Experiment: R2A with m-arsenite 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0328 and Atu0329 are separated by 114 nucleotides Atu0329 and Atu0330 are separated by 81 nucleotides Atu0330 and Atu0331 are separated by 82 nucleotides Atu0331 and Atu0332 are separated by 233 nucleotides
Atu0328: Atu0328 - heat-inducible transcription repressor, at 321,899 to 322,990
Atu0328
Atu0329: Atu0329 - GRPE protein, at 323,105 to 323,740
Atu0329
Atu0330: Atu0330 - nitrogen regulatory IIA protein, at 323,822 to 324,283
Atu0330
Atu0331: Atu0331 - sigma-54 modulation protein, at 324,366 to 324,938
Atu0331
Atu0332: Atu0332 - RNA polymerase sigma-54 factor, at 325,172 to 326,716
Atu0332
Position (kb) 323
324
325 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 322.920 kb on - strand at 323.689 kb on - strand at 324.372 kb on - strand at 324.372 kb on - strand at 324.472 kb on - strand, within Atu0331 at 324.624 kb on - strand, within Atu0331 at 324.650 kb on - strand, within Atu0331 at 324.863 kb on + strand, within Atu0331 at 325.101 kb on + strand at 325.109 kb on - strand at 325.124 kb on + strand at 325.226 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with m-arsenite 0.8 mM remove 322,920 - +2.5 323,689 - -3.4 324,372 - -1.5 324,372 - -4.3 324,472 - Atu0331 0.18 -2.4 324,624 - Atu0331 0.45 -0.4 324,650 - Atu0331 0.50 -4.5 324,863 + Atu0331 0.87 -2.3 325,101 + +0.4 325,109 - +0.4 325,124 + -0.1 325,226 + +0.6
Or see this region's nucleotide sequence