Experiment: R2A with m-arsenite 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2454 and Atu2455 are separated by 70 nucleotides Atu2455 and Atu2456 are separated by 18 nucleotides Atu2456 and Atu2457 are separated by 250 nucleotides Atu2457 and Atu2458 are separated by 72 nucleotides
Atu2454: Atu2454 - iron-chelator utilization protein, at 2,425,266 to 2,426,342
Atu2454
Atu2455: Atu2455 - uracil-DNA glycosylase, at 2,426,413 to 2,427,126
Atu2455
Atu2456: Atu2456 - lactoylglutathione lyase, at 2,427,145 to 2,427,540
Atu2456
Atu2457: Atu2457 - hypothetical protein, at 2,427,791 to 2,428,147
Atu2457
Atu2458: Atu2458 - ABC transporter, nucleotide binding/ATPase protein (hemin), at 2,428,220 to 2,429,002
Atu2458
Position (kb)
2427
2428 Strain fitness (log2 ratio)
-1
0
1
2 at 2426.199 kb on + strand, within Atu2454 at 2426.230 kb on - strand, within Atu2454 at 2426.321 kb on - strand at 2426.500 kb on - strand, within Atu2455 at 2426.561 kb on - strand, within Atu2455 at 2426.608 kb on - strand, within Atu2455 at 2426.613 kb on - strand, within Atu2455 at 2426.629 kb on - strand, within Atu2455 at 2426.695 kb on + strand, within Atu2455 at 2426.695 kb on + strand, within Atu2455 at 2426.872 kb on - strand, within Atu2455 at 2426.873 kb on - strand, within Atu2455 at 2426.899 kb on - strand, within Atu2455 at 2426.970 kb on - strand, within Atu2455 at 2426.985 kb on - strand, within Atu2455 at 2427.039 kb on + strand, within Atu2455 at 2427.242 kb on + strand, within Atu2456 at 2427.261 kb on + strand, within Atu2456 at 2427.269 kb on - strand, within Atu2456 at 2427.335 kb on - strand, within Atu2456 at 2427.412 kb on - strand, within Atu2456 at 2427.412 kb on - strand, within Atu2456 at 2427.529 kb on - strand at 2427.536 kb on + strand at 2427.573 kb on + strand at 2427.578 kb on + strand at 2427.600 kb on + strand at 2427.601 kb on - strand at 2427.601 kb on - strand at 2427.615 kb on - strand at 2427.615 kb on - strand at 2427.652 kb on + strand at 2427.751 kb on + strand at 2427.751 kb on + strand at 2427.751 kb on + strand at 2427.759 kb on - strand at 2427.806 kb on - strand at 2427.818 kb on - strand at 2427.939 kb on + strand, within Atu2457 at 2427.952 kb on - strand, within Atu2457 at 2427.952 kb on - strand, within Atu2457 at 2428.004 kb on + strand, within Atu2457 at 2428.019 kb on + strand, within Atu2457 at 2428.118 kb on - strand at 2428.162 kb on - strand at 2428.184 kb on - strand at 2428.293 kb on + strand at 2428.346 kb on - strand, within Atu2458 at 2428.346 kb on - strand, within Atu2458 at 2428.434 kb on - strand, within Atu2458
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with m-arsenite 0.8 mM remove 2,426,199 + Atu2454 0.87 +0.0 2,426,230 - Atu2454 0.90 -0.1 2,426,321 - +0.8 2,426,500 - Atu2455 0.12 -0.4 2,426,561 - Atu2455 0.21 -0.5 2,426,608 - Atu2455 0.27 -0.2 2,426,613 - Atu2455 0.28 +0.4 2,426,629 - Atu2455 0.30 +0.3 2,426,695 + Atu2455 0.39 -1.2 2,426,695 + Atu2455 0.39 +0.2 2,426,872 - Atu2455 0.64 +1.5 2,426,873 - Atu2455 0.64 +0.2 2,426,899 - Atu2455 0.68 -0.0 2,426,970 - Atu2455 0.78 -1.1 2,426,985 - Atu2455 0.80 +0.0 2,427,039 + Atu2455 0.88 -0.6 2,427,242 + Atu2456 0.24 +0.7 2,427,261 + Atu2456 0.29 +0.0 2,427,269 - Atu2456 0.31 +1.1 2,427,335 - Atu2456 0.48 +0.5 2,427,412 - Atu2456 0.67 +1.7 2,427,412 - Atu2456 0.67 +0.5 2,427,529 - -0.5 2,427,536 + +0.8 2,427,573 + +0.4 2,427,578 + +0.3 2,427,600 + -0.4 2,427,601 - -1.5 2,427,601 - -0.3 2,427,615 - +2.0 2,427,615 - +0.5 2,427,652 + +0.1 2,427,751 + -0.6 2,427,751 + +0.4 2,427,751 + +0.6 2,427,759 - +0.6 2,427,806 - -0.4 2,427,818 - +0.1 2,427,939 + Atu2457 0.41 -0.4 2,427,952 - Atu2457 0.45 +0.9 2,427,952 - Atu2457 0.45 -0.6 2,428,004 + Atu2457 0.60 +1.3 2,428,019 + Atu2457 0.64 +0.2 2,428,118 - +0.5 2,428,162 - -0.0 2,428,184 - +0.5 2,428,293 + +0.3 2,428,346 - Atu2458 0.16 -0.1 2,428,346 - Atu2458 0.16 +0.0 2,428,434 - Atu2458 0.27 +0.3
Or see this region's nucleotide sequence