Experiment: R2A with vanadium chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4647 and Atu4648 are separated by 14 nucleotides Atu4648 and Atu4649 are separated by 15 nucleotides Atu4649 and Atu4650 overlap by 4 nucleotides
Atu4647: Atu4647 - hypothetical protein, at 1,800,329 to 1,801,282
Atu4647
Atu4648: Atu4648 - choline sulfatase, at 1,801,297 to 1,802,808
Atu4648
Atu4649: Atu4649 - carbohydrate kinase, at 1,802,824 to 1,803,717
Atu4649
Atu4650: Atu4650 - sugar ABC transporter ATPase, at 1,803,714 to 1,804,754
Atu4650
Position (kb)
1801
1802
1803 Strain fitness (log2 ratio)
-1
0
1 at 1800.311 kb on + strand at 1800.374 kb on - strand at 1800.374 kb on - strand at 1800.378 kb on - strand at 1800.516 kb on - strand, within Atu4647 at 1800.523 kb on + strand, within Atu4647 at 1800.558 kb on - strand, within Atu4647 at 1800.667 kb on + strand, within Atu4647 at 1800.700 kb on + strand, within Atu4647 at 1800.708 kb on - strand, within Atu4647 at 1800.812 kb on + strand, within Atu4647 at 1800.888 kb on - strand, within Atu4647 at 1800.895 kb on + strand, within Atu4647 at 1800.903 kb on - strand, within Atu4647 at 1800.913 kb on + strand, within Atu4647 at 1801.023 kb on + strand, within Atu4647 at 1801.273 kb on - strand at 1801.307 kb on + strand at 1801.417 kb on - strand at 1801.431 kb on - strand at 1801.431 kb on - strand at 1801.476 kb on - strand, within Atu4648 at 1801.478 kb on - strand, within Atu4648 at 1801.628 kb on + strand, within Atu4648 at 1801.628 kb on + strand, within Atu4648 at 1801.636 kb on - strand, within Atu4648 at 1801.636 kb on - strand, within Atu4648 at 1801.636 kb on - strand, within Atu4648 at 1801.777 kb on + strand, within Atu4648 at 1801.777 kb on + strand, within Atu4648 at 1801.824 kb on - strand, within Atu4648 at 1802.028 kb on + strand, within Atu4648 at 1802.213 kb on + strand, within Atu4648 at 1802.306 kb on + strand, within Atu4648 at 1802.316 kb on + strand, within Atu4648 at 1802.422 kb on + strand, within Atu4648 at 1802.441 kb on + strand, within Atu4648 at 1802.519 kb on - strand, within Atu4648 at 1802.666 kb on + strand at 1802.673 kb on + strand at 1802.681 kb on - strand at 1802.704 kb on - strand at 1802.722 kb on + strand at 1802.722 kb on + strand at 1802.730 kb on - strand at 1802.762 kb on + strand at 1802.771 kb on - strand at 1802.771 kb on - strand at 1802.812 kb on + strand at 1802.876 kb on - strand at 1802.894 kb on + strand at 1802.964 kb on - strand, within Atu4649 at 1803.010 kb on - strand, within Atu4649 at 1803.026 kb on + strand, within Atu4649 at 1803.033 kb on + strand, within Atu4649 at 1803.033 kb on + strand, within Atu4649 at 1803.041 kb on - strand, within Atu4649 at 1803.061 kb on - strand, within Atu4649 at 1803.108 kb on + strand, within Atu4649 