Experiment: undecene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mtgA and yhbL overlap by 4 nucleotides yhbL and arcZ are separated by 117 nucleotides arcZ and arcB overlap by 9 nucleotides arcB and yhcC are separated by 95 nucleotides
b3208: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI), at 3,347,103 to 3,347,831
mtgA
b3209: yhbL - sigma cross-reacting protein 27A (SCRP-27A) (VIMSS), at 3,347,828 to 3,348,481
yhbL
b4450: arcZ - sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq), at 3,348,599 to 3,348,719
arcZ
b3210: arcB - hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI), at 3,348,711 to 3,351,047
arcB
b3211: yhcC - predicted Fe-S oxidoreductase (NCBI), at 3,351,143 to 3,352,072
yhcC
Position (kb)
3348
3349
3350
3351
3352 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3347.713 kb on + strand, within mtgA at 3347.725 kb on + strand, within mtgA at 3347.725 kb on + strand, within mtgA at 3347.738 kb on - strand, within mtgA at 3347.778 kb on - strand at 3347.820 kb on - strand at 3347.904 kb on + strand, within yhbL at 3347.904 kb on + strand, within yhbL at 3347.911 kb on - strand, within yhbL at 3347.916 kb on - strand, within yhbL at 3347.975 kb on - strand, within yhbL at 3348.075 kb on + strand, within yhbL at 3348.193 kb on + strand, within yhbL at 3348.193 kb on + strand, within yhbL at 3348.211 kb on + strand, within yhbL at 3348.211 kb on - strand, within yhbL at 3348.240 kb on + strand, within yhbL at 3348.240 kb on + strand, within yhbL at 3348.240 kb on + strand, within yhbL at 3348.245 kb on + strand, within yhbL at 3348.245 kb on + strand, within yhbL at 3348.248 kb on - strand, within yhbL at 3348.248 kb on - strand, within yhbL at 3348.287 kb on + strand, within yhbL at 3348.287 kb on + strand, within yhbL at 3348.313 kb on + strand, within yhbL at 3348.313 kb on + strand, within yhbL at 3348.322 kb on - strand, within yhbL at 3348.332 kb on - strand, within yhbL at 3348.347 kb on - strand, within yhbL at 3348.369 kb on - strand, within yhbL at 3348.384 kb on - strand, within yhbL at 3348.384 kb on - strand, within yhbL at 3348.445 kb on - strand at 3348.450 kb on + strand at 3348.483 kb on + strand at 3348.490 kb on + strand at 3348.490 kb on + strand at 3348.493 kb on + strand at 3348.598 kb on - strand at 3348.598 kb on - strand at 3348.681 kb on - strand, within arcZ at 3348.681 kb on - strand, within arcZ at 3348.683 kb on + strand, within arcZ at 3348.692 kb on - strand, within arcZ at 3348.707 kb on - strand, within arcZ at 3348.780 kb on - strand at 3348.821 kb on + strand at 3348.822 kb on - strand at 3348.836 kb on - strand at 3348.871 kb on + strand at 3349.074 kb on + strand, within arcB at 3349.074 kb on + strand, within arcB at 3349.074 kb on + strand, within arcB at 3349.079 kb on + strand, within arcB at 3349.082 kb on - strand, within arcB at 3349.082 kb on - strand, within arcB at 3349.089 kb on - strand, within arcB at 3349.098 kb on + strand, within arcB at 3349.098 kb on - strand, within arcB at 3349.106 kb on - strand, within arcB at 3349.106 kb on - strand, within arcB at 3349.106 kb on - strand, within arcB at 3349.115 kb on - strand, within arcB at 3349.154 kb on - strand, within arcB at 3349.180 kb on - strand, within arcB at 3349.180 kb on - strand, within arcB at 3349.189 kb on + strand, within arcB at 3349.189 kb on + strand, within arcB at 3349.298 kb on - strand, within arcB at 3349.359 kb on + strand, within arcB at 3349.440 kb on + strand, within arcB at 3349.450 kb on - strand, within arcB at 3349.540 kb on - strand, within arcB at 3349.562 kb on - strand, within arcB at 3349.569 kb on + strand, within arcB at 3349.591 kb on - strand, within arcB at 3349.639 kb on - strand, within arcB at 3349.737 kb on - strand, within arcB at 3349.764 kb on + strand, within arcB at 3349.779 kb on - strand, within arcB at 3349.898 kb on + strand, within arcB at 3350.002 kb on + strand, within arcB at 3350.002 kb on + strand, within arcB at 3350.010 kb on - strand, within arcB at 3350.128 kb on - strand, within arcB at 3350.128 kb on - strand, within arcB at 3350.141 kb on - strand, within arcB at 3350.149 kb on + strand, within arcB at 3350.151 kb on + strand, within arcB at 3350.151 kb on - strand, within arcB at 3350.178 kb on + strand, within arcB at 3350.206 kb on + strand, within arcB at 3350.206 kb on + strand, within arcB at 3350.216 kb on + strand, within arcB at 3350.216 kb on + strand, within arcB at 3350.303 kb on - strand, within arcB at 3350.303 kb on - strand, within arcB at 3350.357 kb on + strand, within arcB at 3350.357 kb on + strand, within arcB at 3350.360 kb on + strand, within arcB at 3350.487 kb on + strand, within arcB at 3350.574 kb on + strand, within arcB at 3350.810 kb on + strand, within arcB at 3350.848 kb on + strand at 3351.008 kb on + strand at 3351.013 kb on + strand at 3351.013 kb on + strand at 3351.018 kb on + strand at 3351.021 kb on - strand at 3351.021 kb on - strand at 3351.021 kb on - strand at 3351.021 kb on - strand at 3351.026 kb on - strand at 3351.057 kb on - strand at 3351.057 kb on - strand at 3351.140 kb on + strand at 3351.140 kb on + strand at 3351.347 kb on - strand, within yhcC at 3351.361 kb on + strand, within yhcC at 3351.369 kb on - strand, within yhcC at 3351.369 kb on - strand, within yhcC at 3351.422 kb on + strand, within yhcC at 3351.436 kb on + strand, within yhcC at 3351.564 kb on + strand, within yhcC at 3351.564 kb on + strand, within yhcC at 3351.570 kb on - strand, within yhcC at 3351.572 kb on - strand, within yhcC at 3351.572 kb on - strand, within yhcC at 3351.580 kb on + strand, within yhcC at 3351.597 kb on + strand, within yhcC at 3351.649 kb on + strand, within yhcC at 3351.649 kb on + strand, within yhcC at 3351.655 kb on - strand, within yhcC at 3351.657 kb on - strand, within yhcC at 3351.699 kb on + strand, within yhcC at 3351.750 kb on + strand, within yhcC at 3351.839 kb on - strand, within yhcC at 3351.952 kb on + strand, within yhcC at 3351.960 kb on - strand, within yhcC
Per-strain Table
Position Strand Gene LocusTag Fraction undecene 5 vol% remove 3,347,713 + mtgA b3208 0.84 -0.6 3,347,725 + mtgA b3208 0.85 -0.7 3,347,725 + mtgA b3208 0.85 -0.9 3,347,738 - mtgA b3208 0.87 +0.4 3,347,778 - -2.1 3,347,820 - -0.3 3,347,904 + yhbL b3209 0.12 -0.3 3,347,904 + yhbL b3209 0.12 +0.1 3,347,911 - yhbL b3209 0.13 +0.5 3,347,916 - yhbL b3209 0.13 -0.7 3,347,975 - yhbL b3209 0.22 +1.4 3,348,075 + yhbL b3209 0.38 +0.1 3,348,193 + yhbL b3209 0.56 -0.7 3,348,193 + yhbL b3209 0.56 +1.0 3,348,211 + yhbL b3209 0.59 +0.5 3,348,211 - yhbL b3209 0.59 -0.5 3,348,240 + yhbL b3209 0.63 -0.5 3,348,240 + yhbL b3209 0.63 -0.5 3,348,240 + yhbL b3209 0.63 +0.7 3,348,245 + yhbL b3209 0.64 -0.1 3,348,245 + yhbL b3209 0.64 +0.1 3,348,248 - yhbL b3209 0.64 +0.5 3,348,248 - yhbL b3209 0.64 -1.0 3,348,287 + yhbL b3209 0.70 +0.4 