1003 at 188.432 kb on + strand, within GFF1003 at 188.432 kb on + strand, within GFF1003 at 188.524 kb on - strand, within GFF1003 at 188.524 kb on - strand, within GFF1003 at 188.602 kb on - strand, within GFF1003 at 188.602 kb on - strand, within GFF1003 at 188.607 kb on - strand, within GFF1003
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 1.875 MOI remove 183,170 + GFF1001 0.51 -0.7 183,170 + GFF1001 0.51 +0.4 183,508 + GFF1001 0.72 -0.2 183,562 + GFF1001 0.75 +0.3 183,562 + GFF1001 0.75 +0.7 183,562 + GFF1001 0.75 -1.4 183,562 + GFF1001 0.75 +0.2 183,563 - GFF1001 0.75 -1.1 183,608 - GFF1001 0.78 +1.0 183,721 - GFF1001 0.85 -0.1 184,054 - +0.3 184,054 - +0.8 184,104 + -0.5 184,113 - +0.9 184,133 + +0.2 184,133 + +0.8 184,648 + GFF1002 0.15 +0.1 184,864 + GFF1002 0.21 +0.3 184,890 - GFF1002 0.22 +0.5 184,890 - GFF1002 0.22 +0.1 184,890 - GFF1002 0.22 +0.7 184,983 - GFF1002 0.25 -0.1 185,082 - GFF1002 0.28 +1.4 185,086 - GFF1002 0.28 +0.5 185,107 - GFF1002 0.28 -0.2 185,202 + GFF1002 0.31 -0.2 185,211 + GFF1002 0.31 +0.2 185,255 - GFF1002 0.33 +2.6 185,627 + GFF1002 0.43 +0.1 185,718 - GFF1002 0.46 +0.9 185,913 + GFF1002 0.51 +0.4 185,913 + GFF1002 0.51 -1.4 185,914 - GFF1002 0.51 -0.1 186,172 + GFF1002 0.59 -0.1 186,284 + GFF1002 0.62 +1.2 186,285 - GFF1002 0.62 +0.5 186,285 - GFF1002 0.62 +0.4 186,441 + GFF1002 0.66 +0.4 186,549 - GFF1002 0.69 +0.3 186,724 + GFF1002 0.74 +1.5 186,724 + GFF1002 0.74 +0.7 186,728 + GFF1002 0.75 +0.4 186,762 + GFF1002 0.76 -0.1 186,763 - GFF1002 0.76 +0.3 186,830 - GFF1002 0.77 +1.7 186,950 + GFF1002 0.81 -0.6 186,951 - GFF1002 0.81 +1.2 187,527 + +0.1 187,528 - -0.2 187,571 + +0.1 187,789 + -0.3 187,790 - -0.5 187,795 - +0.0 187,834 - +1.3 188,186 - GFF1003 0.40 +0.8 188,344 + GFF1003 0.57 -0.4 188,344 + GFF1003 0.57 -0.6 188,345 - GFF1003 0.58 +3.5 188,432 + GFF1003 0.67 +1.3 188,432 + GFF1003 0.67 -0.5 188,524 - GFF1003 0.77 +1.9 188,524 - GFF1003 0.77 -0.2 188,602 - GFF1003 0.86 +0.6 188,602 - GFF1003 0.86 +0.2 188,607 - GFF1003 0.86 +0.4
Or see this region's nucleotide sequence