Experiment: undecene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metA and aceB are separated by 268 nucleotides aceB and aceA are separated by 29 nucleotides
b4013: metA - homoserine O-succinyltransferase (NCBI), at 4,212,303 to 4,213,232
metA
b4014: aceB - malate synthase (NCBI), at 4,213,501 to 4,215,102
aceB
b4015: aceA - isocitrate lyase (NCBI), at 4,215,132 to 4,216,436
aceA
Position (kb)
4213
4214
4215
4216 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4212.689 kb on - strand, within metA at 4212.908 kb on - strand, within metA at 4212.908 kb on - strand, within metA at 4212.957 kb on + strand, within metA at 4213.008 kb on + strand, within metA at 4213.008 kb on + strand, within metA at 4213.008 kb on + strand, within metA at 4213.061 kb on - strand, within metA at 4213.068 kb on + strand, within metA at 4213.201 kb on + strand at 4213.491 kb on + strand at 4213.648 kb on + strand at 4213.765 kb on - strand, within aceB at 4213.777 kb on + strand, within aceB at 4213.777 kb on + strand, within aceB at 4213.976 kb on + strand, within aceB at 4214.093 kb on + strand, within aceB at 4214.104 kb on + strand, within aceB at 4214.109 kb on + strand, within aceB at 4214.182 kb on + strand, within aceB at 4214.190 kb on - strand, within aceB at 4214.244 kb on + strand, within aceB at 4214.244 kb on + strand, within aceB at 4214.314 kb on - strand, within aceB at 4214.527 kb on + strand, within aceB at 4214.568 kb on + strand, within aceB at 4214.602 kb on + strand, within aceB at 4214.621 kb on + strand, within aceB at 4214.731 kb on + strand, within aceB at 4214.731 kb on + strand, within aceB at 4214.751 kb on - strand, within aceB at 4214.778 kb on + strand, within aceB at 4215.008 kb on + strand at 4215.037 kb on + strand at 4215.077 kb on + strand at 4215.206 kb on + strand at 4215.214 kb on - strand at 4215.214 kb on - strand at 4215.214 kb on - strand at 4215.214 kb on - strand at 4215.214 kb on - strand at 4215.267 kb on + strand, within aceA at 4215.454 kb on - strand, within aceA at 4215.566 kb on + strand, within aceA at 4215.702 kb on + strand, within aceA at 4215.736 kb on - strand, within aceA at 4215.759 kb on + strand, within aceA at 4215.813 kb on + strand, within aceA at 4215.878 kb on - strand, within aceA at 4215.878 kb on - strand, within aceA at 4215.941 kb on - strand, within aceA at 4215.965 kb on + strand, within aceA at 4215.989 kb on - strand, within aceA at 4215.989 kb on - strand, within aceA at 4216.035 kb on + strand, within aceA at 4216.074 kb on + strand, within aceA at 4216.074 kb on + strand, within aceA at 4216.082 kb on - strand, within aceA
Per-strain Table
Position Strand Gene LocusTag Fraction undecene 5 vol% remove 4,212,689 - metA b4013 0.42 -0.0 4,212,908 - metA b4013 0.65 -0.2 4,212,908 - metA b4013 0.65 +0.6 4,212,957 + metA b4013 0.70 +0.8 4,213,008 + metA b4013 0.76 -0.1 4,213,008 + metA b4013 0.76 +0.3 4,213,008 + metA b4013 0.76 +0.4 4,213,061 - metA b4013 0.82 -0.9 4,213,068 + metA b4013 0.82 +0.4 4,213,201 + -0.6 4,213,491 + -0.5 4,213,648 + +0.5 4,213,765 - aceB b4014 0.16 -0.1 4,213,777 + aceB b4014 0.17 +0.2 4,213,777 + aceB b4014 0.17 -0.0 4,213,976 + aceB b4014 0.30 -0.4 4,214,093 + aceB b4014 0.37 +0.4 4,214,104 + aceB b4014 0.38 -0.0 4,214,109 + aceB b4014 0.38 +0.8 4,214,182 + aceB b4014 0.43 +0.4 4,214,190 - aceB b4014 0.43 -0.1 4,214,244 + aceB b4014 0.46 -0.7 4,214,244 + aceB b4014 0.46 -0.0 4,214,314 - aceB b4014 0.51 -0.8 4,214,527 + aceB b4014 0.64 +1.1 4,214,568 + aceB b4014 0.67 +0.4 4,214,602 + aceB b4014 0.69 +0.1 4,214,621 + aceB b4014 0.70 +0.1 4,214,731 + aceB b4014 0.77 +0.3 4,214,731 + aceB b4014 0.77 +0.2 4,214,751 - aceB b4014 0.78 +0.3 4,214,778 + aceB b4014 0.80 -0.1 4,215,008 + -0.7 4,215,037 + -1.9 4,215,077 + +1.3 4,215,206 + -0.3 4,215,214 - +0.3 4,215,214 - +0.9 4,215,214 - +1.2 4,215,214 - -0.1 4,215,214 - +0.6 4,215,267 + aceA b4015 0.10 -0.1 4,215,454 - aceA b4015 0.25 +1.3 4,215,566 + aceA b4015 0.33 +3.7 4,215,702 + aceA b4015 0.44 +0.1 4,215,736 - aceA b4015 0.46 +0.4 4,215,759 + aceA b4015 0.48 +0.6 4,215,813 + aceA b4015 0.52 +0.3 4,215,878 - aceA b4015 0.57 -0.2 4,215,878 - aceA b4015 0.57 -0.3 4,215,941 - aceA b4015 0.62 +0.3 4,215,965 + aceA b4015 0.64 +0.2 4,215,989 - aceA b4015 0.66 -2.4 4,215,989 - aceA b4015 0.66 +1.2 4,216,035 + aceA b4015 0.69 -0.2 4,216,074 + aceA b4015 0.72 -0.1 4,216,074 + aceA b4015 0.72 +0.8 4,216,082 - aceA b4015 0.73 -0.6
Or see this region's nucleotide sequence