Experiment: R2A with m-arsenite 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3178 and Atu3179 are separated by 28 nucleotides Atu3179 and Atu3180 overlap by 8 nucleotides Atu3180 and Atu3181 are separated by 300 nucleotides
Atu3178: Atu3178 - ABC transporter substrate binding protein, at 188,009 to 189,022
Atu3178
Atu3179: Atu3179 - ABC transporter permease, at 189,051 to 189,917
Atu3179
Atu3180: Atu3180 - ABC transporter permease, at 189,910 to 190,743
Atu3180
Atu3181: Atu3181 - hypothetical protein, at 191,044 to 192,246
Atu3181
Position (kb) 189
190
191 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 188.932 kb on - strand at 188.946 kb on + strand at 188.946 kb on + strand at 188.954 kb on - strand at 189.020 kb on - strand at 189.020 kb on - strand at 189.020 kb on - strand at 189.020 kb on - strand at 189.058 kb on + strand at 189.066 kb on - strand at 189.066 kb on - strand at 189.116 kb on - strand at 189.191 kb on - strand, within Atu3179 at 189.196 kb on - strand, within Atu3179 at 189.240 kb on + strand, within Atu3179 at 189.240 kb on + strand, within Atu3179 at 189.248 kb on - strand, within Atu3179 at 189.259 kb on + strand, within Atu3179 at 189.259 kb on + strand, within Atu3179 at 189.262 kb on - strand, within Atu3179 at 189.267 kb on - strand, within Atu3179 at 189.267 kb on - strand, within Atu3179 at 189.349 kb on - strand, within Atu3179 at 189.398 kb on - strand, within Atu3179 at 189.424 kb on - strand, within Atu3179 at 189.456 kb on - strand, within Atu3179 at 189.484 kb on + strand, within Atu3179 at 189.600 kb on + strand, within Atu3179 at 189.605 kb on + strand, within Atu3179 at 189.684 kb on + strand, within Atu3179 at 189.689 kb on + strand, within Atu3179 at 189.781 kb on - strand, within Atu3179 at 189.887 kb on - strand at 189.913 kb on + strand at 189.961 kb on + strand at 189.961 kb on + strand at 189.978 kb on + strand at 189.981 kb on - strand at 189.983 kb on + strand at 190.117 kb on - strand, within Atu3180 at 190.139 kb on - strand, within Atu3180 at 190.172 kb on - strand, within Atu3180 at 190.208 kb on + strand, within Atu3180 at 190.217 kb on - strand, within Atu3180 at 190.234 kb on + strand, within Atu3180 at 190.268 kb on + strand, within Atu3180 at 190.295 kb on - strand, within Atu3180 at 190.397 kb on + strand, within Atu3180 at 190.504 kb on + strand, within Atu3180 at 190.542 kb on + strand, within Atu3180 at 190.603 kb on - strand, within Atu3180 at 190.654 kb on + strand, within Atu3180 at 190.664 kb on + strand at 190.669 kb on + strand at 190.678 kb on + strand at 190.678 kb on + strand at 190.861 kb on + strand at 190.861 kb on + strand at 190.861 kb on + strand at 190.869 kb on - strand at 190.869 kb on - strand at 190.878 kb on + strand at 190.886 kb on - strand at 191.027 kb on - strand at 191.076 kb on - strand at 191.204 kb on + strand, within Atu3181 at 191.230 kb on - strand, within Atu3181 at 191.285 kb on + strand, within Atu3181 at 191.378 kb on + strand, within Atu3181 at 191.427 kb on + strand, within Atu3181 at 191.553 kb on + strand, within Atu3181 at 191.555 kb on - strand, within Atu3181
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with m-arsenite 0.8 mM remove 188,932 - -1.2 188,946 + -0.5 188,946 + -0.7 188,954 - -0.6 189,020 - -0.7 189,020 - +2.2 189,020 - -1.0 189,020 - -2.1 189,058 + -0.4 189,066 - -0.8 189,066 - +0.6 189,116 - -2.0 189,191 - Atu3179 0.16 +0.5 189,196 - Atu3179 0.17 -3.2 189,240 + Atu3179 0.22 -0.2 189,240 + Atu3179 0.22 -1.3 189,248 - Atu3179 0.23 -1.6 189,259 + Atu3179 0.24 -2.6 189,259 + Atu3179 0.24 -1.8 189,262 - Atu3179 0.24 -0.8 189,267 - Atu3179 0.25 -1.3 189,267 - Atu3179 0.25 -0.7 189,349 - Atu3179 0.34 -1.7 189,398 - Atu3179 0.40 -2.9 189,424 - Atu3179 0.43 -0.5 189,456 - Atu3179 0.47 -1.4 189,484 + Atu3179 0.50 -0.9 189,600 + Atu3179 0.63 -2.4 189,605 + Atu3179 0.64 -1.3 189,684 + Atu3179 0.73 +0.1 189,689 + Atu3179 0.74 -1.0 189,781 - Atu3179 0.84 -0.2 189,887 - -0.0 189,913 + -3.8 189,961 + +0.2 189,961 + -1.8 189,978 + -2.6 189,981 - -1.3 189,983 + -2.4 190,117 - Atu3180 0.25 -0.4 190,139 - Atu3180 0.27 -2.9 190,172 - Atu3180 0.31 -3.0 190,208 + Atu3180 0.36 -2.3 190,217 - Atu3180 0.37 -1.2 190,234 + Atu3180 0.39 +0.5 190,268 + Atu3180 0.43 -3.2 190,295 - Atu3180 0.46 -3.0 190,397 + Atu3180 0.58 -1.6 190,504 + Atu3180 0.71 -1.6 190,542 + Atu3180 0.76 -2.7 190,603 - Atu3180 0.83 -0.1 190,654 + Atu3180 0.89 -0.1 190,664 + +1.5 190,669 + +0.6 190,678 + +1.1 190,678 + -2.8 190,861 + -0.4 190,861 + -0.7 190,861 + -1.3 190,869 - +0.9 190,869 - -0.7 190,878 + +0.3 190,886 - -1.3 191,027 - +0.6 191,076 - +1.3 191,204 + Atu3181 0.13 -0.3 191,230 - Atu3181 0.15 -0.0 191,285 + Atu3181 0.20 -0.5 191,378 + Atu3181 0.28 -0.0 191,427 + Atu3181 0.32 -2.3 191,553 + Atu3181 0.42 +1.3 191,555 - Atu3181 0.42 -0.1
Or see this region's nucleotide sequence