Experiment: R2A with m-arsenite 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2713 and Atu2714 are separated by 19 nucleotides Atu2714 and Atu2715 are separated by 193 nucleotides Atu2715 and Atu2716 are separated by 97 nucleotides
Atu2713: Atu2713 - ABC transporter, membrane spanning protein (thiamine), at 2,697,534 to 2,699,159
Atu2713
Atu2714: Atu2714 - ABC transporter, substrate binding protein (thiamine), at 2,699,179 to 2,700,192
Atu2714
Atu2715: Atu2715 - hypothetical protein, at 2,700,386 to 2,700,778
Atu2715
Atu2716: Atu2716 - ABC transporter, nucleotide binding/ATPase protein, at 2,700,876 to 2,702,702
Atu2716
Position (kb)
2699
2700
2701 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2698.291 kb on + strand, within Atu2713 at 2698.405 kb on - strand, within Atu2713 at 2698.485 kb on + strand, within Atu2713 at 2698.492 kb on - strand, within Atu2713 at 2698.517 kb on + strand, within Atu2713 at 2698.705 kb on - strand, within Atu2713 at 2698.705 kb on - strand, within Atu2713 at 2698.790 kb on - strand, within Atu2713 at 2698.806 kb on - strand, within Atu2713 at 2698.808 kb on - strand, within Atu2713 at 2698.808 kb on - strand, within Atu2713 at 2698.808 kb on - strand, within Atu2713 at 2698.851 kb on - strand, within Atu2713 at 2698.899 kb on - strand, within Atu2713 at 2698.899 kb on - strand, within Atu2713 at 2698.989 kb on - strand, within Atu2713 at 2698.989 kb on - strand, within Atu2713 at 2698.989 kb on - strand, within Atu2713 at 2699.012 kb on + strand at 2699.012 kb on + strand at 2699.014 kb on + strand at 2699.014 kb on + strand at 2699.020 kb on - strand at 2699.020 kb on - strand at 2699.022 kb on - strand at 2699.059 kb on - strand at 2699.066 kb on - strand at 2699.165 kb on + strand at 2699.180 kb on - strand at 2699.213 kb on + strand at 2699.389 kb on - strand, within Atu2714 at 2699.492 kb on - strand, within Atu2714 at 2699.506 kb on + strand, within Atu2714 at 2699.525 kb on + strand, within Atu2714 at 2699.525 kb on + strand, within Atu2714 at 2699.525 kb on + strand, within Atu2714 at 2699.525 kb on + strand, within Atu2714 at 2699.525 kb on + strand, within Atu2714 at 2699.533 kb on - strand, within Atu2714 at 2699.533 kb on - strand, within Atu2714 at 2699.533 kb on - strand, within Atu2714 at 2699.616 kb on + strand, within Atu2714 at 2699.685 kb on + strand, within Atu2714 at 2699.685 kb on + strand, within Atu2714 at 2699.685 kb on + strand, within Atu2714 at 2699.740 kb on - strand, within Atu2714 at 2699.946 kb on + strand, within Atu2714 at 2699.975 kb on + strand, within Atu2714 at 2699.993 kb on - strand, within Atu2714 at 2700.094 kb on + strand at 2700.094 kb on + strand at 2700.108 kb on + strand at 2700.116 kb on - strand at 2700.116 kb on - strand at 2700.118 kb on + strand at 2700.126 kb on - strand at 2700.225 kb on - strand at 2700.231 kb on + strand at 2700.231 kb on + strand at 2700.231 kb on + strand at 2700.231 kb on + strand at 2700.239 kb on - strand at 2700.239 kb on - strand at 2700.239 kb on - strand at 2700.239 kb on - strand at 2700.239 kb on - strand at 2700.258 kb on + strand at 2700.314 