3,348,287 + yhbL b3209 0.70 +0.8 3,348,313 + yhbL b3209 0.74 -0.8 3,348,313 + yhbL b3209 0.74 +0.1 3,348,322 - yhbL b3209 0.76 +0.7 3,348,332 - yhbL b3209 0.77 +0.5 3,348,347 - yhbL b3209 0.79 +0.8 3,348,369 - yhbL b3209 0.83 -0.5 3,348,384 - yhbL b3209 0.85 +0.8 3,348,384 - yhbL b3209 0.85 +0.2 3,348,445 - -1.1 3,348,450 + +0.7 3,348,483 + -0.8 3,348,490 + +0.7 3,348,490 + +0.6 3,348,493 + +0.2 3,348,598 - +1.1 3,348,598 - +1.8 3,348,681 - arcZ b4450 0.68 +0.2 3,348,681 - arcZ b4450 0.68 -0.3 3,348,683 + arcZ b4450 0.69 -0.0 3,348,692 - arcZ b4450 0.77 +0.2 3,348,707 - arcZ b4450 0.89 -0.3 3,348,780 - -2.1 3,348,821 + -1.6 3,348,822 - -2.3 3,348,836 - -1.6 3,348,871 + -1.3 3,349,074 + arcB b3210 0.16 -0.7 3,349,074 + arcB b3210 0.16 -0.8 3,349,074 + arcB b3210 0.16 -0.8 3,349,079 + arcB b3210 0.16 -2.3 3,349,082 - arcB b3210 0.16 -2.2 3,349,082 - arcB b3210 0.16 -0.7 3,349,089 - arcB b3210 0.16 -0.7 3,349,098 + arcB b3210 0.17 -0.3 3,349,098 - arcB b3210 0.17 -1.1 3,349,106 - arcB b3210 0.17 -2.1 3,349,106 - arcB b3210 0.17 -1.0 3,349,106 - arcB b3210 0.17 -2.1 3,349,115 - arcB b3210 0.17 -1.7 3,349,154 - arcB b3210 0.19 -1.0 3,349,180 - arcB b3210 0.20 -1.4 3,349,180 - arcB b3210 0.20 -0.2 3,349,189 + arcB b3210 0.20 -2.7 3,349,189 + arcB b3210 0.20 -0.4 3,349,298 - arcB b3210 0.25 -1.9 3,349,359 + arcB b3210 0.28 -0.9 3,349,440 + arcB b3210 0.31 -0.9 3,349,450 - arcB b3210 0.32 -1.2 3,349,540 - arcB b3210 0.35 -1.2 3,349,562 - arcB b3210 0.36 -0.8 3,349,569 + arcB b3210 0.37 -1.7 3,349,591 - arcB b3210 0.38 -2.1 3,349,639 - arcB b3210 0.40 +0.2 3,349,737 - arcB b3210 0.44 -0.6 3,349,764 + arcB b3210 0.45 -1.1 3,349,779 - arcB b3210 0.46 -1.4 3,349,898 + arcB b3210 0.51 +0.4 3,350,002 + arcB b3210 0.55 -0.2 3,350,002 + arcB b3210 0.55 -0.5 3,350,010 - arcB b3210 0.56 -1.8 3,350,128 - arcB b3210 0.61 -1.1 3,350,128 - arcB b3210 0.61 -1.8 3,350,141 - arcB b3210 0.61 -1.6 3,350,149 + arcB b3210 0.62 -2.1 3,350,151 + arcB b3210 0.62 -1.8 3,350,151 - arcB b3210 0.62 -1.2 3,350,178 + arcB b3210 0.63 -2.2 3,350,206 + arcB b3210 0.64 -0.9 3,350,206 + arcB b3210 0.64 -1.4 3,350,216 + arcB b3210 0.64 -3.2 3,350,216 + arcB b3210 0.64 -1.2 3,350,303 - arcB b3210 0.68 -1.6 3,350,303 - arcB b3210 0.68 -1.6 3,350,357 + arcB b3210 0.70 -1.5 3,350,357 + arcB b3210 0.70 -0.2 3,350,360 + arcB b3210 0.71 -0.4 3,350,487 + arcB b3210 0.76 -2.3 3,350,574 + arcB b3210 0.80 -0.4 3,350,810 + arcB b3210 0.90 -2.9 3,350,848 + -0.2 3,351,008 + +0.1 3,351,013 + -1.2 3,351,013 + +0.5 3,351,018 + -2.2 3,351,021 - -1.3 3,351,021 - -0.2 3,351,021 - -0.4 3,351,021 - -0.3 3,351,026 - -1.8 3,351,057 - -1.1 3,351,057 - -0.7 3,351,140 + -1.2 3,351,140 + +0.4 3,351,347 - yhcC b3211 0.22 +0.9 3,351,361 + yhcC b3211 0.23 +0.2 3,351,369 - yhcC b3211 0.24 -0.6 3,351,369 - yhcC b3211 0.24 +0.7 3,351,422 + yhcC b3211 0.30 -0.3 3,351,436 + yhcC b3211 0.32 +0.4 3,351,564 + yhcC b3211 0.45 -0.4 3,351,564 + yhcC b3211 0.45 +0.2 3,351,570 - yhcC b3211 0.46 -0.2 3,351,572 - yhcC b3211 0.46 -2.4 3,351,572 - yhcC b3211 0.46 +0.2 3,351,580 + yhcC b3211 0.47 +1.0 3,351,597 + yhcC b3211 0.49 +0.1 3,351,649 + yhcC b3211 0.54 -0.2 3,351,649 + yhcC b3211 0.54 -0.8 3,351,655 - yhcC b3211 0.55 +0.3 3,351,657 - yhcC b3211 0.55 -0.7 3,351,699 + yhcC b3211 0.60 +0.4 3,351,750 + yhcC b3211 0.65 +0.1 3,351,839 - yhcC b3211 0.75 +0.1 3,351,952 + yhcC b3211 0.87 +0.6 3,351,960 - yhcC b3211 0.88 -0.8
Or see this region's nucleotide sequence