Experiment: R2A with m-arsenite 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5436 and Atu5437 are separated by 311 nucleotides Atu5437 and Atu5438 are separated by 81 nucleotides Atu5438 and Atu5439 are separated by 250 nucleotides
Atu5436: Atu5436 - myo-inositol catabolism protein, at 429,568 to 430,470
Atu5436
Atu5437: Atu5437 - hypothetical protein, at 430,782 to 431,174
Atu5437
Atu5438: Atu5438 - 3-oxoacyl-ACP reductase, at 431,256 to 431,987
Atu5438
Atu5439: Atu5439 - hypothetical protein, at 432,238 to 432,804
Atu5439
Position (kb) 431
432 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 430.260 kb on - strand, within Atu5436 at 430.314 kb on + strand, within Atu5436 at 430.375 kb on + strand, within Atu5436 at 430.428 kb on + strand at 430.479 kb on - strand at 430.591 kb on + strand at 430.650 kb on - strand at 430.660 kb on - strand at 430.664 kb on + strand at 430.698 kb on - strand at 430.844 kb on - strand, within Atu5437 at 430.974 kb on + strand, within Atu5437 at 430.982 kb on - strand, within Atu5437 at 430.986 kb on + strand, within Atu5437 at 431.053 kb on - strand, within Atu5437 at 431.053 kb on - strand, within Atu5437 at 431.058 kb on - strand, within Atu5437 at 431.124 kb on + strand, within Atu5437 at 431.132 kb on - strand, within Atu5437 at 431.134 kb on - strand, within Atu5437 at 431.157 kb on - strand at 431.259 kb on + strand at 431.267 kb on - strand at 431.267 kb on - strand at 431.275 kb on + strand at 431.283 kb on - strand at 431.283 kb on - strand at 431.312 kb on - strand at 431.408 kb on - strand, within Atu5438 at 431.507 kb on + strand, within Atu5438 at 431.515 kb on - strand, within Atu5438 at 431.533 kb on - strand, within Atu5438 at 431.533 kb on - strand, within Atu5438 at 431.533 kb on - strand, within Atu5438 at 431.554 kb on + strand, within Atu5438 at 431.562 kb on - strand, within Atu5438 at 431.581 kb on + strand, within Atu5438 at 431.608 kb on - strand, within Atu5438 at 431.749 kb on - strand, within Atu5438 at 431.785 kb on + strand, within Atu5438 at 431.785 kb on + strand, within Atu5438 at 431.797 kb on + strand, within Atu5438 at 431.805 kb on - strand, within Atu5438 at 431.915 kb on - strand at 431.942 kb on + strand at 431.979 kb on - strand at 431.990 kb on - strand at 432.011 kb on + strand at 432.016 kb on + strand at 432.019 kb on - strand at 432.019 kb on - strand at 432.022 kb on + strand at 432.029 kb on - strand at 432.090 kb on + strand at 432.132 kb on - strand at 432.211 kb on - strand at 432.218 kb on - strand at 432.237 kb on + strand at 432.249 kb on - strand at 432.271 kb on - strand at 432.338 kb on - strand, within Atu5439 at 432.363 kb on + strand, within Atu5439 at 432.363 kb on + strand, within Atu5439 at 432.368 kb on + strand, within Atu5439 at 432.371 kb on - strand, within Atu5439 at 432.371 kb on - strand, within Atu5439 at 432.373 