Experiment: R2A with m-arsenite 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4027 and Atu4028 are separated by 2 nucleotides Atu4028 and Atu4029 are separated by 151 nucleotides Atu4029 and Atu4030 are separated by 34 nucleotides Atu4030 and Atu4031 overlap by 4 nucleotides
Atu4027: Atu4027 - cytochrome c2, at 1,142,286 to 1,142,786
Atu4027
Atu4028: Atu4028 - recombination regulator RecX, at 1,142,789 to 1,143,298
Atu4028
Atu4029: Atu4029 - gluconolactonase, at 1,143,450 to 1,144,370
Atu4029
Atu4030: Atu4030 - sugar ABC transporter permease, at 1,144,405 to 1,145,352
Atu4030
Atu4031: Atu4031 - sugar ABC transporter permease, at 1,145,349 to 1,146,290
Atu4031
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1142.452 kb on + strand, within Atu4027 at 1142.642 kb on - strand, within Atu4027 at 1142.647 kb on - strand, within Atu4027 at 1142.647 kb on - strand, within Atu4027 at 1142.647 kb on - strand, within Atu4027 at 1142.787 kb on + strand at 1142.795 kb on - strand at 1143.032 kb on + strand, within Atu4028 at 1143.035 kb on - strand, within Atu4028 at 1143.057 kb on + strand, within Atu4028 at 1143.165 kb on + strand, within Atu4028 at 1143.173 kb on - strand, within Atu4028 at 1143.173 kb on - strand, within Atu4028 at 1143.178 kb on - strand, within Atu4028 at 1143.254 kb on + strand at 1143.256 kb on + strand at 1143.330 kb on - strand at 1143.413 kb on - strand at 1143.414 kb on + strand at 1143.576 kb on - strand, within Atu4029 at 1143.713 kb on + strand, within Atu4029 at 1143.713 kb on + strand, within Atu4029 at 1143.713 kb on + strand, within Atu4029 at 1143.721 kb on - strand, within Atu4029 at 1143.864 kb on - strand, within Atu4029 at 1143.901 kb on - strand, within Atu4029 at 1144.033 kb on - strand, within Atu4029 at 1144.094 kb on + strand, within Atu4029 at 1144.233 kb on + strand, within Atu4029 at 1144.475 kb on - strand at 1144.519 kb on - strand, within Atu4030 at 1144.546 kb on - strand, within Atu4030 at 1144.548 kb on - strand, within Atu4030 at 1144.639 kb on - strand, within Atu4030 at 1144.712 kb on - strand, within Atu4030 at 1144.807 kb on + strand, within Atu4030 at 1144.834 kb on + strand, within Atu4030 at 1144.992 kb on - strand, within Atu4030 at 1145.011 kb on - strand, within Atu4030 at 1145.013 kb on + strand, within Atu4030 at 1145.013 kb on + strand, within Atu4030 at 1145.013 kb on + strand, within Atu4030 at 1145.073 kb on + strand, within Atu4030 at 1145.221 kb on + strand, within Atu4030 at 1145.249 kb on - strand, within Atu4030 at 1145.287 kb on + strand at 1145.309 kb on + strand at 1145.314 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with m-arsenite 0.8 mM remove 1,142,452 + Atu4027 0.33 -0.3 1,142,642 - Atu4027 0.71 +0.1 1,142,647 - Atu4027 0.72 -0.1 1,142,647 - Atu4027 0.72 +0.5 1,142,647 - Atu4027 0.72 +0.8 1,142,787 + -1.5 1,142,795 - +0.5 1,143,032 + Atu4028 0.48 -0.3 1,143,035 - Atu4028 0.48 -0.7 1,143,057 + Atu4028 0.53 +1.1 1,143,165 + Atu4028 0.74 -0.0 1,143,173 - Atu4028 0.75 -1.1 1,143,173 - Atu4028 0.75 +0.9 1,143,178 - Atu4028 0.76 -1.2 1,143,254 + +0.0 1,143,256 + -0.8 1,143,330 - +0.5 1,143,413 - -0.1 1,143,414 + -0.5 1,143,576 - Atu4029 0.14 -0.7 1,143,713 + Atu4029 0.29 -0.7 1,143,713 + Atu4029 0.29 -2.1 1,143,713 + Atu4029 0.29 -1.3 1,143,721 - Atu4029 0.29 +0.3 1,143,864 - Atu4029 0.45 +1.1 1,143,901 - Atu4029 0.49 +0.3 1,144,033 - Atu4029 0.63 -0.1 1,144,094 + Atu4029 0.70 -2.1 1,144,233 + Atu4029 0.85 -0.4 1,144,475 - +3.7 1,144,519 - Atu4030 0.12 +1.2 1,144,546 - Atu4030 0.15 +2.2 1,144,548 - Atu4030 0.15 +0.4 1,144,639 - Atu4030 0.25 +0.6 1,144,712 - Atu4030 0.32 +0.5 1,144,807 + Atu4030 0.42 +0.8 1,144,834 + Atu4030 0.45 +0.3 1,144,992 - Atu4030 0.62 +2.1 1,145,011 - Atu4030 0.64 -0.2 1,145,013 + Atu4030 0.64 +0.2 1,145,013 + Atu4030 0.64 +1.3 1,145,013 + Atu4030 0.64 +1.1 1,145,073 + Atu4030 0.70 +0.8 1,145,221 + Atu4030 0.86 +0.6 1,145,249 - Atu4030 0.89 +1.3 1,145,287 + +0.8 1,145,309 + +1.3 1,145,314 + +0.2
Or see this region's nucleotide sequence