Experiment: undecene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdsD and kdsC are separated by 20 nucleotides kdsC and yrbK overlap by 4 nucleotides yrbK and yhbN overlap by 32 nucleotides yhbN and yhbG are separated by 6 nucleotides
b3197: kdsD - D-arabinose 5-phosphate isomerase (NCBI), at 3,339,288 to 3,340,274
kdsD
b3198: kdsC - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI), at 3,340,295 to 3,340,861
kdsC
b3199: yrbK - hypothetical protein (NCBI), at 3,340,858 to 3,341,433
yrbK
b3200: yhbN - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 3,341,402 to 3,341,959
yhbN
b3201: yhbG - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 3,341,966 to 3,342,691
yhbG
Position (kb)
3340
3341
3342 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3339.864 kb on + strand, within kdsD at 3339.868 kb on - strand, within kdsD at 3339.876 kb on + strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.924 kb on + strand, within kdsD at 3339.974 kb on + strand, within kdsD at 3340.032 kb on + strand, within kdsD at 3340.105 kb on - strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.203 kb on + strand at 3340.203 kb on + strand at 3340.231 kb on + strand at 3340.269 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.374 kb on + strand, within kdsC at 3340.374 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.736 kb on + strand, within kdsC at 3341.218 kb on + strand, within yrbK at 3341.218 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK at 3341.388 kb on + strand at 3341.388 kb on + strand at 3341.944 kb on + strand at 3341.944 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction undecene 5 vol% remove 3,339,864 + kdsD b3197 0.58 -0.8 3,339,868 - kdsD b3197 0.59 -2.6 3,339,876 + kdsD b3197 0.60 -1.5 3,339,879 - kdsD b3197 0.60 -3.0 3,339,879 - kdsD b3197 0.60 -3.5 3,339,924 + kdsD b3197 0.64 -1.6 3,339,974 + kdsD b3197 0.70 -2.1 3,340,032 + kdsD b3197 0.75 -1.9 3,340,105 - kdsD b3197 0.83 -1.6 3,340,129 + kdsD b3197 0.85 -2.3 3,340,129 + kdsD b3197 0.85 -0.8 3,340,203 + -1.1 3,340,203 + -2.5 3,340,231 + -2.6 3,340,269 + -2.7 3,340,326 + -2.2 3,340,326 + -2.1 3,340,326 + -2.9 3,340,374 + kdsC b3198 0.14 -3.0 3,340,374 + kdsC b3198 0.14 -2.5 3,340,391 + kdsC b3198 0.17 -2.2 3,340,391 + kdsC b3198 0.17 -2.7 3,340,736 + kdsC b3198 0.78 -1.7 3,341,218 + yrbK b3199 0.62 -1.6 3,341,218 + yrbK b3199 0.62 -1.9 3,341,256 + yrbK b3199 0.69 -3.2 3,341,256 + yrbK b3199 0.69 -4.0 3,341,388 + -1.1 3,341,388 + -3.8 3,341,944 + -3.1 3,341,944 + -3.3
Or see this region's nucleotide sequence