at 1803.184 kb on - strand, within Atu4649 at 1803.184 kb on - strand, within Atu4649 at 1803.184 kb on - strand, within Atu4649 at 1803.194 kb on - strand, within Atu4649 at 1803.199 kb on + strand, within Atu4649 at 1803.207 kb on - strand, within Atu4649 at 1803.347 kb on + strand, within Atu4649 at 1803.361 kb on - strand, within Atu4649 at 1803.391 kb on + strand, within Atu4649 at 1803.454 kb on + strand, within Atu4649 at 1803.460 kb on + strand, within Atu4649 at 1803.512 kb on + strand, within Atu4649 at 1803.512 kb on + strand, within Atu4649 at 1803.520 kb on - strand, within Atu4649 at 1803.626 kb on - strand, within Atu4649 at 1803.681 kb on - strand at 1803.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with vanadium chloride 0.08 mM remove 1,800,311 + +0.2 1,800,374 - +0.0 1,800,374 - -0.0 1,800,378 - -0.3 1,800,516 - Atu4647 0.20 -0.2 1,800,523 + Atu4647 0.20 -0.4 1,800,558 - Atu4647 0.24 -0.1 1,800,667 + Atu4647 0.35 +0.1 1,800,700 + Atu4647 0.39 +1.0 1,800,708 - Atu4647 0.40 -0.4 1,800,812 + Atu4647 0.51 -0.1 1,800,888 - Atu4647 0.59 +0.3 1,800,895 + Atu4647 0.59 -0.2 1,800,903 - Atu4647 0.60 -0.6 1,800,913 + Atu4647 0.61 +0.3 1,801,023 + Atu4647 0.73 +0.9 1,801,273 - +0.8 1,801,307 + +0.2 1,801,417 - -0.1 1,801,431 - -0.1 1,801,431 - -0.3 1,801,476 - Atu4648 0.12 +0.3 1,801,478 - Atu4648 0.12 +0.2 1,801,628 + Atu4648 0.22 +0.5 1,801,628 + Atu4648 0.22 -0.2 1,801,636 - Atu4648 0.22 +0.5 1,801,636 - Atu4648 0.22 +0.1 1,801,636 - Atu4648 0.22 +1.3 1,801,777 + Atu4648 0.32 -0.2 1,801,777 + Atu4648 0.32 -0.1 1,801,824 - Atu4648 0.35 +0.3 1,802,028 + Atu4648 0.48 +1.7 1,802,213 + Atu4648 0.61 +0.4 1,802,306 + Atu4648 0.67 +0.3 1,802,316 + Atu4648 0.67 +0.8 1,802,422 + Atu4648 0.74 +0.0 1,802,441 + Atu4648 0.76 -0.8 1,802,519 - Atu4648 0.81 +0.2 1,802,666 + +0.4 1,802,673 + -0.4 1,802,681 - -1.0 1,802,704 - +0.3 1,802,722 + -0.6 1,802,722 + +0.2 1,802,730 - -0.2 1,802,762 + -0.0 1,802,771 - +0.6 1,802,771 - -0.5 1,802,812 + -0.1 1,802,876 - +0.9 1,802,894 + -0.7 1,802,964 - Atu4649 0.16 -0.1 1,803,010 - Atu4649 0.21 -0.4 1,803,026 + Atu4649 0.23 +0.7 1,803,033 + Atu4649 0.23 +0.3 1,803,033 + Atu4649 0.23 +1.0 1,803,041 - Atu4649 0.24 +0.2 1,803,061 - Atu4649 0.27 +0.3 1,803,108 + Atu4649 0.32 +0.8 1,803,184 - Atu4649 0.40 -0.0 1,803,184 - Atu4649 0.40 -0.1 1,803,184 - Atu4649 0.40 +0.1 1,803,194 - Atu4649 0.41 +0.3 1,803,199 + Atu4649 0.42 -0.1 1,803,207 - Atu4649 0.43 -0.7 1,803,347 + Atu4649 0.59 -1.0 1,803,361 - Atu4649 0.60 +0.2 1,803,391 + Atu4649 0.63 +0.9 1,803,454 + Atu4649 0.70 -0.8 1,803,460 + Atu4649 0.71 +0.7 1,803,512 + Atu4649 0.77 +0.6 1,803,512 + Atu4649 0.77 -0.2 1,803,520 - Atu4649 0.78 -0.1 1,803,626 - Atu4649 0.90 +0.2 1,803,681 - -0.5 1,803,731 - +0.2
Or see this region's nucleotide sequence