kb on + strand, within Atu5439 at 432.376 kb on - strand, within Atu5439 at 432.378 kb on + strand, within Atu5439 at 432.381 kb on - strand, within Atu5439 at 432.386 kb on - strand, within Atu5439 at 432.386 kb on - strand, within Atu5439 at 432.404 kb on + strand, within Atu5439 at 432.498 kb on - strand, within Atu5439 at 432.614 kb on - strand, within Atu5439 at 432.661 kb on + strand, within Atu5439 at 432.683 kb on + strand, within Atu5439 at 432.702 kb on - strand, within Atu5439 at 432.714 kb on - strand, within Atu5439 at 432.732 kb on - strand, within Atu5439 at 432.757 kb on + strand at 432.757 kb on - strand at 432.759 kb on + strand at 432.759 kb on + strand at 432.847 kb on - strand at 432.858 kb on + strand at 432.914 kb on + strand at 432.926 kb on + strand at 432.928 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with m-arsenite 0.8 mM remove 430,260 - Atu5436 0.77 -0.3 430,314 + Atu5436 0.83 +2.1 430,375 + Atu5436 0.89 -1.6 430,428 + -1.0 430,479 - -0.6 430,591 + -2.2 430,650 - -0.2 430,660 - -0.2 430,664 + -0.8 430,698 - +1.3 430,844 - Atu5437 0.16 -0.3 430,974 + Atu5437 0.49 -0.6 430,982 - Atu5437 0.51 -0.2 430,986 + Atu5437 0.52 +1.9 431,053 - Atu5437 0.69 -0.3 431,053 - Atu5437 0.69 -1.8 431,058 - Atu5437 0.70 -0.3 431,124 + Atu5437 0.87 +1.2 431,132 - Atu5437 0.89 +0.2 431,134 - Atu5437 0.90 -1.2 431,157 - -0.5 431,259 + -0.6 431,267 - +0.0 431,267 - -1.3 431,275 + +1.5 431,283 - +0.1 431,283 - +0.6 431,312 - +2.7 431,408 - Atu5438 0.21 +0.1 431,507 + Atu5438 0.34 -1.4 431,515 - Atu5438 0.35 -0.9 431,533 - Atu5438 0.38 +0.4 431,533 - Atu5438 0.38 +0.0 431,533 - Atu5438 0.38 -0.4 431,554 + Atu5438 0.41 -0.3 431,562 - Atu5438 0.42 +0.3 431,581 + Atu5438 0.44 -0.8 431,608 - Atu5438 0.48 +1.9 431,749 - Atu5438 0.67 -1.9 431,785 + Atu5438 0.72 -1.2 431,785 + Atu5438 0.72 -0.9 431,797 + Atu5438 0.74 -1.6 431,805 - Atu5438 0.75 -0.2 431,915 - +0.5 431,942 + -0.2 431,979 - -0.4 431,990 - +0.6 432,011 + +2.9 432,016 + +0.3 432,019 - +0.3 432,019 - +1.7 432,022 + +1.1 432,029 - -0.8 432,090 + -0.4 432,132 - -1.7 432,211 - -1.4 432,218 - +1.4 432,237 + +1.0 432,249 - +0.1 432,271 - +1.3 432,338 - Atu5439 0.18 +1.1 432,363 + Atu5439 0.22 +1.0 432,363 + Atu5439 0.22 +0.9 432,368 + Atu5439 0.23 +0.9 432,371 - Atu5439 0.23 -0.1 432,371 - Atu5439 0.23 +0.1 432,373 + Atu5439 0.24 -0.4 432,376 - Atu5439 0.24 +0.4 432,378 + Atu5439 0.25 -0.5 432,381 - Atu5439 0.25 +1.4 432,386 - Atu5439 0.26 +0.5 432,386 - Atu5439 0.26 +1.6 432,404 + Atu5439 0.29 -2.2 432,498 - Atu5439 0.46 +1.2 432,614 - Atu5439 0.66 +1.5 432,661 + Atu5439 0.75 +0.6 432,683 + Atu5439 0.78 -1.1 432,702 - Atu5439 0.82 +0.5 432,714 - Atu5439 0.84 +1.1 432,732 - Atu5439 0.87 +0.0 432,757 + +0.8 432,757 - -0.0 432,759 + -0.5 432,759 + +0.0 432,847 - -0.7 432,858 + -1.1 432,914 + -0.8 432,926 + +0.4 432,928 - +1.4
Or see this region's nucleotide sequence