Strain Fitness in Escherichia coli BW25113 around b1130

Experiment: undecene 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphoQ and phoP overlap by 1 nucleotidesphoP and purB are separated by 168 nucleotides b1129: phoQ - sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI), at 1,187,539 to 1,188,999 phoQ b1130: phoP - DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI), at 1,188,999 to 1,189,670 phoP b1131: purB - adenylosuccinate lyase (NCBI), at 1,189,839 to 1,191,209 purB Position (kb) 1188 1189 1190Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1188.100 kb on - strand, within phoQat 1188.191 kb on + strand, within phoQat 1188.246 kb on + strand, within phoQat 1188.320 kb on + strand, within phoQat 1188.342 kb on + strand, within phoQat 1188.342 kb on + strand, within phoQat 1188.435 kb on - strand, within phoQat 1188.435 kb on - strand, within phoQat 1188.461 kb on + strand, within phoQat 1188.564 kb on + strand, within phoQat 1188.760 kb on - strand, within phoQat 1188.760 kb on - strand, within phoQat 1188.911 kb on + strandat 1188.924 kb on + strandat 1189.023 kb on - strandat 1189.104 kb on + strand, within phoPat 1189.121 kb on + strand, within phoPat 1189.135 kb on - strand, within phoPat 1189.135 kb on - strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.320 kb on - strand, within phoPat 1189.320 kb on - strand, within phoPat 1189.330 kb on - strand, within phoPat 1189.375 kb on - strand, within phoPat 1189.375 kb on - strand, within phoPat 1189.395 kb on + strand, within phoPat 1189.403 kb on - strand, within phoPat 1189.483 kb on + strand, within phoPat 1189.587 kb on + strand, within phoPat 1189.587 kb on + strand, within phoPat 1189.590 kb on + strand, within phoPat 1189.614 kb on - strandat 1189.713 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction undecene 5 vol%
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1,188,100 - phoQ b1129 0.38 -2.3
1,188,191 + phoQ b1129 0.45 -3.6
1,188,246 + phoQ b1129 0.48 -3.8
1,188,320 + phoQ b1129 0.53 -4.1
1,188,342 + phoQ b1129 0.55 -3.9
1,188,342 + phoQ b1129 0.55 -3.0
1,188,435 - phoQ b1129 0.61 -2.8
1,188,435 - phoQ b1129 0.61 -3.2
1,188,461 + phoQ b1129 0.63 -4.0
1,188,564 + phoQ b1129 0.70 -2.9
1,188,760 - phoQ b1129 0.84 -3.4
1,188,760 - phoQ b1129 0.84 -3.4
1,188,911 + -2.6
1,188,924 + -2.9
1,189,023 - +0.1
1,189,104 + phoP b1130 0.16 -3.7
1,189,121 + phoP b1130 0.18 -3.8
1,189,135 - phoP b1130 0.20 -4.2
1,189,135 - phoP b1130 0.20 -3.9
1,189,137 + phoP b1130 0.21 -4.0
1,189,137 + phoP b1130 0.21 -4.2
1,189,137 + phoP b1130 0.21 -3.0
1,189,320 - phoP b1130 0.48 -3.1
1,189,320 - phoP b1130 0.48 -1.5
1,189,330 - phoP b1130 0.49 -3.9
1,189,375 - phoP b1130 0.56 -2.7
1,189,375 - phoP b1130 0.56 -1.9
1,189,395 + phoP b1130 0.59 -4.3
1,189,403 - phoP b1130 0.60 -1.7
1,189,483 + phoP b1130 0.72 -3.8
1,189,587 + phoP b1130 0.88 -3.1
1,189,587 + phoP b1130 0.88 -2.8
1,189,590 + phoP b1130 0.88 -4.5
1,189,614 - -2.8
1,189,713 + -2.1

Or see this region's nucleotide sequence