Experiment: undecene 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and fliS are separated by 24 nucleotides fliS and fliT overlap by 1 nucleotides fliT and amyA are separated by 77 nucleotides amyA and yedD are separated by 33 nucleotides yedD and yedE are separated by 186 nucleotides
b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302
fliD
b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737
fliS
b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102
fliT
b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667
amyA
b1928: yedD - hypothetical protein (NCBI), at 2,005,701 to 2,006,114
yedD
b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506
yedE
Position (kb)
2004
2005
2006 Strain fitness (log2 ratio)
-2
-1
0
1 at 2003.414 kb on + strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.462 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.699 kb on + strand at 2003.734 kb on + strand at 2003.735 kb on - strand at 2003.738 kb on - strand at 2003.923 kb on - strand, within fliT at 2003.933 kb on - strand, within fliT at 2004.035 kb on + strand, within fliT at 2004.073 kb on + strand at 2004.135 kb on + strand at 2004.148 kb on + strand at 2004.241 kb on + strand at 2004.241 kb on + strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.299 kb on + strand at 2004.446 kb on + strand, within amyA at 2004.461 kb on - strand, within amyA at 2004.533 kb on + strand, within amyA at 2004.543 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.739 kb on - strand, within amyA at 2004.809 kb on + strand, within amyA at 2004.861 kb on - strand, within amyA at 2004.942 kb on - strand, within amyA at 2005.017 kb on - strand, within amyA at 2005.185 kb on + strand, within amyA at 2005.185 kb on + strand, within amyA at 2005.308 kb on + strand, within amyA at 2005.308 kb on + strand, within amyA at 2005.310 kb on + strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.443 kb on - strand, within amyA at 2005.591 kb on - strand at 2005.633 kb on + strand at 2005.633 kb on + strand at 2005.659 kb on - strand at 2005.659 kb on - strand at 2005.729 kb on + strand at 2005.787 kb on - strand, within yedD at 2005.788 kb on + strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.809 kb on - strand, within yedD at 2005.827 kb on - strand, within yedD at 2005.953 kb on + strand, within yedD at 2005.969 kb on - strand, within yedD at 2006.256 kb on - strand at 2006.284 kb on + strand at 2006.393 kb on - strand at 2006.393 kb on - strand at 2006.417 kb on + strand at 2006.417 kb on + strand at 2006.457 kb on - strand, within yedE at 2006.508 kb on + strand, within yedE at 2006.605 kb on - strand, within yedE
Per-strain Table
Position Strand Gene LocusTag Fraction undecene 2.5 vol% remove 2,003,414 + fliS b1925 0.21 -0.1 2,003,451 - fliS b1925 0.30 +0.8 2,003,451 - fliS b1925 0.30 +0.4 2,003,451 - fliS b1925 0.30 +0.7 2,003,462 + fliS b1925 0.33 +0.2 2,003,544 + fliS b1925 0.53 +0.2 2,003,544 + fliS b1925 0.53 +0.3 2,003,699 + +0.4 2,003,734 + -0.4 2,003,735 - +0.5 2,003,738 - +0.4 2,003,923 - fliT b1926 0.51 +0.4 2,003,933 - fliT b1926 0.54 +0.1 2,004,035 + fliT b1926 0.81 +0.6 2,004,073 + +0.1 2,004,135 + +0.1 2,004,148 + +0.5 2,004,241 + +0.0 2,004,241 + +0.9 2,004,249 - +0.5 2,004,249 - -0.0 2,004,249 - +0.1 2,004,299 + +0.2 2,004,446 + amyA b1927 0.18 -1.5 2,004,461 - amyA b1927 0.19 +0.4 2,004,533 + amyA b1927 0.24 -0.1 2,004,543 - amyA b1927 0.24 +0.8 2,004,614 - amyA b1927 0.29 +0.3 2,004,614 - amyA b1927 0.29 +0.9 2,004,739 - amyA b1927 0.38 +0.1 2,004,809 + amyA b1927 0.42 +0.1 2,004,861 - amyA b1927 0.46 +1.0 2,004,942 - amyA b1927 0.51 -0.2 2,005,017 - amyA b1927 0.56 -0.1 2,005,185 + amyA b1927 0.68 +1.0 2,005,185 + amyA b1927 0.68 +0.8 2,005,308 + amyA b1927 0.76 -2.0 2,005,308 + amyA b1927 0.76 +0.1 2,005,310 + amyA b1927 0.76 -0.9 2,005,410 - amyA b1927 0.83 -0.5 2,005,410 - amyA b1927 0.83 -0.2 2,005,443 - amyA b1927 0.85 -0.2 2,005,591 - -0.5 2,005,633 + -0.8 2,005,633 + +0.6 2,005,659 - -0.2 2,005,659 - -0.1 2,005,729 + -0.7 2,005,787 - yedD b1928 0.21 +0.1 2,005,788 + yedD b1928 0.21 -0.9 2,005,801 - yedD b1928 0.24 -0.8 2,005,801 - yedD b1928 0.24 -0.2 2,005,809 - yedD b1928 0.26 -0.7 2,005,827 - yedD b1928 0.30 -1.2 2,005,953 + yedD b1928 0.61 -0.7 2,005,969 - yedD b1928 0.65 +0.2 2,006,256 - +0.8 2,006,284 + +1.3 2,006,393 - +0.1 2,006,393 - -0.7 2,006,417 + +0.8 2,006,417 + +0.3 2,006,457 - yedE b1929 0.13 +0.1 2,006,508 + yedE b1929 0.17 +0.1 2,006,605 - yedE b1929 0.25 -0.7
Or see this region's nucleotide sequence