kb on + strand at 2700.314 kb on + strand at 2700.384 kb on + strand at 2700.384 kb on + strand at 2700.384 kb on + strand at 2700.392 kb on - strand at 2700.392 kb on - strand at 2700.444 kb on - strand, within Atu2715 at 2700.518 kb on - strand, within Atu2715 at 2700.565 kb on + strand, within Atu2715 at 2700.565 kb on - strand, within Atu2715 at 2700.566 kb on - strand, within Atu2715 at 2700.660 kb on + strand, within Atu2715 at 2700.662 kb on + strand, within Atu2715 at 2700.670 kb on - strand, within Atu2715 at 2700.832 kb on + strand at 2700.851 kb on - strand at 2700.851 kb on - strand at 2700.879 kb on + strand at 2700.908 kb on + strand at 2700.916 kb on - strand at 2701.015 kb on + strand at 2701.023 kb on - strand at 2701.082 kb on - strand, within Atu2716 at 2701.111 kb on + strand, within Atu2716
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with m-arsenite 0.8 mM remove 2,698,291 + Atu2713 0.47 -1.5 2,698,405 - Atu2713 0.54 -1.6 2,698,485 + Atu2713 0.58 -1.8 2,698,492 - Atu2713 0.59 -1.1 2,698,517 + Atu2713 0.60 -2.0 2,698,705 - Atu2713 0.72 -0.5 2,698,705 - Atu2713 0.72 -1.0 2,698,790 - Atu2713 0.77 -1.0 2,698,806 - Atu2713 0.78 -1.6 2,698,808 - Atu2713 0.78 -0.4 2,698,808 - Atu2713 0.78 -1.9 2,698,808 - Atu2713 0.78 -0.5 2,698,851 - Atu2713 0.81 -1.0 2,698,899 - Atu2713 0.84 -1.5 2,698,899 - Atu2713 0.84 -0.3 2,698,989 - Atu2713 0.89 -1.0 2,698,989 - Atu2713 0.89 -2.0 2,698,989 - Atu2713 0.89 -2.6 2,699,012 + -2.6 2,699,012 + -0.2 2,699,014 + -1.6 2,699,014 + -1.7 2,699,020 - -2.0 2,699,020 - -0.2 2,699,022 - +0.1 2,699,059 - -0.6 2,699,066 - -0.6 2,699,165 + -1.6 2,699,180 - +0.2 2,699,213 + -1.7 2,699,389 - Atu2714 0.21 +1.5 2,699,492 - Atu2714 0.31 -1.2 2,699,506 + Atu2714 0.32 -1.6 2,699,525 + Atu2714 0.34 -0.8 2,699,525 + Atu2714 0.34 -3.3 2,699,525 + Atu2714 0.34 -0.0 2,699,525 + Atu2714 0.34 -1.7 2,699,525 + Atu2714 0.34 -1.2 2,699,533 - Atu2714 0.35 -2.4 2,699,533 - Atu2714 0.35 -1.9 2,699,533 - Atu2714 0.35 -1.1 2,699,616 + Atu2714 0.43 -0.8 2,699,685 + Atu2714 0.50 -1.6 2,699,685 + Atu2714 0.50 -3.7 2,699,685 + Atu2714 0.50 -1.1 2,699,740 - Atu2714 0.55 +0.7 2,699,946 + Atu2714 0.76 -0.4 2,699,975 + Atu2714 0.79 -0.9 2,699,993 - Atu2714 0.80 -0.4 2,700,094 + -3.1 2,700,094 + -2.0 2,700,108 + -2.2 2,700,116 - -0.3 2,700,116 - -0.6 2,700,118 + +0.5 2,700,126 - -1.1 2,700,225 - -2.5 2,700,231 + -2.0 2,700,231 + -2.8 2,700,231 + -1.5 2,700,231 + -0.3 2,700,239 - +0.8 2,700,239 - +0.9 2,700,239 - -0.8 2,700,239 - -1.2 2,700,239 - -1.4 2,700,258 + +1.5 2,700,314 + +0.1 2,700,314 + -0.4 2,700,384 + -0.5 2,700,384 + -0.5 2,700,384 + -1.0 2,700,392 - -2.0 2,700,392 - +0.5 2,700,444 - Atu2715 0.15 +0.9 2,700,518 - Atu2715 0.34 +0.5 2,700,565 + Atu2715 0.46 -0.2 2,700,565 - Atu2715 0.46 +0.7 2,700,566 - Atu2715 0.46 +0.4 2,700,660 + Atu2715 0.70 -0.3 2,700,662 + Atu2715 0.70 -1.0 2,700,670 - Atu2715 0.72 +0.5 2,700,832 + +0.4 2,700,851 - +0.1 2,700,851 - +0.1 2,700,879 + +0.6 2,700,908 + -1.0 2,700,916 - +0.8 2,701,015 + +0.4 2,701,023 - -2.0 2,701,082 - Atu2716 0.11 +0.3 2,701,111 + Atu2716 0.13 +0.9
Or see this region's nucleotide sequence