Experiment: R2A with m-arsenite 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3208 and Atu3210 are separated by 311 nucleotides Atu3210 and Atu3212 are separated by 287 nucleotides
Atu3208: Atu3208 - malate dehydrogenase, at 222,123 to 223,169
Atu3208
Atu3210: Atu3210 - ftsk cell division protein, at 223,481 to 226,213
Atu3210
Atu3212: Atu3212 - oligopeptide ABC transporter substrate-binding protein, at 226,501 to 228,198
Atu3212
Position (kb) 223
224
225
226
227 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 222.533 kb on - strand, within Atu3208 at 222.630 kb on - strand, within Atu3208 at 222.654 kb on - strand, within Atu3208 at 222.701 kb on + strand, within Atu3208 at 222.972 kb on + strand, within Atu3208 at 223.016 kb on - strand, within Atu3208 at 223.037 kb on + strand, within Atu3208 at 223.045 kb on - strand, within Atu3208 at 223.047 kb on - strand, within Atu3208 at 223.058 kb on + strand, within Atu3208 at 223.066 kb on - strand at 223.145 kb on + strand at 223.206 kb on + strand at 223.311 kb on + strand at 223.319 kb on - strand at 223.402 kb on + strand at 223.462 kb on + strand at 223.470 kb on - strand at 223.470 kb on - strand at 223.633 kb on + strand at 223.636 kb on - strand at 223.700 kb on + strand at 223.727 kb on + strand at 223.785 kb on - strand, within Atu3210 at 223.864 kb on - strand, within Atu3210 at 223.977 kb on - strand, within Atu3210 at 223.981 kb on + strand, within Atu3210 at 224.063 kb on - strand, within Atu3210 at 224.172 kb on + strand, within Atu3210 at 224.180 kb on - strand, within Atu3210 at 224.180 kb on - strand, within Atu3210 at 224.301 kb on - strand, within Atu3210 at 224.318 kb on - strand, within Atu3210 at 224.460 kb on - strand, within Atu3210 at 224.480 kb on - strand, within Atu3210 at 224.507 kb on + strand, within Atu3210 at 224.507 kb on + strand, within Atu3210 at 224.515 kb on - strand, within Atu3210 at 224.594 kb on - strand, within Atu3210 at 224.671 kb on - strand, within Atu3210 at 224.715 kb on + strand, within Atu3210 at 224.723 kb on - strand, within Atu3210 at 224.723 kb on - strand, within Atu3210 at 224.723 kb on - strand, within Atu3210 at 224.844 kb on + strand, within Atu3210 at 224.899 kb on - strand, within Atu3210 at 224.955 kb on - strand, within Atu3210 at 225.059 kb on - strand, within Atu3210 at 225.109 kb on + strand, within Atu3210 at 225.124 kb on - strand, within Atu3210 at 225.141 kb on + strand, within Atu3210 at 225.149 kb on - strand, within Atu3210 at 225.149 kb on - strand, within Atu3210 at 225.296 kb on + strand, within Atu3210 at 225.346 kb on + strand, within Atu3210 at 225.359 kb on - strand, within Atu3210 at 225.359 kb on - strand, within Atu3210 at 225.406 kb on - strand, within Atu3210 at 225.422 kb on + strand, within Atu3210 at 225.437 kb on + strand, within Atu3210 at 225.442 kb on + strand, within Atu3210 at 225.477 kb on + strand, within Atu3210 at 225.610 kb on + strand, within Atu3210 at 225.614 kb on + strand, within Atu3210 at 225.620 kb on - strand, within Atu3210 at 225.718 kb on + strand, within Atu3210 at 225.726 kb on - strand, within Atu3210 at 225.981 kb on - strand at 225.981 kb on - strand at 226.030 kb on - strand at 226.030 kb on - strand at 226.030 kb on - strand at 226.032 kb on - strand at 226.187 kb on + strand at 226.195 kb on - strand at 226.206 kb on + strand at 226.516 kb on - strand at 226.522 kb on + strand at 226.718 kb on + strand, within Atu3212 at 226.773 kb on - strand, within Atu3212 at 226.826 kb on - strand, within Atu3212 at 226.886 kb on + strand, within Atu3212 at 226.886 kb on + strand, within Atu3212 at 226.894 kb on - strand, within Atu3212 at 226.894 kb on - strand, within Atu3212 at 226.894 kb on - strand, within Atu3212 at 226.911 kb on - strand, within Atu3212 at 226.927 kb on + strand, within Atu3212 at 226.927 kb on + strand, within Atu3212 at 226.927 kb on + strand, within Atu3212 at 226.927 kb on + strand, within Atu3212 at 227.006 kb on - strand, within Atu3212 at 227.106 kb on + strand, within Atu3212
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with m-arsenite 0.8 mM remove 222,533 - Atu3208 0.39 +0.1 222,630 - Atu3208 0.48 +1.2 222,654 - Atu3208 0.51 +0.6 222,701 + Atu3208 0.55 +1.2 222,972 + Atu3208 0.81 -0.7 223,016 - Atu3208 0.85 -0.0 223,037 + Atu3208 0.87 +0.4 223,045 - Atu3208 0.88 -0.5 223,047 - Atu3208 0.88 +0.7 223,058 + Atu3208 0.89 +2.2 223,066 - +1.1 223,145 + +0.6 223,206 + +0.7 223,311 + -0.1 223,319 - +0.7 223,402 + +0.1 223,462 + +0.1 223,470 - +0.6 223,470 - -0.2 223,633 + +1.1 223,636 - -0.7 223,700 + -1.2 223,727 + +0.0 223,785 - Atu3210 0.11 +0.7 223,864 - Atu3210 0.14 -0.2 223,977 - Atu3210 0.18 +0.9 223,981 + Atu3210 0.18 +0.6 224,063 - Atu3210 0.21 +0.6 224,172 + Atu3210 0.25 -0.8 224,180 - Atu3210 0.26 -0.3 224,180 - Atu3210 0.26 +1.6 224,301 - Atu3210 0.30 +0.5 224,318 - Atu3210 0.31 +1.7 224,460 - Atu3210 0.36 +0.4 224,480 - Atu3210 0.37 -0.1 224,507 + Atu3210 0.38 +0.1 224,507 + Atu3210 0.38 -0.8 224,515 - Atu3210 0.38 -0.4 224,594 - Atu3210 0.41 -0.8 224,671 - Atu3210 0.44 +0.4 224,715 + Atu3210 0.45 +0.8 224,723 - Atu3210 0.45 +0.3 224,723 - Atu3210 0.45 -1.2 224,723 - Atu3210 0.45 +1.2 224,844 + Atu3210 0.50 +0.7 224,899 - Atu3210 0.52 +0.3 224,955 - Atu3210 0.54 +1.4 225,059 - Atu3210 0.58 -0.6 225,109 + Atu3210 0.60 -0.5 225,124 - Atu3210 0.60 -2.1 225,141 + Atu3210 0.61 +1.1 225,149 - Atu3210 0.61 +0.1 225,149 - Atu3210 0.61 -0.9 225,296 + Atu3210 0.66 +0.6 225,346 + Atu3210 0.68 +0.6 225,359 - Atu3210 0.69 +0.7 225,359 - Atu3210 0.69 -0.1 225,406 - Atu3210 0.70 +1.1 225,422 + Atu3210 0.71 -0.1 225,437 + Atu3210 0.72 -0.1 225,442 + Atu3210 0.72 -0.8 225,477 + Atu3210 0.73 -0.3 225,610 + Atu3210 0.78 +0.4 225,614 + Atu3210 0.78 +0.1 225,620 - Atu3210 0.78 +0.1 225,718 + Atu3210 0.82 +0.2 225,726 - Atu3210 0.82 -0.0 225,981 - -0.9 225,981 - +0.9 226,030 - +0.7 226,030 - +0.6 226,030 - +0.0 226,032 - +0.3 226,187 + -1.8 226,195 - +0.6 226,206 + -0.9 226,516 - -1.2 226,522 + +1.2 226,718 + Atu3212 0.13 -0.4 226,773 - Atu3212 0.16 -0.9 226,826 - Atu3212 0.19 -0.2 226,886 + Atu3212 0.23 -1.2 226,886 + Atu3212 0.23 +1.3 226,894 - Atu3212 0.23 +4.4 226,894 - Atu3212 0.23 +0.6 226,894 - Atu3212 0.23 -0.1 226,911 - Atu3212 0.24 -0.6 226,927 + Atu3212 0.25 -0.4 226,927 + Atu3212 0.25 +1.1 226,927 + Atu3212 0.25 +0.2 226,927 + Atu3212 0.25 +0.2 227,006 - Atu3212 0.30 +0.2 227,106 + Atu3212 0.36 -1.2
Or see this region's nucleotide sequence